Union Biometrica, Inc. to Present at 3D Cell Culture 2021: Models, Applications & Translation Conference in Freiburg, Germany from May 5-7, 2021.
We invite you to learn more about our all of our technologies by joining us at the following poster:
Title: Analysis, imaging and sorting of 3-D cultures and organoid bodies on the COPAS VISION
Authors: Francis Smet, Rock Pulak, Tom Mullins
Union Biometrica, Aalst, Belgium. Union Biometrica, Holliston, MA
Large particle flow cytometers from Union Biometrica provide automation for the analysis and dispensing of cell clusters. Cells growing in clusters communicate with each other and behave differently than cells grown as monolayers or in suspension. Research using stem cell clusters, organoids, tumor spheres and other types of 3D cultures are important biological systems for the discovery of signals responsible for normal development as well as the abnormal disease state of solid tumors. There is enormous interest in studying how cells grow, divide and differentiate in a more natural context provided by these 3D cell culture models. Many cell types will naturally form cell clusters when given the opportunity. Using this research approach allows for cell-cell interactions to occur and provides biological insights otherwise missed when studying flat sheets of cells growing on plastic surfaces or as cells grow in isolation. Furthermore, there are many methods that enable the introduction of genes for fluorescent proteins expressed from promoters of interest. Researchers can use these to track different cell types and identify when a cell or group of cells has transitioned to a different state.
COPAS VISION, our newest large particle flow cytometer, adds brightfield imaging to our large particle sorting capabilities. As with our original COPAS platform of flow cytometers it provides automated high throughput analysis and sorting of viable multicellular organisms, cell clusters, bead-based libraries and other samples that are too large or too fragile for traditional flow cytometers. COPAS VISION has expanded on these capabilities in several ways, most noteworthy is the ability to capture images of the sample objects.
The BioSorter® flow cytometer is designed to provide the flexibility for working with samples ranging in sizes from 10 to 1500 microns. This very broad range of sizes includes many interesting types of cells, cell clusters and even whole organisms. These samples can be analyzed and dispensed intact owing to the low sample pressures, low shear forces and the gentle sorting mechanism. For more detail and to see a video on BioSorter go to: http://www.unionbio.com/biosorter/
Union Biometrica has also developed an autosampler, LP Sampler™, which automatically loads samples from multiwell plates to the BioSorter for analysis and resorting. It is ideal for objects ranging from 10 to 1500 microns in diameter. It is capable of gently aspirating those samples from multiwell plates or a petri dish and delivering them intact to Union Biometrica’s BioSorter, COPAS FP and VAST BioImager™ systems. It has the flexibility to handle a variety of standard or deep well plates including 24, 96 and 384.
The COPAS FP platform is designed for the measurement of objects ranging in size from 20–1500 microns (diameter). Each of the four COPAS FP models features a fixed, specially engineered fluidic path, flow cell and optics optimized for a specific subset of that size range to provide maximum speed, accuracy, resolution and throughput, depending on the application. Instruments can dispense objects either into bulk receptacles or multiwell plates. For more detail and to see a video on COPAS FP go to: http://www.unionbio.com/copas/
The conference will take place at the Konzerthaus Freiburg, Germany from May 5-7, 2021. More information regarding the event can be found at: DECHEMA | 3D Cell Culture 2021
Not attending the meeting? Email our technical team at email@example.com for a personalized discussion or to arrange a demonstration.
About Union Biometrica, Inc.
Large Particle Flow Cytometry for Gentle Sorting -- Union Biometrica offers tools for high throughput research of bead based assays, stem cells and cell clusters (iPS cells and embryoid bodies as well as other types of large cells / cell clusters) plus model organisms (early stages of zebrafish, C.elegans, Drosophila embryos and larvae, sea urchin embryos, Xenopus eggs and embryos, Daphnia, various marine larvae and zooplankton). We provide Large Particle Flow Cytometers (COPAS and BioSorter) for objects that are too large / too fragile for traditional cytometers and offer an alternative to manual sorting. These systems analyze and sort objects based on size and fluorescent parameters. Automating this process offers increased speed, sensitivity, quantification, and repeatability of experiments.
Peer reviewed journal papers referencing our COPAS and BioSorter instruments can be viewed using the links below.
- D. rerio (zebrafish) - Flow Cytometry Publications - D. rerio (zebrafish)
- C. elegans (roundworms) - Flow Cytometry Publications - C. elegans (roundworm)
- D. melanogaster (fruit fly) – Flow Cytometry Publications - D. melanogaster (fruit fly)
- Cell and Cell Clusters - Flow Cytometry Publications - Stem Cells and Cell Clusters
- Fungi - Flow Cytometry Publications - Fungi
Imaging Tool for Zebrafish Research -- Union Biometrica, Inc. offers the VAST BioImager, for automated imaging of 2-7 dpf zebrafish larvae. Replacing the tedium of manual sample positioning, the VAST BioImager automatically loads each larva into a capillary, positions it laterally, and then rotates it in the field of view of the on-board camera for organ level imaging. Alternately the VAST can be mounted on a high resolution upright microscope for high content bright field or fluorescent imaging at the cellular level. Our LP Sampler can be added to the system to gently aspirate larvae from multi-well plates of many different formats such as 24-, 96-, 384-well and deliver them one at a time to VAST for imaging. This allows for increased throughput of high content assays and screens.
Publications related to the VAST BioImager can be view at:
- VAST - D. rerio (zebrafish) - VAST Publications - D. rerio (zebrafish)