Publications

Flow Cytometry Publications - C. elegans (roundworm)

Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans

Maria Doitsidou, Sophie Jarriault, Richard J. Poole
October 01, 2016
Genetics October 1, 2016 vol. 204 no. 2 451-474; DOI: 10.1534/genetics.115.186197

Selection on a Subunit of the NURF Chromatin Remodeler Modifies Life History Traits in a Domesticated Strain of Caenorhabditis elegans.

Large EE, Xu W, Zhao Y, Brady SC, Long L, Butcher RA, et al.
July 28, 2016
PLoS Genet. 2016 Jul 28;12(7):e1006219. doi: 10.1371/journal.pgen.1006219. eCollection 2016.

An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline.

Frøkjær-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ.
July 14, 2016
Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. Epub 2016 Jun 30.

Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes

Kevin Lebrigand, Le D. He, Nishant Thakur, Marie-Jeanne Arguel, Jolanta Polanowska, Bernard Henrissat, Eric Record, Ghislaine Magdelenat, Valérie Barbe,Sylvain Raffaele, Pascal Barbry, Jonathan J. Ewbank
May 06, 2016
Published: May 6, 2016 • http://dx.doi.org/10.1371/journal.pgen.1006017

A quantitative genome-wide RNAi screen in C. elegans for antifungal innate immunity genes

Olivier Zugasti1,4, Nishant Thakurr1, Jérôme Belougne1, Barbara Squiban, C.1,3, Léopold Kurz1, 4, Julien Soulé1,5 , Shizue Omi1, Laurent Tichit2, Nathalie Pujol1 and Jonathan J. Ewbank1
April 29, 2016
Zugasti et al. BMC Biology (2016) 14:35 DOI 10.1186/s12915-016-0256-3

1Centre d’Immunologie de Marseille-Luminy, Aix Marseille Université UM2,Inserm, U1104, CNRS UMR7280, 13288 Marseille, France. 2Institut de Mathématiques de Marseille, Aix Marseille Université, I2M Centrale Marseille,CNRS UMR 7373, 13453 Marseille, France. 3Present address: Section of Hematology/Oncology, Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. 4Present address: Institut de Biologie du Développement de Marseille, CNRS, UMR6216, Case 907, Marseille, France. 5Present address: Institut de Genomique Fonctionnelle, 141,rue de la Cardonille, 34094 Montpellier Cedex 05, France.

The MADD-3 LAMMER Kinase Interacts with a p38 MAP Kinase Pathway to Regulate the Display of the EVA-1 Guidance Receptor in Caenorhabditis elegans

Serena A. D’Souza 1, 2, 3, Luckshika Rajendran1, 2, Rachel Bagg1, 2, Louis Barbier 1, 2, Derek M. van Pel1, 2, Houtan Moshiri1, 2, Peter J. Roy1, 2, 3
April 28, 2016
PLoS Genet 12(4): e1006010. doi:10.1371/journal. pgen.1006010

1 Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada, 2 The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada, 3 The Collaborative Programme in Developmental Biology, University of Toronto, Toronto, Ontario, Canada
 

Mitochondrial Stress Induces Chromatin Reorganization to Promote Longevity and UPRmt.

Ye Tian, Gilberto Garcia, Qian Bian, Kristan K. Steffen, Larry Joe, Suzanne Wolff, Barbara J. Meyer, Andrew Dillin.
April 28, 2016
3. Cell, 2016; Apr 28. pii: S0092-8674(16)30402-0. DOI: 10.1016/j.cell.2016.04.011 [Epub ahead of print]

Gait-specific adaptation of locomotor activity in response to dietary restriction in Caenorhabditis elegans.

Lüersen K1 , Faust U1 , Gottschling DC1 , Döring F2 .
July 15, 2015
J Exp Biol. 2014 Jul 15;217(Pt 14):2480-8. doi: 10.1242/jeb.099382. Epub 2014 May 6.

1Department of Molecular Prevention, Institute of Human Nutrition and Food Science, Christian-Albrechts-University of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, Germany.

2Department of Molecular Prevention, Institute of Human Nutrition and Food Science, Christian-Albrechts-University of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, Germany.

The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum

Martin Gamerdinger¹, Marie Anne Hanebuth¹, Tancred Frickey², Elke Deuerling¹
April 10, 2015
Science 348, 201 (2015); DOI: 10.1126/science.aaa5335

 1Department of Biology, Institute of Molecular Microbiology, University of Konstanz, 78457 Konstanz, Germany.

2Department of Biology, Applied Bioinformatics Laboratory, University of Konstanz, 78457 Konstanz, Germany.

 

The ribonucleotidyl transferase USIP-1 acts with SART3 to promote U6 snRNA recycling

Stefan Ruëgger¹, Takashi S. Miki¹, Daniel Hess¹ and Helge Großhans¹
March 09, 2015
Nucl. Acids Res. (2015)doi: 10.1093/nar/gkv196First published online: March 9, 2015

 1Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland

The DREAM complex promotes gene body H2A.Z for target repression

Latorre I¹, Chesney MA¹, Garrigues JM, Stempor P¹, Appert A¹, Francesconi M, Strome S, Ahringer J¹
March 01, 2015
Genes Dev. 2015 Mar 1;29(5):495-500. doi: 10.1101/gad.255810.114.

1The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom

 

 

Genome-wide screen identifies a novel p97/CDC-48-dependent pathway regulating ER-stress-induced gene transcription

Esther Marza¹,², Saïd Taouji¹,², Kim Barroso¹,², Anne-Aurélie Raymond², Léo Guignard², Marc Bonneu², Néstor Pallares-Lupon¹,², Jean-William Dupuy², Martin E Fernandez-Zapico, Jean Rosenbaum², Francesca Palladino, Denis Dupuy²,and Eric Chevet*,¹,²
February 01, 2015
DOI 10.15252/embr.201439123 | Published online 04.02.2015 EMBO reports (2015) embr.201439123

1Team “Endoplasmic Reticulum stress and cancer”, INSERM, UMR1053, Bordeaux, France

2University of Bordeaux, Bordeaux, France eric.chevet@inserm.fr

 

Insect-Derived Cecropins Display Activity against Acinetobacter baumannii in a Whole-Animal High-Throughput Caenorhabditis elegans Model.

Jayamani E¹, Rajamuthiah R¹, Larkins-Ford J, Fuchs BB¹, Conery AL, Vilcinskas A, Ausubel FM, Mylonakis E4.
January 12, 2015
Antimicrob Agents Chemother. 2015 Mar;59(3):1728-37. doi: 10.1128/AAC.04198-14. Epub 2015 Jan 12.

1Division of Infectious Diseases, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, Rhode Island, USA Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.

DAF-16/FOXO and EGL-27/GATA promote developmental growth in response to persistent somatic DNA damage

Michael M. Mueller ¹, ², Laia Castells-Roca ¹, ², Vipin Babu ¹, ², Maria A. Ermolaeva ¹, ², Roman-Ulrich Müller ², Peter Frommolt², Ashley B. Williams ¹, ², Sebastian Greiss ², Jennifer I. Schneider ¹, ², Thomas Benzing ², Bernhard Schermer ², & Björn Schumacher ¹, ²
November 24, 2014
Nature Cell Biology 16, 1168–1179 (2014) doi:10.1038/ncb3071

1Institute for Genome Stability in Ageing and Disease, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 26 50931 Cologne, Germany

2Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD) Research Center and Systems Biology of Ageing Cologne, University of Cologne, Joseph-Stelzmann-Str. 26 50931 Cologne, Germany

 

Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shape

Piper Reid Hunta,, Zachary Keltnera, Xiugong Gaoa, Steven J. Oldenburg, Priyanka Bushanaa, Nicholas Olejnika, Robert L. Sprandoa
November 05, 2014
www.elsevier.com/locate/toxrep

aCenter for Food Safety and Applied Nutrition, FDA, Laurel, MD, United States

 

COPASutils: An R Package for Reading, Processing, and Visualizing Data from COPAS Large-Particle Flow Cytometers

Tyler C. Shimko, Erik C. Andersen
October 20, 2014
PLoS ONE 9(10): e111090. doi:10.1371/journal.pone.0111090

Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, United States of America

Activation of a G protein-coupled receptor by its endogenous ligand triggers the innate immune response of Caenorhabditis elegans.

Zugasti O¹, Bose N Squiban B, Belougne J¹, Kurz CL, Schroeder FC, Pujol N¹, Ewbank JJ¹
September 15, 2014
Nat Immunol. 2014 Sep;15(9):833-8. doi: 10.1038/ni.2957. Epub 2014 Aug 3.

11] Centre d'Immunologie de Marseille-Luminy, UM2 Aix-Marseille Université, Case 906, Marseille, France. [2] INSERM, U1104, 13288 Marseille, France. [3] CNRS, UMR7280, Marseille, France.

Backbone-independent nucleic acid binding by splicing factor SUP-12 reveals key aspects of molecular recognition

Samir Amrane, Karine Rebora, Ilyass Zniber, Denis Dupuy & Cameron D Mackereth
September 03, 2014
Nature Communications 5, Article number: 4595 doi:10.1038/ncomms5595

Institut Européen de Chimie et Biologie, IECB, Univ. Bordeaux, 2 rue Robert Escarpit, F-33607 Pessac, France

Inserm, U869, ARNA Laboratory, 146 rue Léo Saignat, F-33076 Bordeaux, France

ICeE: An Interface for C. elegans Experiments

Montañana F, Julien RA, Vaglio P, Matthews LR, Tichit L, Ewbank JJ.
August 15, 2014
Worm 2014; 3:e32160; http://dx.doi.org/10.4161/worm.32160

A high-content assay for identifying small molecules that reprogram C. elegans germ cell fate.

Benson JA¹, Cummings EE¹, O'Reilly LP¹, Lee MH, Pak SC¹.
August 01, 2014
Methods. 2014 Aug 1;68(3):529-535. doi: 10.1016/j.ymeth.2014.05.011. Epub 2014 Jun 2.

1Department of Pediatrics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Avenue, Pittsburgh, PA 15224, USA.

Genome evolution and plasticity of Serratia marcescens, an important multidrug resistant nosocomial pathogen

Atsushi Iguchi1, Yutaka Nagaya, Elizabeth Pradel, Tadasuke Ooka2, Yoshitoshi Ogura2,3, Keisuke Katsura3, Ken Kurokawa, Kenshiro Oshima, Masahira Hattori, Julian Parkhill, Mohamed Sebaihia, Sarah Coulthurst, Naomasa Gotoh, Nicholas R. Thomson, Jonathan J. Ewbank, Tetsuya Hayashi,2, 3
July 28, 2014
Genome Biology and Evolution Advance Access published July 28, 2014 doi:10.1093/gbe/evu160

1 Interdisciplinary Research Organization, University of Miyazaki, Miyazaki, Japan

2Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan

  3Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan

A pair of RNA-binding proteins controls networks of splicing events contributing to specialization of neural celltypes.

Norris AD1, Gao S, Norris ML1, Ray D, Ramani AK, Fraser AG, Morris Q, Hughes TR, Zhen M, Calarco JA1
June 19, 2014
Mol Cell. 2014 Jun 19;54(6):946-59. doi: 10.1016/j.molcel.2014.05.004. Epub 2014 Jun 5.

·         1FAS Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.

·       

A genome-wide RNAi screen identifies potential drug targets in a C. elegans model of a1-antitrypsin deficiency.

O'Reilly LP¹, Long OS¹, Cobanoglu MC, Benson JA, Luke CJ¹, Miedel MT¹, Hale P¹, Perlmutter DH¹, Bahar I, Silverman GA¹, Pak SC¹.
May 16, 2014
Hum Mol Genet. 2014 May 16. pii: ddu236. [Epub ahead of print]

·         1Department of Pediatrics, Cell Biology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC and Magee-Womens Hospital Research Institute, 4401 Penn Avenue, Pittsburgh, PA 15224, USA.

·       

Control of Metazoan Heme Homeostasis by a Conserved Multidrug Resistance Protein.

Korolnek T¹, Zhang J¹, Beardsley S¹, Scheffer GL, Hamza I¹
May 14, 2014
Cell Metab. 2014 May 14. pii: S1550-4131(14)00168-5. doi: 10.1016/j.cmet.2014.03.030. [Epub ahead of print]

·         1Department of Animal and Avian Sciences and Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.

·       

Progressive Degeneration of Dopaminergic Neurons through TRP Channel-Induced Cell Death.

Nagarajan A¹, Ning Y, Reisner K, Buraei Z, Larsen JP, Hobert O, Doitsidou M.
April 23, 2014
J Neurosci. 2014 Apr 23;34(17):5738-46. doi: 10.1523/JNEUROSCI.4540-13.2014.

1Center for Organelle Research, University of Stavanger, 4036 Stavanger, Norway, Norwegian Center for Movement Disorders, Stavanger University Hospital, 4011 Stavanger, Norway

The C. elegans lifespan assay toolkit.

Amrit FR¹, Ratnappan R¹, Keith SA¹, Ghazi A².
April 13, 2014
Methods. 2014 Apr 13. pii: S1046-2023(14)00146-7. doi: 10.1016/j.ymeth.2014.04.002. [Epub ahead of print]

1Department of Pediatrics, University of Pittsburgh School of Medicine, 7129 Rangos Research Centre, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15224, United States.
2Department of Pediatrics, University of Pittsburgh School of Medicine, 7129 Rangos Research Centre, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15224, United States. Electronic address: arjumand.ghazi@chp.edu.

Comparative toxicity of silver nanoparticles on oxidative stress and DNA damage in the nematode,Caenorhabditis elegans.

Ahn JM¹, Eom HJ¹, Yang X², Meyer JN², Choi J¹.
April 09, 2014
Chemosphere. 2014 Apr 9. pii: S0045-6535(14)00169-6. doi: 10.1016/j.chemosphere.2014.01.078. [Epub ahead of print]

·         1School of Environmental Engineering, University of Seoul, Seoul 130-743, Republic of Korea; Graduate School of Energy and Environmental system Engineering, University of Seoul, Seoul 130-743, Republic of Korea.

·         2Nicholas School of the Environment and Center for the Environmental Implications of Nanotechnology, Duke University, Durham, NC, USA.

·        

 

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

Kwame Twumasi-Boateng¹, Maureen Berg¹, Michael Shapira¹
March 21, 2014
JOVE 3/21/2014, Issue 85; doi: 10.3791/51090

1Department of Integrative Biology, University of California, Berkeley

Defects in the C. elegans acyl-CoA Synthase, acs-3, and Nuclear Hormone Receptor, nhr-25, Cause Sensitivity to Distinct, but Overlapping Stresses.

Ward JD¹, Mullaney B², Schiller BJ¹, He le D³, Petnic SE¹, Couillault C³, Pujol N³, Bernal TU¹, Van Gilst MR4, Ashrafi K², Ewbank JJ³, Yamamoto KR¹.
March 20, 2014
PLoS One. 2014 Mar 20;9(3):e92552. doi: 10.1371/journal.pone.0092552. eCollection 2014.

1) Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, United States of America 2) Department of Physiology, University of California San Francisco, San Francisco, California, United States of America 3) Centre d'Immunologie de Marseille-Luminy (CIML), UM2 Aix-Marseille Université, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Marseille, France; Centre National de la Recherche Scientifique (CNRS), UMR7280, Marseille, France 4) Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.

Silver Nanoparticle Behavior, Uptake, and Toxicity in Caenorhabditis elegans: Effects of Natural Organic Matter.

Yang X¹, Jiang C, Hsu-Kim H, Badireddy AR, Dykstra M, Wiesner M, Hinton DE, Meyer JN.
March 18, 2014
Environ Sci Technol. 2014 Mar 18;48(6):3486-95. doi: 10.1021/es404444n. Epub 2014 Mar 10.

1) Nicholas School of the Environment and ‡Department of Civil and Environmental Engineering, Center for the Environmental Implications of Nanotechnology, Duke University , Durham, North Carolina 27708-0328, United States.

High-Throughput Screening for Novel Anti-Infectives Using a C. elegans Pathogenesis Model.

Conery AL¹, Larkins-Ford J, Ausubel FM, Kirienko NV.
March 14, 2014
Curr Protoc Chem Biol. 2014 Mar 14;6(1):25-37. doi: 10.1002/9780470559277.ch130160.

1) Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts; Department of Genetics, Harvard Medical School, Boston, Massachusetts.

A comparative study into alterations of coenzyme Q redox status in ageing pigs, mice, and worms.

Onur S¹, Niklowitz P, Fischer A, Metges CC, Grune T, Menke T, Rimbach G, Döring F.
February 27, 2014
Biofactors. 2014 Feb 27. doi: 10.1002/biof.1160. [Epub ahead of print]

1) Division of Molecular Prevention, Institute of Human Nutrition and Food Science, Christian Albrechts University Kiel, Kiel, Germany.

A Variant in the Neuropeptide Receptor npr-1 is a Major Determinant of Caenorhabditis elegans Growth and Physiology

Erik C. Andersen¹, Joshua S. Bloom², Justin P. Gerke³, Leonid Kruglyak4
February 27, 2014
DOI: 10.1371/journal.pgen.1004156

1) Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America 2) Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America 3) Departments of Human Genetics and Biological Chemistry, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America 4) Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America

PRDE-1 is a nuclear factor essential for the biogenesis of Ruby motif-dependent piRNAs in C. elegans

Eva-Maria Weick¹, Peter Sarkies¹, Nicola Silva², Ron A. Chen¹, Sylviane M.M. Moss¹, Amy C. Cording¹, Julie Ahringer¹, Enrique Martinez-Perez² and Eric A. Miska¹
February 27, 2014
doi: 10.1101/gad.238105.114 Genes & Dev. 2014. 28: 783-796

1) Wellcome Trust Cancer Research UK Gurdon Institute, Department of Genetics, University of Cambridge, Cambridge CB2 1QN, United Kingdom; 2) MRC Clinical Sciences Centre, Imperial College Faculty of Medicine, London W12 0NN, United Kingdom

The dsRBP and inactive editor ADR-1 utilizes dsRNA binding to regulate A-to-I RNA editing across the C. elegans transcriptome.

Washburn MC¹, Kakaradov B, Sundararaman B, Wheeler E, Hoon S, Yeo GW, Hundley HA2
February 27, 2014
Cell Rep. 2014 Feb 27;6(4):599-607. doi: 10.1016/j.celrep.2014.01.011. Epub 2014 Feb 6.

1Department of Biology, Indiana University, Bloomington, IN 47405, USA. Bioinformatics and Systems Biology Program, University of California, San Diego, La Jolla, CA 92093-0419, USA; 

2Medical Sciences Program, Indiana University, Bloomington, IN 47405, USA. Electronic address: hahundle@indiana.edu

 

Evaluation of The Mitochondrial Redox Status in Caenorhabditis elegans: An Assay Based on The COPAS Biosort Flow Cytometer

HU Mingxi¹,², XU Tao¹, CHEN Chang¹
February 20, 2014
Acta Biophysica Sinica 2014, 30(2) 110-116 DOI: 10.3724/SP.J.1260.2014.40021 ISSN: 1000-6737 CN: 11-1992/Q

1Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

2University of the Chinese Academy of Sciences, Beijing 100049, China

S-Adenosyl methionine synthetase 1 limits fat storage in Caenorhabditis elegans

Madeleine Ehmke, Katharina Luthe, Ralf Schnabel, and Frank Döring
February 08, 2014
Genes Nutr. Mar 2014; 9(2): 386. Published online Feb 8, 2014. doi: 10.1007/s12263-014-0386-6

Department of Molecular Prevention, Institute of Human Nutrition and Food Sciences, University of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, Germany Department of Developmental Genetics, Institute of Genetics, TU Braunschweig, Spielmannstr. 7, 38106 Brunswick, Germany

Comparative RNAi Screens in C. elegans and C. briggsae Reveal the Impact of Developmental System Drift on Gene Function

Adrian J. Verster¹,² Arun K. Ramani,¹,²2 Sheldon J. McKay,³ and Andrew G. Fraser¹,²,*
February 06, 2014
Published online Feb 6, 2014. doi: 10.1371/journal.pgen.1004077

1) The Donnelly Centre, University of Toronto, Toronto, Ontario, Canada 2) Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada 3) Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America 4) University of California Davis, United States of America

 

 

Fluphenazine Reduces Proteotoxicity in C. elegans and Mammalian Models of Alpha-1-Antitrypsin Deficiency

Jie Li¹, Stephen C. Pak¹, Linda P. O’Reilly¹, Joshua A. Benson¹, Yan Wang¹, Tunda Hidvegi¹, Pamela Hale¹, Christine Dippold¹, Michael Ewing¹, Gary A. Silverman¹, David H. Perlmutter¹
January 31, 2014
Published: January 31, 2014 • DOI: 10.1371/journal.pone.0087260

1Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America ; Children's Hospital of Pittsburgh of University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, United States of America.

Forward and reverse mutagenesis in C. elegans.

Lena M. Kutscher¹, and Shai Shaham¹
January 17, 2014
Kutscher L. M., Shaham S. Forward and reverse mutagenesis in C. elegans (January 17, 2014), WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.167.1, http://www.wormbook.org.

1) Laboratory of Developmental Genetics, The Rockefeller University, New York NY 10065, USA

Sulfidation of silver nanoparticles: natural antidote to their toxicity.

Levard C, Hotze EM, Colman BP, Dale AL, Truong L, Yang XY, Bone AJ, Brown GE Jr, Tanguay RL, Di Giulio RT, Bernhardt ES, Meyer JN, Wiesner MR, Lowry GV.
December 03, 2013
Environ Sci Technol. 2013 Dec 3;47(23):13440-8. doi: 10.1021/es403527n. Epub 2013 Nov 15.

RNA-binding protein GLD-1/quaking genetically interacts with the mir-35 and the let-7 miRNA pathways in Caenorhabditis elegans

Alper Akay 1,2, Ashley Craig1, Nicolas Lehrbach2, Mark Larance1, Ehsan Pourkarimi1, Jane E. Wright3, Angus Lamond1, Eric Miska2, and Anton Gartner1
November 20, 2013
Open Biol. 2013 3, 130151. http://dx.doi.org/10.1098/rsob.130151

1)Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, UK 2)Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK 3)Friedrich Miescher Institute for Biomedical Research, Basel 4002, Switzerland

Modular Control of Glutamatergic Neuronal Identity in C. elegans by Distinct Homeodomain Proteins.

Serrano-Saiz E, Poole RJ, Felton T, Zhang F, De La Cruz ED, Hobert O.
October 24, 2013
Cell. 2013 Oct 24;155(3):659-73. doi: 10.1016/j.cell.2013.09.052. Epub 2013 Oct 24.

Department of Biochemistry and Molecular Biophysics, HHMI, Columbia University Medical Center, New York, NY 10032, USA. Electronic address: es2754@columbia.edu.

Comparative toxicogenomic responses of mercuric and methyl-mercury.

Matthew K McElwee1, Lindsey A Ho2, Jeff W Chou3, 4, Marjolein V Smith2, and Jonathan H Freedman1,*
October 11, 2013
McElwee et al. BMC Genomics 2013, 14:698. http://www.biomedcentral.com/1471-2164/14/698

* Corresponding author: Jonathan H Freedman freedma1@niehs.nih.gov Author Affiliations 1)Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, NIH, 111 T.W Alexander Drive, Research Triangle Park, P.O. Box 12233, 27709 Durham, NC, USA 2)SRA International, Inc., Durham, NC, USA 3)Microarray and Genome Informatics Group, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, Durham, NC, USA 4)Current address: Department of Biostatistical Sciences, Wake Forest University School of Medicine, Medical Center Boulevard, 27157 Winston-Salem, NC, USA

Comparison of the toxicity of fluoridation compounds in the nematode C. elegans.

Rice JR, Boyd WA, Chandra D, Smith MV, Den Besten PK, Freedman JH.
September 16, 2013
Environ Toxicol Chem. 2013 Sep 16. doi: 10.1002/etc.2394.

Biomolecular Screening Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA.

A Pro-Cathepsin L Mutant Is a Luminal Substrate for Endoplasmic-Reticulum-Associated Degradation in C.elegans

Mark T. Miedel, Nathan J. Graf, Kate E. Stephen, Olivia S. Long, Stephen C. Pak, David H. Perlmutter, Gary A. Silverman, Cliff J. Luke
July 02, 2013
PLoS ONE 7(7): e40145. doi:10.1371/journal.pone.0040145

Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children’s Hospital of Pittsburgh of UPMC and Magee-Womens Hospital of UPMC, Pittsburgh, Pennsylvania, United States of America

EGL-13/SoxD specifies distinct O2 and CO2 sensory neuron fates in Caenorhabditis elegans.

Gramstrup Petersen J, Rojo Romanos T, Juozaityte V, Redo Riveiro A, Hums I, Traunmüller L, Zimmer M, Pocock R.
May 09, 2013
PLoS Genet. 2013 May;9(5):e1003511. doi: 10.1371/journal.pgen.1003511. Epub 2013 May 9.

Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark.

Active transcriptomic and proteomic reprogramming in the C. elegans nucleotide excision repair mutant xpa-1.

Arczewska, K.D., Tomazella, G.G., Lindvall, J.M., Kassahun, H., Maglioni, S., Torgovnick, A., Henriksson, J., Matilainen, O., Marquis, B.J., Babaie, E., Holmberg, C.I., Bürglin, T.R., Ventura, N., Thiede, B., Nilsen, H.
May 01, 2013
Nucl. Acids Res., 41, 5368-5381.

piRNAs can trigger a multigenerational epigenetic memory in the germline of C. elegans.

Ashe A, Sapetschnig A, Weick EM, Mitchell J, Bagijn MP, Cording AC, Doebley AL, Goldstein LD, Lehrbach NJ, Le Pen J, Pintacuda G, Sakaguchi A, Sarkies P, Ahmed S, Miska EA.
July 06, 2012
Cell. 2012 Jul 6;150(1):88-99. Epub 2012 Jun 25.

Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.

Toxicity ranking of heavy metals with screening method using adult Caenorhabditis elegans and propidium iodide replicates toxicity ranking in rat

Piper Reid Hunt *, Nicholas Olejnik ¹, Robert L. Sprando ²,
July 04, 2012
Food Chem Toxicol. 2012 Sep;50(9):3280-90. Epub 2012 Jul 4.

United States Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Division of Toxicology,8301 Muirkirk Road, Laurel, MD 20708, United States

* Corresponding author. Address: Mod. 1, Room 2002, 8301 Muirkirk Road,Laurel, MD 20708-2476, USA. Tel.: +1 301 210 8897.1) Address: Mod. 1, Room 2011, 8301 Muirkirk Road, Laurel, MD 20708-2476, USA. Tel.: +1 301 210 6375. 2) Address: Mod. 1, Room 2017, 8301 Muirkirk Road, Laurel, MD 20708-2476, USA. Tel.: +1 301 210 6329.

E-mail addresses: Piper.Hunt@fda.hhs.gov (P.R. Hunt), Nicholas.Olejnik@fda.hhs.gov (N. Olejnik), Sprando@fda.hhs.gov (R.L. Sprando).

Caenorhabditis elegans as a model in developmental toxicology.

Boyd WA, Smith MV, Freedman JH.
May 31, 2012
Methods Mol Biol. 2012;889:15-24.

Biomolecular Screening Branch, National Toxicology Program, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.

Daf-2 signaling modifies mutant SOD1 toxicity in C. elegans

Boccitto M, Lamitina T, Kalb RG.
March 20, 2012
PLoS One. 2012;7(3):e33494. Epub 2012 Mar 20.

Department of Pediatrics, Division of Neurology, Children's Hospital of Philadelphia Research Institute, Philadelphia, Pennsylvania, United States of America. boccitto@mail.med.upenn.edu

 

 

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Barbara Squiban, Jérôme Belougne, Jonathan Ewbank, Olivier Zugasti
February 27, 2012
http://www.jove.com/video/3448

Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée

Topologically conserved residues direct heme transport in HRG-1-related proteins.

Yuan X, Protchenko O, Philpott CC, Hamza I.
February 10, 2012
J Biol Chem. 2012 Feb 10;287(7):4914-24. doi: 10.1074/jbc.M111.326785. Epub 2011 Dec 15.

Department of Animal & Avian Sciences and Department of Cell Biology & Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA.

A Genome-Wide Collection of Mos1 Transposon InsertionMutants for the C. elegans Research Community

Elodie Vallin¹,²·, Joseph Gallagher³·, Laure Granger¹,², Edwige Martin¹,², Jérôme Belougne4,5,6, Julien Maurizio4,5,6, Yohann Duverger4,5,6¤a, Sarah Scaglione4,5,6¤b, Caroline Borrel¹,², Elisabeth Cortier¹,²,Karima Abouzid¹,²¤c, Maite´ Carre-Pierrat¹,², 7¤d, Kathrin Gieseler¹,², Laurent Ségalat¹,²", Patricia E.Kuwabara³", Jonathan J. Ewbank4,5,6*"
February 08, 2012
PLoS ONE 7(2): e30482. doi:10.1371/journal.pone.0030482

 1)Centre de Génétique et de Physiologie Moléculaires et Cellulaires, CNRS UMR 5534, Campus de la Doua, Villeurbanne, France, 2) Université Claude Bernard Lyon 1,Villeurbanne, France, 3) School of Biochemistry, University of Bristol, Bristol, United Kingdom, 4) Centre d’Immunologie de Marseille-Luminy, Aix-Marseille University,Marseille, France, 5) INSERM, U1104, Marseille, France, 6) CNRS, UMR7280, Marseille, France, 7) Plateforme ‘‘Biologie de Caenorhabditis elegans’’, CNRS UMS3421, Campus dela Doua, Villeurbanne, France

* E-mail: ewbank@ciml.univ-mrs.fr

. These authors contributed equally to this work.
" These authors also contributed equally to this work.
¤a Current address: UPR 9043 Laboratoire de Chimie Bactérienne, CNRS, Marseille, France
¤b Current address: UPR3081 Instabilité du Génome et Cancérogénèse, CNRS, 13009 Marseille, France
¤c Current address: VECT-HORUS, Faculté de Médecine Nord, CS80011, Marseille, France
¤d Current address: Plateforme ‘‘Biologie de Caenorhabditis elegans’’, CNRS-UBCL UMS 3421, Campus de la Doua, Villeurbanne, France

 

The Majority of Animal Genes Are Required for Wild-Type Fitness

Arun K. Ramani,¹,² Tungalag Chuluunbaatar,¹,² Adrian J. Verster,¹,² Hong Na,¹,² Victoria Vu,¹,² Nadège Pelte,¹,² Nattha Wannissorn,¹,² Alan Jiao,¹,² and Andrew G. Fraser,¹,²,*
January 19, 2012
DOI 10.1016/j.cell.2012.01.019; Cell. 2012 Feb 17;148(4):792-802.

1) The Donnelly Centre, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada 2) Department of Molecular Genetics, University of Toronto, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada

*Correspondence: andyfraser.utoronto@gmail.com

 

HIF-1 Regulates Iron Homeostasis in Caenorhabditis elegans by Activation and Inhibition of Genes Involved in Iron Uptake and Storage

Steven Joshua Romney, Ben S. Newman, Colin Thacker, Elizabeth A. Leibold
December 15, 2011
PLoS Genet 7(12): e1002394. doi:10.1371/journal.pgen.1002394

Steven Joshua Romney, Elizabeth A. Leibold
Department of Medicine, University of Utah, Salt Lake City, Utah, United States of America
Ben S. Newman
University of Washington, Seattle, Washington, United States of America
Colin Thacker
Department of Biology, University of Utah, Salt Lake City, Utah, United States of America
Elizabeth A. Leibold
Department of Oncological Sciences, University of Utah, Salt Lake City, Utah, United States of America

An intercellular heme-trafficking protein delivers maternal heme to the embryo during development in C. elegans.

Chen C, Samuel TK, Sinclair J, Dailey HA, Hamza I.
May 27, 2011
Cell. 2011 May 27;145(5):720-31. doi: 10.1016/j.cell.2011.04.025.

Department of Animal & Avian Sciences and Department of Cell Biology & Molecular Genetics, University of Maryland, College Park, MD 20742, USA.

Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans eveal fat stores using microscopy and flow cytometry approaches

Klapper M, Ehmke M, Palgunow D, Böhme M, Matthäus C, Bergner G, Dietzek B, Popp J, Döring F
March 18, 2011
J Lipid Res. 2011 Jun;52(6):1281-93. Epub 2011 Mar 18.

Institute of Human Nutrition and Food Science, Research Group Molecular Prevention, University of Kiel, Kiel, Germany.

The Caenorhabditis elegans mucin-like protein OSM-8 negatively regulates osmosensitive physiology via the transmembrane protein PTR-23.

Rohlfing AK, Miteva Y, Moronetti L, He L, Lamitina T.
January 06, 2011
PLoS Genet. 2011 Jan 6;7(1):e1001267.

Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.

Proteome changes of Caenorhabditis elegans upon a Staphylococcus aureus infection

Annelies Bogaerts*, Isabel Beets, Liesbet Temmerman, Liliane Schoofs, Peter Verleyen
January 05, 2011
Bogaerts et al. Biology Direct 2010, 5:11; http://www.biology-direct.com/content/5/1/11

Automated High-Content Live Animal Drug Screening Using C. elegans Expressing the Aggregation Prone Serpin a1-antitrypsin Z.

Sager J. Gosai¹, Joon Hyeok Kwak¹, Cliff J. Luke¹, Olivia S. Long¹, Dale E. King¹, Kevin J. Kovatch¹, Paul A. Johnston², Tong Ying Shun², John S. Lazo², David H. Perlmutter¹, Gary A. Silverman¹*, Stephen C. Pak¹*
November 01, 2010
PLoS ONE 5(11): e15460. doi:10.1371/journal.pone.0015460

1) Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC and Magee-Womens Hospital Research Institute, Pittsburgh, Pennsylvania, United States of America, 2) Department of Pharmacology and Chemical Biology, University of Pittsburgh Drug Discovery Institute, University of Pittsburgh, Pennsylvania, United States of America

 

 

Insulin-like signaling determines survival during stress via posttranscriptional mechanisms in C. elegans .

McColl G, Rogers AN, Alavez S, Hubbard AE, Melov S, Link CD, Bush AI, Kapahi P, Lithgow GJ.
September 08, 2010
Cell Metab. 2010 Sep 8;12(3):260-72.

The Mental Health Research Institute, Parkville, Victoria, Australia. gmccoll@mhri.edu.au

An antibiotic selection marker for nematode transgenesis.

Rosina Giordano-Santini¹, Stuart Milstein2,4, Nenad Svrzikapa4, Domena Tu5, Robert Johnsen2, David Baillie 5, Marc Vidal2,4 & Denis Dupuy¹
September 01, 2010
Nature America, Inc. 2010

1) Genome Regulation and Evolution, INSERM U869, Institut Européen de Chimie et Biologie, Pessac, France. 2) Center for Cancer Systems Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. 3) Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. 4) Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. 5) Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.

 

Caenorhabditis elegans as a screening tool to find active compounds and targets in rare neuromuscular diseases.

Aging, Metabolism, Stress, Pathogenesis, and Small RNAs University of Wisconsin-Madison, Madison, WI, USA, August 1-4, 2010
Maïté Carre-Pierrat, Jean Giacomotto, Jessica Cisek and Laurent Ségalat
August 01, 2010

CNRS UMR5534 – Centre de Génétique Moléculaire et Cellulaire, 16 rue Raphaël Dubois – bâtiment Mendel, Campus de La Doua – 69100 Villeurbanne - FRANCE

Optimizing analysis of high-throughput screens with the UBI Biosort

Aging, Metabolism, Stress, Pathogenesis, and Small RNA's in C. elegans, August 1-4, 2010, University of Wisconsin, Madison, WI
Frédéric Montanana¹, Jérôme Belougne¹, Aurélie Autret¹, Barbara Squiban¹, C. Leopold Kurz¹, Olivier Zugasti¹, Renaud Julien², Fabien Cavasino², Jonathan Ewbank¹
June 16, 2010

 1) Centre d'Immunologie de Marseille Luminy, Case 906, 13288 Marseille Cedex 9, France, 2) Modul-Bio, Parc Scientifique Luminy Biotech II, Case 935,13288 Marseille Cedex 9

 

A suite of MATLAB-based computational tools for automated analysis of COPAS Biosort data

Elizabeth Morton and Todd Lamitina
June 01, 2010
BioTechniques 48:xxv-xxx (The RNA World June 2010) doi 10.2144/000113427

Department of Physiology, University of Pennsylvania, Philadelphia, PA, USA

A differential proteomics study of Caenorhabditis elegans: infected with Aeromonas hydrophila.

Bogaerts A, Temmerman L, Boerjan B, Husson SJ, Schoofs L, Verleyen P.
June 01, 2010
Copyright 2010 Elsevier Ltd. All rights reserved.

Research Group of Functional Genomics and Proteomics, K.U. Leuven, Naamsestraat 59, 3000 Leuven, Belgium. annelies.bogaerts@bio.kuleuven.be

The fatty acid synthase fasn-1 acts upstream of WNK and Ste20/GCK-VI kinases to modulate anitmicrobial peptide expression in C. elegans epidermis

Kwang-Zin Lee¹,³, Marina Kniazeva 4, Min Han4, Nathalie Pujol¹,³, and Jonathan J. Ewbank¹,³
May 31, 2010
Landes Bioscience, Virulence 1:3, 113-122; May/June 2010

1) Centre d’Immunologie de Marseille-Luminy; Université de la Méditerranée; Marseille, France; 2) INSERM; U631; Marseille, France; 3) CNRS; UMR6102; Marseille, France; 4)Howard Hughes Medical Institute and Dept of MCDB; University of Colorado at Boulder; Colorado, USA

 

High-throughput screening and small animal models, where are we?

Giacomotto J, Ségalat L.
May 01, 2010
Br J Pharmacol. 2010 May;160(2):204-16.

Université Claude Bernard Lyon, Villeurbanne, France. giacomottojean@gmail.com <giacomottojean@gmail.com>

A Conserved PMK-1/p38 MAPK Is Required in Caenorhabditis elegans: Tissue-specific Immune Response to Yersinia pestis Infection*

Devin D. Bolz, Jennifer L. Tenor, and Alejandro Aballay¹
February 04, 2010
Received for publication, December 3, 2009, and in revised form, January 22, 2010 Published, JBC Papers in Press, February 4, 2010, DOI 10.1074/jbc.M109.091629

1) From the Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710

LIN-28 and the poly(U) polymerase PUP-2 regulate let-7 microRNA processing in Caenorhabditis elegans .

Lehrbach NJ, Armisen J, Lightfoot HL, Murfitt KJ, Bugaut A, Balasubramanian S, Miska EA.
October 16, 2009
Nat Struct Mol Biol. 2009 Oct;16(10):1016-20. Epub 2009 Aug 27.

Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, The Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK.

Identification of Antifungal Compounds Active against Candida albicans Using an Improved High-Throughput Caenorhabditis elegans Assay

Ikechukwu Okoli1, Jeffrey J. Coleman1, Emmanouil Tempakakis1, W. Frank An2, Edward Holson2, Florence Wagner2, Annie L. Conery3, Jonah Larkins-Ford3, Gang Wu3, Andy Stern2, Frederick M. Ausubel3, Eleftherios Mylonakis1*
September 14, 2009
PLoS ONE 4(9): e7025. doi:10.1371/journal.pone.0007025

1 Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America, 2 Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, United States of America,

 

Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans

Doitsidou, M., Flames, N., Lee, A.C., Boyanov, A. & Hobert, O.  Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, 701 W. 168th Street, New York, New York 10032, USA
August 31, 2009
Nature Methods 5, 869 - 872 (2008). Published online: 31 August 2008 | doi:10.1038/nmeth.1250

High-Throughput Screen for Novel Antimicrobials using a Whole Animal Infection Model

Terence I. Moy†,‡,§, Annie L. Conery‡,§, Jonah Larkins-Ford‡,§, Gang Wu‡,§, Ralph Mazitschek¶, Gabriele Casadei_, Kim Lewis_, Anne E. Carpenter_, and Frederick M. Ausubel†,‡,§,*
June 29, 2009
Published on June 29, 2009 on http://pubs.acs.org | doi: 10.1021/cb900084v

†Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, ‡Department of Molecular Biology, §Center for Computational and Integrative Biology, and Center for Systems Biology, Massachusetts General Hospital,Boston, Massachusetts 02114, _Department of Biology, Northeastern University, Boston, Massachusetts 02115, and_Imaging Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142

Whole-Animal High-Throughput Screens: The C. elegans Model

Eyleen J. O’Rourke , Annie L. Conery , and Terence I. Moy
June 01, 2009
P.A. Clemons et al. (eds.), Cell-Based Assays for High-Throughput Screening, Methods in Molecular Biology, Vol. 486 © Humana Press a part of Springer Science + Business Media, LLC 2009DOI: 10.1007/978-1-60327-545-3_5

Antifungal Innate Immunity in :C. elegans PKCdelta Links G Protein Signaling and a Conserved p38 MAPK Cascade.

Ziegler K, Kurz CL, Cypowyj S, Couillault C, Pophillat M, Pujol N, et al.
April 23, 2009
Cell host & microbe. 2009 Apr;5(4):341-52.

A method to rank order water soluble compounds according to their toxicity using Caenorhabditis elegans: a Complex Object Parametric Analyzer and Sorter, and axenic liquid media.

Sprando RL, Olejnik N, Cinar HN, Ferguson M.
April 01, 2009
Food Chem Toxicol. 2009 Apr;47(4):722-8. Epub 2009 Jan 8.

Division of Toxicology, United States Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Laural, MD 20708, United States. Robert.sprando@fda.hhs.gov

Neuroimmune regulation of antimicrobial peptide expression by a noncanonical TGF-beta signaling pathway in Caenorhabditis elegans epidermis.

Zugasti O and Ewbank JJ.
February 08, 2009
Nature Immunology. 2009 10:249-256.

Negative regulation of Caenorhabditis elegans epidermal damage responses by death-associated protein kinase.

Tong A, Lynn G, Ngo V, Wong D, Moseley SL, Ewbank JJ, et al.
February 01, 2009
Proceedings of the National Academy of Sciences of the United States of America. 2009 Feb;106(5):1457-61.

A C. elegans -based, whole animal, in vivo screen for the identification of antifungal compounds

Emmanouil Tampakakis, Ikechukwu Okoli & Eleftherios Mylonakis
November 20, 2008
Published online 20 November 2008; doi:10.1038/nprot.2008.193

 Division of Infectious Diseases, Massachusetts General Hospital, Gray-Jackson 504, 55 Fruit Street, Boston, Massachusetts 02114, USA.

Anti-Fungal Innate Immunity in C. elegans Is Enhanced by Evolutionary Diversification of Antimicrobial Peptides

Nathalie Pujol 1, 2, 3, #, Olivier Zugasti 1, 2, 3, #, Daniel Wong 1, 2, 3 #, Carole Couillault 1, 2, 3, C. Léopold Kurz 1, 2, 3, Hinrich Schulenburg 4, Jonathan J. Ewbank 1, 2, 3, *
July 01, 2008
PLoS Pathogens 4(7): e1000105 doi:10.1371/journal.ppat.1000105.

1 Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, Marseille, France   2 INSERM, U631, Marseille, France   3 CNRS, UMR6102, Marseille, France   4 Department of Animal Evolutionary Ecology, Zoological Institute, University of Tuebingen, Tuebingen, Germany

Distinct Innate Immune Responses to Infection and Wounding in the C. elegans Epidermis.

Pujol N, Cypowyj S, Ziegler K, Millet A, Astrain A, Goncharov A, Jin Y, Chisholm AD, Ewbank JJ.
April 08, 2008
Curr Biol. 2008 Apr 8;18(7):481-489.

Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, 13288 Marseille cedex 9, France.

Discovery of a cholecystokinin-gastrin-like signaling system in nematodes.

Janssen T, Meelkop E, Lindemans M, Verstraelen K, Husson SJ, Temmerman L, Nachman RJ, Schoofs L.
March 13, 2008
Endocrinology. 2008 Jun;149(6):2826-39. Epub 2008 Mar 13.

Effects of Genetic Mutations and Chemical Exposures on Caenorhabditis elegans Feeding: Evaluation of a Novel, High-Throughput Screening Assay

Windy A. Boyd1, Sandra J. McBride2, Jonathan H. Freedman1, 2 *
December 05, 2007
PLoS ONE 2(12): e1259 doi:10.1371/journal.pone.0001259

1 Laboratory of Molecular Toxicology, National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, United States of America   2 Nicholas School of the Environment and Earth Sciences, Duke University, Durham, North Carolina, United States of America

Abstract
Government agencies have defined a need to reduce, refine or replace current mammalian-based bioassays with testing methods that use alternative species. Invertebrate species, such as Caenorhabditis elegans, provide an attractive option because of their short life cycles, inexpensive maintenance, and high degree of evolutionary conservation with higher eukaryotes. The C. elegans pharynx is a favorable model for studying neuromuscular function, and the effects of chemicals on neuromuscular activity, i.e., feeding. Current feeding methodologies, however, are labor intensive and only semi-quantitative.

Here a high-throughput assay is described that uses flow cytometry to measure C. elegans feeding by determining the size and intestinal fluorescence of hundreds of nematodes after exposure to fluorescent-labeled microspheres. . . .

A global analysis of genetic interactions in Caenorhabditis elegans

Alexandra B Byrne*, Matthew T Weirauch, Victoria Wong*, Martina Koeva, Scott J Dixon*, Joshua M Stuart and Peter J Roy*
September 26, 2007
Journal of Biology, 2007, 6:8

Addresses: *Department of Medical Genetics and Microbiology, The Terrence Donnelly Centre for Cellular and Biomolecular Research, 160 College St, University of Toronto, Toronto, ON, M5S 3E1, Canada. Collaborative Program in Developmental Biology, University of Toronto, Toronto, ON, M5S 3E1, Canada. Department of Biomolecular Engineering, 1156 High Street, Mail Stop SOE2, University of California, Santa Cruz, CA 95064, USA.

Correspondence: Peter J Roy. Email: peter.roy@utoronto.ca; Joshua M Stuart. Email: jstuart@soe.ucsc.edu

16th International C. elegans Conference 2007 June 27 - 30

June 27, 2007

Click on the titles of the following posters to view an abstract:

Genome-scale analysis of in vivo spatiotemporal promoter activity in C. elegans.
Denis Dupuy, Nicolas Bertin, César Hidalgo, Kavitha Venkatesan, Domena Tu, David Lee, Nenad Svrzikapa, Anne-Ruxandra Carvunis, Rock Pulak, Ian Hope, John Reece-Hoyes, Rebecca Hunt-Newbury, Ryan Viveiros, William Mohler, Murat Tasan, Frederick Roth, Donald Moerman, Albert-László Barabási, David Baillie, Marc Vidal.

Genetic dissection of the transcriptional response to wounding and fungal infection.
Nathalie Pujol, C. Leopold Kurz, Sophie Cypowyj, Daniel Wong, Andrew Chisholm, Jonathan Ewbank.

A high throughput screen for novel anti-infective compounds.
Terence I. Moy, Annie L. Conery, Anne E. Carpenter, Anthony R. Ball, Kim Lewis, Frederick M. Ausubel.

Characterization of the role of peni( fr8) in innate immunity against fungal infection.
Kwang-Zin Lee, Nathalie Pujol, Andrew Chisholm, Jonathan Ewbank.

Automated drug screening and chemical genetics in C. elegans models of rare diseases.
Jean Giacomotto, Maité Carre-Pierrat, Laurent Segalat.

Isolation and characterization of C. elegans mutants deficient in PMK-1-dependent immunity.
Robert Shivers, Tristan Kooistra, Bethany Redding, Dennis H. Kim.

A Sodium: Neurotransmitter symporter Family protein required for the induction of antimicrobial peptides in C. elegans.
Katja Ziegler, Jonathan Ewbank, Nathalie Pujol.

High-throughput chemical screening using C. elegans growth and development.
Windy A. Boyd, Sandra J. McBride, Marjolein V. Smith, Grace E. Kissling, Julie R. Rice, Daniel W. Snyder, Christopher J. Portier, Jonathan H. Freedman.

Using a C. elegans feeding response assay in high-throughput chemical and genetic screening.
Windy A. Boyd, Sandra J. McBride, Jonathan H. Freedman.

Genetic dissection of Spinal Muscular Atrophy in Drosophila and C. elegans.
Jevede D. Harris, Tom Barsby, Amy K. Walker, Anne C. Hart. Gelsomino, Paolo Bazzicalupo.

Large scale genetic screens for cell fate mutants using worm sorter technology.
Vincent Bertrand, Nuria Flames, Maria Doitsidou, Richard Poole, Janine Recio, Oliver Hobert.

Semi-automated genetic screen for temperature sensitive mutations that abolish the early embryonic expression of the hox gene ceh-13.
Stephan Knierer, Adrian Streit.

The C. elegans dysferlin homolog fer-1 is expressed in muscle.
Todd Lamitina, Olga Lozynska, Tejvir Khurana.

Toward the development of a database for the storage and integration of COPAS™ BioSorter™ data.
Lisa R. Matthews, Philippe Vaglio, Jonathan Ewbank.

A French functional genomics platform.
Yohann Duverger, Sarah Scaglione, Daniel Wong, Jérôme Reboul, Jonathan Ewbank.

A semi-automated high-throughput approach to the generation of transposon insertion mutants.
Yohann Duverger, Jérôme Belougne, Sarah Scaglione, Dominique Brandli, Christophe Beclin, Jonathan Ewbank.

A green light to expression in time and space.

Mango SE.
June 25, 2007
Nat Biotechnol. 2007 Jun;25(6):645-6. No abstract available.

Genome-scale analysis of in vivo spatiotemporal promoter activity in Caenorhabditis elegans.

Denis Dupuy1,10, Nicolas Bertin1,2,10, César A Hidalgo1,3, 10, Kavitha Venkatesan1, Domena Tu4, David Lee4, Jennifer Rosenberg1, Nenad Svrzikapa1, Aurélie Blanc1, Alain Carnec1, Anne-Ruxandra Carvunis1, Rock Pulak5, Jane Shingles6, John Reece-Hoyes6, Rebecca Hunt-Newbury7, Ryan Viveiros7, William A Mohler8, Murat Tasan9, Frederick P Roth9, Christian Le Peuch2, Ian A Hope6, Robert Johnsen4, Donald G Moerman7, Albert-László Barabási1,3, David Baillie4 & Marc Vidal1
May 07, 2007
Nature Biotechnology 25, 663 - 668 (2007)

1Center for Cancer Systems Biology (CCSB), and Department of Cancer Biology, Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA. 2Centre de Recherche en Biochimie Macromole´culaire, Centre National de la Recherche Scientifique FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France. 3Center for Complex Network Research, Department of Physics, University of Notre Dame, 225 Nieuwland Science Hall, Notre Dame, Indiana 46556, USA. 4Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada. 5Union Biometrica, 84 October Hill Road, Holliston, Massachusetts 01746, USA. 6Institute of Integrative and Comparative Biology, University of Leeds, Clarendon Way, Leeds LS2 9JT, West Yorkshire, UK. 7Department of Zoology, The University of British Columbia, 6270 University Boulevard, Vancouver, British Columbia V6T 1Z4, Canada. 8Department of Genetics and Developmental Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030, USA. 9Center for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA. 10These authors contributed equally to this work. Correspondence should be addressed to M.V. (marc_vidal@dfci.harvard.edu), D.B. (baillie@sfu.ca) or A.-L.B. (alb@nd.edu).

A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans

Yohann Duverger1,2,3, Jérôme Belougne4, Sarah Scaglione1,2,3, Dominique Brandli4, Christophe Beclin4 and Jonathan J. Ewbank 1,2,3*
November 03, 2006
Nucleic Acids Research, 2006, Vol. 00, No. 00 e1–8

1Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, 13288 Marseille cedex 9, France, 2INSERM, U631, 13288 Marseille, France, 3CNRS, UMR6102, 13288 Marseille, France and 4CNRS, Institut de Biologie du Développement de Marseille-Luminy, Marseille, France.

Received September 5, 2006; Revised October 18, 2006; Accepted November 3, 2006.

Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo

Hidehito Kuroyanagi1,2, Tetsuo Kobayashi3,4, Shohei Mitani3,4, & Masatoshi Hagiwara1,2
November 01, 2006
Nature Methods 3, 909 - 915 (01 Nov 2006) Article

1School of Biomedical Science and 2Medical Research Institute, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. 3Department of Physiology, Tokyo Women’s Medical University School of Medicine, Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. 4CREST, JST, Hon-cho, Kawaguchi, Saitama 332-0012, Japan.

A small-molecule screen in C. elegans yields a new calcium channel antagonist.

Kwok TC, Ricker N, Fraser R, Chan AW, Burns, Stanley EF, McCourt P, Cutler SR, Roy PJ
May 04, 2006
Nature. 2006 May 4;441(7089):91-5.

Department of Medical Genetics and Microbiology, and The Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.

Genome-wide RNAi screening identifies protein damage as a regulator of osmoprotective gene expression.

Lamitina T, Huang CG, Strange K
April 12, 2006
Proc Natl Acad Sci U S A.

Departments of Anesthesiology and Pharmacology, Vanderbilt University, T4208 Medical  Center North, 1161 21st Avenue South, Nashville, TN 37232.

The detection, stabilization, and repair of stress-induced damage are essential requirements for cellular life. All cells respond to osmotic stress-induced water loss with increased expression of genes that mediate accumulation of organic osmolytes, solutes that function as chemical chaperones and restore osmotic homeostasis. The signals and signaling mechanisms that regulate osmoprotective gene expression in animal cells are poorly understood. Here, we show that gpdh-1 and gpdh-2, genes that mediate the accumulation of the organic osmolyte glycerol, are essential for survival of the nematode Caenorhabditis elegans during osmotic stress. Expression of GFP driven by the gpdh-1 promoter (Pgpdh-1::GFP) is detected only during hypertonic stress but is not induced by other stressors. Using Pgpdh-1::GFP expression as a phenotype, we screened approximately 16,000 genes by RNAi feeding and identified 122 that cause constitutive activation of gpdh-1 expression and glycerol accumulation. Many of these genes function to regulate protein translation and cotranslational protein folding and to target and degrade denatured proteins, suggesting that the accumulation of misfolded proteins functions as a signal to activate osmoprotective gene expression and organic osmolyte accumulation in animal cells. Consistent with this hypothesis, 73% of these protein-homeostasis genes have been shown to slow age-dependent protein aggregation in C. elegans. Because diverse environmental stressors and numerous disease states result in protein misfolding, mechanisms must exist that discriminate between osmotically induced and other forms of stress-induced protein damage. Our findings provide a foundation for understanding how these damage-selectivity mechanisms function.

PMID: 16880390 [PubMed - as supplied by publisher]

A stress-sensitive reporter predicts longevity in isogenic populations of Caenorhabditis elegans

Shane L Rea1,4, Deqing Wu1,4, James R Cypser1, James W Vaupel2 & Thomas E Johnson1,3
July 24, 2005
Nature Genetics Vol. 37, pgs. 894 - 898 (2005)

1) Institute for Behavioral Genetics, University of Colorado at Boulder, Box 447, Boulder, Colorado 80309, USA. 2) Max Planck Institute for Demographic Research, Konrad-Zuse-Strasse 1, D-18057, Rostock, Germany. 3) Department of Integrative Biology, University of Colorado at Boulder, Box 447, Boulder, Colorado 80309, USA. 4) These authors contributed equally to this work. Published online: 24 July 2005.

A non-biased, in vivo genetic screen for genes that protect against necrosis. (Poster 758A)

15th International C. elegans Meeting 2005, June 25 - 29
Wenying Zhang, Monica Driscoll
June 25, 2005

Dept Mol Biol & Biochem, Rutgers Univ, Piscataway, NJ.

Multi-parameter axial profiling of transgenic C. elegans expressing fluorescent proteins from various cell-specific, tissue specific and developmentally regulated promoters. (Poster 1172A)

15th International C. elegans Meeting 2005, June 25 - 29
Bo Wang1, Julia Thompson1, Yanping Zhang2, Michael Herman2, Mariya Lomakina1, Bruce Holcombe1, Rock Pulak1
June 25, 2005

1) Union Biometrica, Holliston, MA; 2) Division of Biology, Kansas State University, Manhattan, Kansas.

A French functional genomics platform. (Poster 1294C)

15th International C. elegans Meeting 2005, June 25 - 29
Aurélie Blanc1, Yohann Duverger1 Jérôme Reboul2, Daniel Wong1, Jonathan Ewbank1
June 25, 2005

1) Centre d’Immunologie de Marseille-Luminy, INSERM/CNRS/Université de la Méditerranée, Marseille, France; 2) INSERM UMR 599, Institut Paoli Calmette, Marseille, France.

The C. elegans Localizome Project: a beginning. (Plenary Session 212)

15th International C. elegans Meeting 2005, June 25 - 29
Denis Dupuy1, Nicolas Bertin1, Qianru Li1, Alain Carnec1, Jennifer Rosenberg1, Rock Pulak2, Jane Shingles3, John Reece-Hoyes3, Domena Tu4, David Lee4, Rebecca Newbury5, Ryan Viveiros5, William A. Mohler6, Ian A. Hope3, Don Moerman5, Robert Johnsen4, David Baillie4, Marc Vidal1
June 25, 2005

1) Center for Cancer Systems Biology (CCSB)/Dana-Farber Cancer Institute/Harvard Medical school, Boston, MA; 2) Union Biometrica, Holliston, MA; 3) University of Leeds, Leeds, UK; 4) Department Molecular Biology and Biochemistry, Simon Fraser University, Vancouver, BC; 5) Department of Zoology, The University of British Columbia, BC; 6) Dept. of Genetics and Developmental Biology University of Connecticut Health Center, Farmington, CT.

Identification of new innate immunity genes. (Parallel Session 294)

15th International C. elegans Meeting 2005, June 25 - 29
Anne Millet, Nathalie Pujol, Jonathan Ewbank.
June 25, 2005

Centre d’Immunologie de Marseille-Luminy, INSERM/CNRS/Univ. de la Méditerranée, Marseille, France.

Stochastic Effects Make a Big Difference in How Long You Will Live (If You Are a Worm). (Plenary session 311)

15th International C. elegans Meeting 2005, June 25 - 29
Thomas E. Johnson, Shane Rea, Deqing Wu, Jim Cypser
June 25, 2005

Inst Behavioral Genetics, University of Colorado, Boulder, CO.

A novel method of quantifying C. elegans feeding. (Poster 408B)

15th International C. elegans Meeting 2005, June 25 - 29
Windy A. Boyd, Sandra J. McBride, Jonathan H. Freedman
June 25, 2005

Nicholas School of the Environment and Earth Sciences, Duke University, Durham, NC.

Development of medium-throughput toxicity screens using C. elegans. (Poster 409C)

15th International C. elegans Meeting 2005, June 25 - 29
Windy A. Boyd, Sandra J. McBride, Jonathan H. Freedman
June 25, 2005

Nicholas School of the Environment and Earth Sciences, Duke University, Durham, NC.

Studies of Interactions between PAR Proteins. (Poster 1105C)

15th International C. elegans Meeting 2005, June 25 - 29
Jin Li1, Tak-Jun Hung2, Donato Aceto1, Shinya Aono3, Melissa Beers1, Kenneth J Kemphues1
June 25, 2005

1) Dept Molecular Biol & Genetics, Cornell Univ, Ithaca, NY; 2) Union Biometrica, Holliston, MA; 3) Department of Morphoregulaltion, Institute for Frontier Medical Sciences, Kyoto Univ, Kyoto, Japan.

Genetic analysis of osmotically regulated gene expression in C. elegans

Experimental Biology 2005, April 2 - 6, 2005
S. Todd Lamitina, Kevin Strange
April 02, 2005

Department of Anesthesiology, Vanderbilt University, 1161 21st Avenue South, MCN T4208, Nashville, TN, 37232.

XNP-1/ATR-X acts with RB, HP1 and the NuRD complex during larval development in C. elegans

H. Carlos Cardosoa, Carole Couillaultb, Cecile Mignon-Ravixa, Anne Milletb, Jonathan J. Ewbankb, Michel Fonte´sa, Nathalie Pujolb,*
February 01, 2005
Developmental Biology, Volume 278, Issue 1, Pages 49- 59

aINSERM U491, Faculte´ de Me´decine la Timone, 27, Bd Jean Moulin, 13385 Marseille Cedex 5, France bCentre d'Immunologie de Marseille Luminy, INSERM/CNRS/Universite´ de la Me´diterrane´e, Campus Luminy Case 906, 13288 Marseille Cedex 9, France.

Diversity and specificity in the interaction between Caenorhabditis elegans and the pathogen Serratia marcescens

Hinrich Schulenburg*1 and Jonathan J Ewbank2
November 22, 2004
BMC Evolutionary Biology, 4:49

1) Department of Evolutionary Biology, Institute for Animal Evolution and Ecology, Westphalian Wilhelms-University, Hüfferstr. 1, 48149 Münster, Germany and 2) Centre d'Immunologie de Marseille Luminy, INSERM/CNRS/Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France.

Published: 22 November 2004. doi:10.1186/1471-2148-4-49.

High-throughput sublethal toxicity tests using the nematode Caenorhabditis elegans.

SETAC (Soc. of Environmental Toxicology & Chemistry) 2004, Nov 14-18, 2004
Boyd, Windy1, McBride, Sandra1, Rice, Julie1, Freedman, Jonathan1
November 14, 2004

1) Duke University, Durham, NC, USA

Automated assays to study longevity in C. elegans

Maren Hertweck(1), and Ralf Baumeister
October 20, 2004
Mechanisms of Ageing and Development

Maren Hertweck(1), and Ralf Baumeister, Bio3/Bioinformatics and Molecular Genetics,  Albert-Ludwigs University of Freiburg, Sch&auml;nzlestr. 1, D-79104 Freiburg, Germany.

An Unlikely Star of Science: Jonathan Freedman Looks to Microscopic Roundworms to Document the Effects of Toxic Chemicals.

Jonathan Freedman
September 21, 2004
DukeEnvironment Magazine, Fall 2004

Long-term effects of sterol depletion in C. elegans : Sterol content of synchronized wild-type and mutant populations.

Mark Merris*, Jessica Kraeft*, G. S. Tint†.§ and John Lenard1*
August 16, 2004
Journal of Lipid Research, Volume 45 (2004)

*Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854; Department of Veterans Affairs New Jersey Health Care System, 385 Tremont Avenue, East Orange, NJ 07018; §Department of Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07101

Chapter 4: Practical applications of RNAi in C. elegans, "RNA interference: From Basic Biology to Drug Development,"

Stephens, K.E., O. Zugasti, N.J. O'Neil, P.E. Kuwabara
June 21, 2004
Cambridge University Press

Discusses the use of the COPAS BIOSORT to score RNAi phenotypes complete with illustrations and pictures. SUMMER, 2004

TLR-independent control of innate immunity in Caenorhabditis elegans by the TIR domain adaptor protein TIR-1, an ortholog of human SARM

Carole Couillault1, Nathalie Pujol1, Jérôme Reboul2, Laurence Sabatier3, Jean-François Guichou4, Yuji Kohara5 & Jonathan J Ewbank1
March 28, 2004
Nature Immunology 5, 488 - 494 (2004)

1) Centre d'Immunologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale/Centre National de la Recherche Scientifique/Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France. 2) Institut National de la Santé et de la Recherche Médicale U119, Institut Paoli Calmette, 13009 Marseille, France. 3) Institut de Biologie Moléculaire et Cellulaire, UPR 9022, Centre National de la Recherche Scientifique, 15 rue Descartes, 67084 Strasbourg Cedex, France. 4) Centre de Biochimie Structurale, Centre National de la Recherche Scientifique UMR 5048, Institut National de la Santé et de la Recherche Médicale UMR 554, Université de Montpellier 1, 29, rue de Navacelles, 34090 Montpellier Cedex, France. 5) National Institute of Genetics, Mishima 411, Japan.

Model Organisms in Drug Discovery (2003): Chapter 3: Caenorhabditis elegans Functional Genomics in Drug Discovery: Expanding Paradigms.

Titus Kaletta, Lynn Butler, Thierry Bogaert
December 31, 2003
John Wiley & Sons, Ltd

Devgen NV, Technologiepark 9, B-9052 Ghent-Zwijnaarde, Belgium

Book Editor(s): Pamela M. Carroll, Kevin Fitzgerald Copyright © 2003 John Wiley & Sons, Ltd

Published Online: 05 Jan 2005
Print ISBN: 0470848936; Online ISBN: 0470014067

A worm's life [Med Sci Paris]

Pujol, N. and Ewbank, J.J.
December 28, 2003
Med Sci (Paris) 19, 1209-17 (2003)

Centre d'Immunologie de Marseille Luminy, Cnrs UMR 6102, Inserm U. 136, Universite de la Mediterranee, Case 906, 13288 Marseille Cedex 09, France.

Use of the COPAS Biosort in a hi-throughput screen for genes that regulate lifespan.

Lunch Seminar at Drug Discovery Technology Conference, August 11-13, 2003
Douglas Crawford and Cynthia Kenyon
August 11, 2003

Department of Biochemistry and Biophysics, University of California, San Francisco.

Imaging strategies and data types for genome-wide comparison and pattern-matching of GFP expression patterns: GLO-Worm, Program #41.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
William A. Mohler
June 29, 2003

Genetics and Dev. Bio., UConn Health Center, Farmington USA.

Isolation of Long-Lived Individuals Within an Isogenic Population, Program # 145.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
Shane L. Rea, Deqing Wu, Abigail Smith, Thomas E. Johnson
June 29, 2003

Institute for Behavioral Genetics, University of Colorado at Boulder, Boulder, CO USA.

Anti-fungal innate immune defence in C. elegans, Program #287.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
Carole Couillault1, Nathalie Pujol1, Laurence Sabatier2, Jean-Francois Guichou3, Yuji Kohara4, Jonathan Ewbank1
June 29, 2003

1) CIML, Marseille, France; 2) IBMC, Strasbourg, France; 3) CBS, Montpellier, France; 4) NIG, Mishima, Japan.

GFP screens for regulators of motor neuron differentiation, Program #520A.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
Joseph D. Watson, David M. Miller
June 29, 2003

Neuroscience Program Cell and Developmental Biology, Vanderbilt University, Nashville, TN USA.

Applications of the COPAS (Complex Object Parametric Analysis and Sorting) Biosort to functional genomic studies, Program #1111A.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
Judith S. Gordon, Nigel J. O'Neil, Patricia E. Kuwabara
June 29, 2003

The Wellcome Trust Sanger Institute, Cambridge, UK.

Mos1 mutagenesis and the Union Biometrica worm sorter: complementary tools for a genetic screen for worms that resist bacterial infection, Program #314B.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
Anne Millet, Jonathan Ewbank
June 29, 2003

CIML, Marseille, FRANCE.

A high throughput screening method to detect youthful nematodes, Program #356B.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
Peter J. Schmeissner, Suzhen Guo, Laura A. Herndon, Monica Driscoll
June 29, 2003

Molecular Biology and Biochem, Rutgers University, Piscataway, NJ USA.

Measurement and analysis of stress induced responses in transgenic Caenorhabditis elegans, Program #389B.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
Rock Pulak, Britta Moellers, Julia Thompson
June 29, 2003

Union Biometrica, Holliston, MA USA.

Developing an automating worm-based screen for bacterial virulence factors using the Union Biometrica sorter, Program #312C.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
C. Lèopold Kurz, Aurèlie Blanc, Elizabeth Pradel, Jonathan Ewbank
June 29, 2003

CIML, Marseille, FRANCE.

Genetic analysis of IFT and ARPKD: Isolating osm-5 suppressors, Program #510C.

The 14th Biennial C. elegans Conference, June 29-July 3, 2003
Renee L. Engle1, Hongmin Qin2, Joel Rosenbaum2, Maureen M. Barr3
June 29, 2003

1) Laboratory of Genetics, University of Wisconsin, Madison, WI USA; 2) Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT USA; 3) School of Pharmacy, University of Wisconsin, Madison, WI, USA.

An Automationed High-Throughput Assay for Survival of the Nematode Caenorhabditis elegans

Matthew S. Gill, Anders Olsen, James N. Sampayo, and Gordon J. Lithgow
May 23, 2003
Free Radical Biology & Medicine Vol. 35, No. 6, pp. 558-565

Buck Institute, Novato, CA (USA). PII: S0891-5849(03)00328-9. SEPTEMBER, 2003

Caenorhabditis elegans: an emerging genetic model for the study of innate immunity

C. Léopold Kurz and Jonathan J. Ewbank
May 01, 2003
Nature Reviews Genetics 4, 380 -390 (2003)

Centre d'Immunologie de Marseille Luminy, INSERM/CNRS/Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France.

Measurement and analysis of stress induced responses in transgenic Caenorhabditis elegans, #L340.

American Society of Cell Biology 42nd Annual Meeting, December 14-18, 2002
R. Pulak, J. Thompson, B. Moellers
December 14, 2002

Life Sciences, Union Biometrica, Inc., Holliston, MA.

GFP screens for regulators of motor neuron differentiation, #242.

West Coast C. elegans Meeting, August 10 - 13, 2002
Joseph Watson, David M. Miller, III
August 10, 2002

Dept. of Cell and Developmental Biology, Vanderbilt University Med. Ctr., Nashville, TN

Check out my Profile! Isolation of chemotaxis defective mutants with altered str-1 expression levels using automated, high-sensitivity fluorescence Profiling, #12

West Coast C. elegans Meeting, August 10 - 13, 2002
Anthony A. Ferrante1, Britta Moellers1, Jennifer Kean1, Gregory O'Connor1, Vance Chang1, Bruce Holcombe1, Peter Van Osta2, Steven Alam1
August 10, 2002

1) Union Biometrica, Inc., 35 Medford St, Holliston, MA 01746, 2) Union Biometrica, GMBH, Cipalstraat 3, B-2440, Geel, Belgium

The application of scale space and the spatial color model in microscopy.

Microscience 2002, London, July 9-11, 2002
P. Van Osta, Union Biometrica N.V., European Scientific Operations; Additional authors: K. Ver Donck*, J.M. Geusebroek**, L. Bols*, J.Geysen*, B.M. ter; Haar Romeny***
July 09, 2002

* Union Biometrica N.V., European Scientific Operations, Geel, Belgium. ** ISIS, Faculty of Science, University of Amsterdam, Amsterdam, the Netherlands. *** TU Eindhoven, Faculty Biomedical Technology, Biomedical Imageprocessing, Den Dolech, Eindhoven, the Netherlands

The Multi-Mode Mosaic framework for automated microscopy and analysis.

Microscience 2002, London, July 9-11, 2002
P. Van Osta, Union Biometrica N.V., European Scientific Operations, Additional authors: K. Ver Donck*, J.M. Geusebroek**, L. Bols*, J.Geysen*
July 09, 2002

* Union Biometrica N.V., European Scientific Operations. ** ISIS, Faculty of Science, University of Amsterdam, Amsterdam, the Netherlands

Suppressors of gon-1, #626415

Midwest Worm Meeting, June 28-30, 2002
Dan Hesselson1, Judith Kimble2, 3
June 28, 2002

1) Department of Genetics, University of Wisconsin-Madison, Madison, WI; 2) Department of Biochemistry, University of Wisconsin-Madison, Madison, WI; 3) Howard Hughes Medical Institute, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI

Isolation of chemotaxis defective mutants with altered str-1 expression levels using automated, high-sensitivity fluorescence profiling, #496373.

Midwest Worm Meeting, June 28-30, 2002
Anthony A. Ferrante, Britta Moellers, Jennifer Kean, Gregory O'Connor, Vance Chang, Bruce Holcombe, Steve Alam, Peter Van Osta
June 28, 2002

Union Biometrica, Inc., Holliston, MA.

Application of linear scale space and the spatial color model in microscopy

Joint Microscopy Meeting, Lille, France, June 24-28, 2002
P. Van Osta*, K. Ver Donck*, L. Bols*, J.Geysen*, J.M. Geusebroek**, B.M. ter Haar Romeny***
June 24, 2002

* Union Biometrica N.V., European Scientific Operations, Cipalstraat 3, B-2440 Geel, Belgium, ** Intelligent Sensory Information Systems, Faculty of Science, UvA,  Amsterdam, The Netherlands, *** BioMedische Technologie, Technische Universiteit Eindhoven, Eindhoven, The Netherlands.

Post-embryonic Developmental Expression Chronograms: a new functional genomics data type generated using a nematode fluorescence sorting system, #178.

2002 East Coast Worm Meeting, June 14-16, 2002
William A. Mohler1, Rock Pulak2, Anthony Ferrante2
June 14, 2002

1) University of Connecticut Health Center, Farmington CT, 2) Union Biometrica, Holliston MA

Check out my Profile! Isolation of mutants with altered str-1 expression levels using automated, high-sensitivity fluorescence profiling, #112.

2002 East Coast Worm Meeting, June 14-16, 2002
Anthony A. Ferrante, Britta Moellers, Vance Chang, Bruce Holcombe, Steve Alam
June 14, 2002

Union Biometrica, Inc., Holliston, MA

Multi-parameter & Dual Color Fluorescence Analysis and Flow Sorting of C. elegans, #199.

2002 East Coast Worm Meeting, June 14-16, 2002
Rock Pulak, Jen Kean, Britta Moellers
June 14, 2002

Union Biometrica, Inc., Holliston, MA

Enhanced Analytical Performance of the C. elegans Flow Sorter COPAS BIOSORT: Automated Re-analysis of Populations in Multi-well Plates and Reading of Axially Distributed Positional Fluorescent Signals, #32.

European Worm Meeting, May 18-21, 2002
Johan Geysen*, Steve Alam, Anthony Ferrante, Peter Kalutkewitz, Peter Van Osta*, Susan Zusman
May 18, 2002

Union Biometrica Inc. & European Scientific Operations(*)

Enhanced One-step Nematode Recognition on micrographs of Living C. elegans Cultures in 384-well Plates using Linear Scale Space Mathematics.

European Worm Meeting, May 18-21, 2002
Peter Van Osta*, Kris Ver Donck*, Jan-Mark Geusebroek**, Luc Bols* and Johan Geysen*
May 18, 2002

*Union Biometrica, European Scientific Operations, Geel, Belgium; **Intelligent Sensory Information Systems, UvA, Amsterdam, the Netherlands.

Multi-parametric & dual color fluorescent analysis and flow sorting of C. elegans.

European Worm Meeting, May 18-21, 2002
Rico Bongaarts*, Luc Bols*, Johan Geysen*, Anthony Ferrante**, Brian Dell'Orfano**, Susan Zusman**
May 18, 2002

*Union Biometrica, European Scientific Operations, Geel, Belgium; **Union Biometrica, Holliston, MA, USA.

Fully Automated Instrumentation for Analysis in C elegans, #1089.

2001 International Worm Meeting, June 22-26, 2001
Rock Pulak, Peter Kalutkiewicz, Anthony Ferrante, Greg O'Connor, Jennifer Kean, Ralph Clover
June 22, 2001

Union Biometrica, Inc., Holliston, MA.

Application of the COPAS (Complex Object Parametric Analysis and Sorting) dispenser to functional genomic studies, #1096.

2001 International Worm Meeting, June 22-26, 2001
GL Bell, NJ O'Neil, A Coulson, PE Kuwabara
June 22, 2001

The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK

Automated Sorting of C. elegans Muv Mutants According to Pseudovulva Number, #1097.

2001 International Worm Meeting, June 22-26, 2001
Anthony A. Ferrante, Peter Kalutkiewicz, Steve Alam, Russell Gershman, W. Peter Hansen
June 22, 2001

Union Biometrica, Inc., Holliston, MA

Boy Is My Bursa Red: Automated Detection and Sorting of Fluorescent Stained C. elegans Males From a Mixed Population, #89.

2000 East Coast Worm Meeting, June 9-11, 2000
A Ferrante, L Thibodeau, G O'Connor, WP Hansen
June 09, 2000

Union Biometrica, Inc. (Holliston, MA).

Adapting a manual clonal screen to semi-automation, #858.

1999 International Worm Meeting, June 2 - 6, 1999
BT Tsung1, AA Ferrante2, WP Hansen2, PB Krauledat2, C Johnson3, CP Hunter1
June 02, 1999

1) MCB, Harvard University, Cambridge, MA, 02138., 2) Union Biometrica, 19 Ward St., Holliston, MA 01746, 3) Axys, Nemapharm Group, South San Francisco, CA

Automated sorting and dispensing of C. elegans to wells of microtiter plates, #443.

1999 International Worm Meeting, June 2 - 6, 1999
CD Johnson1, R Clover1, B Reardon1, PB Krauledat2, AA Ferrante2, WP Hansen2
June 02, 1999

1) Axys Pharmaceuticals, NemaPharm Group, South San Francisco, CA, 2) Union Biometrica, Inc., Holliston, MA.

Commentary: Classical genetics goes high-tech

David S Fay
doi:10.1038/nmeth1008-863

 

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