Flow Cytometry Publications - Stem Cells and Cell Clusters

Automation for analysis and handling of cell clusters, tumor spheres and organoid bodies

Pulak et al.
February 03, 2018

Analysis and sorting of cell clusters generated in Corning microcavity flasks. 

Comparison of Total Fluorescence in Cell Clusters versus Fluorescence of the Individual Cells in the Cluster

October 23, 2017

Endothelin-1 mediates natriuresis but not polyuria during vitamin D-induced acute hypercalcaemia

Tokonami et al.
March 27, 2017
First published: 23 March 2017, DOI: 10.1113/JP273610

Fluid shear stress increases transepithelial transport of Ca2+ in ciliated distal convoluted and connecting tubule cells

Mohammed et al.
January 18, 2017
Published online before print January 18, 2017, doi: 10.1096/fj.201600687RRR, May 2017, The FASEB Journal vol. 31 no. 5 1796-1806

Urinary ß-galactosidase stimulates Ca2+ transport by stabilizing TRPV5 at the plasma membrane

Leunissen et al
January 07, 2016
Glycobiology, Volume 26, Issue 5, 1 May 2016, Pages 472–481, , Published: 07 January 2016

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  • Protein Phosphatase 1 Inhibitor-1 Deficiency Reduces Phosphorylation of Renal NaCl Cotransporter and Causes Arterial Hypotension.

    Nicolas Picard,*† Katja Trompf,* Chao-Ling Yang,‡ R. Lance Miller,§ Monique Carrel,*Dominique Loffing-Cueni,* Robert A. Fenton,|| David H. Ellison,‡ and Johannes Loffing*
    April 01, 2014
    J Am Soc Nephrol 25: 511-522, 2014. doi: 10.1681/ASN.2012121202

    *Institute of Anatomy, University of Zurch, Zurich, Switzerland †Paris Cardiovascular Research Center, Institut National de la Santé et de la Recherche Médicale Unit 970, Université Paris-Descartes, Paris, France; ‡Department of Medicine, Oregon Health and Science University, Portland, Oregon; §National Heart Lung Blood Institute, National Institutes of Health, Bethesda, Maryland; and || Department of Biomedicine, Aarhus, University, Aarhus, Denmark


    ISSCR 2013
    Wanek, Paul¹, Smet, Francis², Haupt, Simone³, Peitz, Michael³, Bongaarts, Rico², Oliver Brüstle³, Pulak, Rock4, Zenke, Martin¹
    June 13, 2013

    1) Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, RWTH Aachen University, Aachen, Germany, 2) Union Biometrica, Geel, Belgium, 3) Institute of Reconstructive Neurobiology, University of Bonn, Bonn, Germany, 4) Union Biometrica, Holliston, MA, USA

    Flow-based pipeline for systematic modulation and analysis of 3D tumor microenvironments.

    Li CY, Wood DK, Huang JH, Bhatia SN.
    April 23, 2013
    Lab Chip. 2013 Apr 23;13(10):1969-78. doi: 10.1039/c3lc41300d. Epub 2013 Apr 5.

    Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.


    Markadieu N, San-Cristobal P, Nair A, Lenssen E, Tudpor K, van Zeeland F, Bindels RJ, Hoenderop JG.
    July 03, 2012
    Am J Physiol Renal Physiol. 2012 Jul 3.

    Radboud University Nijmegen Medical Centre.

    Automated analysis and sorting of Human Induced Pluripotent Stem cells (HIPS) clusters using large particle Flow Cytometry

    ISSCR 2012
    Wanek, Paul², Smet, Francis¹, Peitz, Michael³, Bongaarts, Rico¹, Pulak, Rock4, Zenke, Martin²
    June 14, 2012

    1) Union Biometrica, Geel, Belgium, 2) Institute for Biomedical Engineering, Universitätsklinikum Aachen, RWTH, Aachen, Germany, 3) Institute of Reconstructive Neurobiology, University of Bonn, Bonn, Germany, 4) Union Biometrica, Holliston, MA, USA

    Automated neurosphere sorting and plating by the COPAS large particle sorter is a suitable method for high-throughput 3D in vitro applications

    ISSCR 2012
    Gassmann K.², Smet F.¹, Baumann J.², Giersiefer S.², Schreiber T.², Schuwald J.², R.Bongaarts², Fritsche E.²
    June 14, 2012

    2) Leibniz Research Institute for Environmental Medicine, Department Molecular Toxicology, Duesseldorf, Germany. 1) Union Biometrica, Geel, Belgium.

    Automated neurosphere sorting and plating by the COPAS large particle sorter is a suitable method for high-throughput 3D in vitro applications

    Gassmann K, Baumann J, Giersiefer S, Schuwald J, Schreiber T, Merk HF, Fritsche E.
    May 02, 2012; Toxicology in Vitro Volume 26, Issue 6, September 2012, Pages 993–1000

    Leibniz Research Institute for Environmental Medicine, Department Molecular Toxicology, Auf'm Hennekamp 50, 40225 Duesseldorf, Germany.

    Functional assessment of automatically sorted pancreatic islets using large particle flow cytometry.

    Steffen A, Ludwig B, Krautz C, Bornstein S, Solimena M.
    September 01, 2011
    Islets. 2011 Sep 1;3(5):267-70. Epub 2011 Sep 1.

    Department of Medicine III; Technische Universität Dresden; Dresden, Germany.

    Microbial encapsulation in monodisperse hydrogel microspheres enables fast and sensitive phenotypic analyses using flow cytometers

    Lidia Delgado¹, Gloria Jurado², Gema Galayo², Elena Ogalla², Lourdes Moreno³, Juan C Rodríguez-Aguilera4, Ángel Cebolla5, Carolina Sousa³, María Flores² and Sebastián Chávez¹
    February 28, 2011
    Conference Presentation at: La Société Française de Microbiologie et l’Association Française de Cytométrie

    1) Department of Genetics, Universidad de Sevilla, Seville, Spain. 2) Ingeniatrics Tecnologías SL, Spain. 3) Department of Microbiology and Parasitology, Universidad de Sevilla, Seville, Spain. 4) Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Seville, Spain. 5) Biomedal SL, Spain

    {gamma}-Adducin Stimulates the Thiazide-sensitive NaCl Cotransporter.

    Dimke H, San-Cristobal P, de Graaf M, Lenders JW, Deinum J, Hoenderop JG, Bindels RJ.
    December 16, 2010
    J Am Soc Nephrol. 2010 Dec 16.

    *Department of Physiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

    [SA-PO2763] y-Adducin Functions as a Novel Regulator of the Thiazide-Sensitive NaCl-Cotransporter

    Renal Week 2010
    Henrik Dimke, Pedro San Cristobal, Mark J. J. De Graaf, Jacob (Jaap) Deinum, Jacques W. M. Lenders, Joost G. Hoenderop, Rene J. Bindels.
    November 20, 2010

    [F-PO1585] Distal Convoluted Tubule (DCT) Specific Transcripts Identified Using DCT Large Scale Sampling

    Renal Week 2010
    Nicolas Picard, R. Lance Miller, Johannes Loffing
    November 19, 2010

    Transgenic Mice: Beyond the Knockout.

    Miller RL.
    November 10, 2010
    Am J Physiol Renal Physiol. 2010 Nov 10.

    1) National Institutes of Health.

    Tissue engineered tumor models

    M Ingram, GB Techy, BR Ward, SA Imam, R Atkinson, H Ho, CR Taylor
    August 01, 2010
    Biotechnic & Histochemistry, August 2010, Vol. 85, No. 4 : Pages 213-229

    Serum-free scaled up expansion and differentiation of murine embryonic stem cells to osteoblasts in suspension bioreactors.

    Alfred R, Gareau T, Krawetz R, Rancourt D, Kallos MS.
    August 01, 2010
    Biotechnol Bioeng. 2010 Aug 1;106(5):829-40.

    Pharmaceutical Production Research Facility (PPRF), Schulich School of Engineering, University of Calgary, 2500 University Drive N.W., Calgary, Alberta, Canada.

    Reduction of diffusion barriers in isolated rat islets improves survival, but not insulin secretion or transplantation outcome

    S Janette Williams,¹ Han-Hung Huang,¹ Karen Kover,³ Wayne Moore,³ Cory Berkland,² Milind Singh,² Irina V Smirnova,¹ Ronal MacGregor,4 and Lisa Stehno-Bittel¹
    April 01, 2010
    Organogenesis. 2010 Apr–Jun; 6(2): 115–124.

    1Department of Physical Therapy and Rehabilitation Science; University of Kansas Medical Center; Kansas City, KS USA

    4Department of Anatomy and Cell Biology; University of Kansas Medical Center; Kansas City, KS USA

    2Department of Pharmeceutical Chemistry; University of Kansas; Lawrence, KS USA

    3Endocrinology Section; Mercy Hospitals and Clinics; Kansas City, MO USA

    Primary DCT Cell Culture Using Large Scale DCT Sampling from Mouse Kidneys

    Katja Trompf,1 Nicolas Picard,1 Titia Woudenberg,2 Lance Miller,3 Rene J. Bindels,2 Hoenderop Joost,2 Johannes Loffing.1
    November 06, 2009
    Poster session: J Am Soc Nephrol 20: 2009 ; F-P01162

    1 Anatomy Inst., Univ.of Zurich, Switzerland; 2 Dpt of Physiology, Radboud Univ. Nijmegen MedicalCentre, Nijmegen, Netherlands; 3 Dpt of Pediatrics, Div. of Nephrology, School of Medicine, Univ. of Utah, Salt Lake City, UT.

    Novel method for high-throughput colony PCR screening in nanoliter-reactors

    Marcel Walser¹, Rene Pellaux¹, Andreas Meyer¹, Matthias Bechtold¹, Herve Vanderschuren², Richard Reinhardt³, Joseph Magyar4, Sven Panke¹ and Martin Held¹,*
    February 26, 2009
    Nucl. Acids Res. (2009) 37 (8): e57/1-e57/8

    1) ETH Zurich, Institute of Process Engineering, BioProcess Laboratory (BPL), 2) ETH Zurich, Institute of Plant Science, Plant Biotechnology, Zurich, Switzerland, 3) Max Planck Institute for Molecular Genetics, Analytics & Computing, Berlin, Germany and 4) Harlan Laboratories Ltd., Environmental Safety and Metabolism, Itingen, Switzerland

    Differentiation of COPAS-sorted non-endocrine pancreatic cells into insulin-positive cells in the mouse.

    Kikugawa R, Katsuta H, Akashi T, Yatoh S, Weir GC, Sharma A, Bonner-Weir S
    January 30, 2009
    Diabetologia. 2009 Jan 30. [Epub ahead of print: DOI 10.1007/s00125-009-1260-8]

    Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, 1 Joslin Place, Boston, MA, 02215, USA.

    Impact of Islet Size on Graft Function

    28th Workshop of the AIDPIT Study Group;  3rd European Diabetes Technology and Transplantation Meeting (EuDTT) – 2009, January 25-27
    Lisa Stehno-Bittel, PhD.
    January 25, 2009

    1) University of Kansas Medical Center, Great Plain Diabetes Institute

    Large Scale Sampling of Renal Distal Convoluted Tubules (DCT) by Complex Object Parametric Analysis and Sorting (COPAS)

    Renal Week 2008: American Society of Nephrology (ASN) Annual Meeting, November 04 - 09, 2008; Philadelphia, Pennsylvania
    Nicolas Picard* , Hannah Monyer, Beat Schwaller, R. Lance Miller, Johannes Loffing
    November 04, 2008

    Inst. of Anatomy, Univ. of Zurich, Zurich, Switzerland; Department of Clinical Neurobiology, Univ. of Heidelberg, Heidelberg, Germany; Unit of Anatomy, Dpt of Medicine, Univ. of Fribourg, Fribourg, Switzerland; Department of Pediatrics, Division of Nephrology, School of Medicine, Univ. of Utah, Salt Lake City, UT

    Poster session: J Am Soc Nephrol 19: 2008 ; SA-PO2135


    The renal DCT is important for the renal control of Na and K homeostasis. It also contributes to renal acid secretion and calcium and magnesium reabsorption. DCT function is difficult to study as the DCT is a rather short and difficult to identify nephron portion. Its hidden localization in the renal cortical labyrinth makes it also almost inaccessible to direct functional measurements. Recently, the group of R.L. Miller applied large-particle based flow cytometry (Complex Object Parametric Analysis and Sorting COPAS) (AJP Renal Physiol. 2006 Jul.) for large-scale sampling of collecting ducts (CD) using a transgenic mouse expressing GFP in CD. We used the same COPAS technique with a transgenic mouse expressing EGFP in the DCT in order to obtain large quantities of DCTs. Optimization of the COPAS sorting technique allows us to obtain up to 15000 fluorescent DCTs from one single mouse in less than three hours. Microscopical analysis revealed that almost all sorted tubules exhibit green fluorescence. Only occasionally some non-fluorescent tubule segments were attached. Western blot analysis confirmed the significant enrichment of the DCT-specific thiazide sensitive NaCl cotransporter in the sorted samples. Marker proteins for other nephron segments (i.e. NapiIIa - proximal tubule, NKCC2 thick ascending limb, AQP2 connecting tubule/collecting duct) were not detectable. Thus, COPAS allows rapid isolation of rather pure DCT preparations. These preparations will allow us now to study regulatory pathways involved in the DCT functions in vivo and to develop DCT cell culture for further functional testing in vitro.

    Acute antibody-mediated complement activation mediates lysis of pancreatic islets cells and may cause tissue loss in clinical islet transplantation.

    Tjernberg J, Ekdahl KN, Lambris JD, Korsgren O, Nilsson B.
    April 27, 2008
    Transplantation. 2008 Apr 27;85(8):1193-9.

    Department of Oncology, Rudbeck Laboratory, Uppsala, Sweden.

    American Journal of Physiology: Renal Physiology, Automated Method for the Isolation of Collecting Ducts

    R. Lance Miller1, Ping Zhang1, Tong Chen2, Andreas Rohrwasser2, and Raoul D. Nelson1*
    February 07, 2006

    1) Department of Pediatrics, Division of Nephrology, University of Utah, School of Medicine, Salt Lake City, UT, USA. 2) Department of Human Genetics, University of Utah, School of Medicine, Salt Lake City, UT, USA.

    Validation of Large Particle Flow Cytometry for the Analysis and Sorting of Intact Pancreatic Islets.

    Fernandez, Luis A.1; Hatch, Eric W.1; Armann, Barbara2; Odorico, Jon S.1; Hullett, Debra A.1; Sollinger, Hans W.1; Hanson, Matthew S.1
    September 27, 2005
    Transplantation. 80(6):729-737, September 27, 2005.

    1) Department of Surgery, University of Wisconsin-Madison, Madison, WI. 2) Department of Surgery, University of Leipzig, Germany

    Multicellular Cytometric Analyses and Sorting on Stem Cell Clusters and Embryonic Stem Cell Clusters (Poster)

    3rd Annual ISSCR Meeting 2005, June 23 - 25
    Tak Hung, Ph.D.1, Szczepan Baran, V.M.D.2, Carol Ware, Ph.D.2, Rock Pulak, Ph.D.1
    June 23, 2005

    1) Union Biometrica Inc., Holliston, MA 01746 USA; 2) Department of Comparative Medicine, University of Washington, Seattle, WA, USA

    Large Particle Flow Cytometry as a New Automated Method of Islet Yield Determination (Poster PS-031)

    International Pancreas and Islet Transplant Association (IPITA) 2005 Meeting, May 4-7, 2005
    M. S. Hanson1, E. W. Hatch1, B. Armann1, D. A. Hullett1, J. S. Odorico1, H. W. Sollinger1, L. A. Fernandez1
    May 04, 2005

    1Surgery, University of Wisconsin-Madison, Madison, WI, USA

    Rapid and Sensitive Assessment of Human Islet Viability by Multiparameter Flow Cytometry on Intact and Dissociated Islets (Platform Session OP-139)

    International Pancreas and Islet Transplant Association (IPITA) 2005 Meeting, May 4-7, 2005
    M. S. Hanson1, B. Armann1, E. W. Hatch1, D. A. Hullett1, J. S. Odorico1, H. W. Sollinger1, L. A. Fernandez1
    May 04, 2005

    1Surgery, University of Wisconsin-Madison, Madison, WI, USA

    Flow cytometry for isolation and optical analysis of embryoid bodies from cultured mouse embryonic stem cells.

    2nd Annual International Society of Stem Cell Researchers Meeting, June 10-13, 2004
    Dr. H. Bohlen1, Dr. S Schwengberg1, R. Bongaarts2, Dr. R. Pulak3
    June 10, 2004

    1) Axiogenesis, Joseph-Stelzmann-Str. 50, Cologne, Germany, 2) Union Biometrica -Cipalstraat 3, Geel, Belgium, 3)Union Biometrica Inc., Holliston, MA 01746 USA

    Automated fluorescence-activated collecting duct isolation for genomics and proteomics # 913.1.

    Experimental Biology 2005, April 2 - 6, 2005
    R. Lance Miller, Ping Zhang, Raoul D. Nelson.

    Pediatrics, University of Utah, 30 North 1900 East, SOM 4R312, Salt Lake City, Utah, 84108


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