Measured Parameters

Five parameters are recorded for each object of interest:

  • Optical density of the object (optical extinction, EXT)
  • Axial length (Time of Flight, TOF) of the object
  • Simultaneous detection of up to three colors of fluorescence

COPAS FP instruments allow for multiple fluorescence excitation and emission wavelengths. In the standard configuration, the instrument has fluorescence detectors for the green, yellow and/or red regions of the spectrum to cover GFP, YFP and RFP fluorescent proteins, as well as numerous other commercially available fluorophores.

Sorting rates vary with the concentration of the sample and percentage of the total sample that is being dispensed, but can range up to 100,000 events per hour.