Union Biometrica, Inc. to present at the 54th Annual Meeting of the Society of Nematologists in East Lansing, MI
Join us for the following poster presentation which will be located in position # 63:
DEVELOPING AUTOMATED METHODS FOR ANALYSIS AND SORTING OF GLOBODERA PALLIDA CYSTS, EGGS, AND JUVENILES USING THE COPAS 1000 FP LARGE PARTICLE FLOW CYTOMETER
Malinouski Mikalai1, Rowley J2, Dandurand LM2
1- Union Biometrica Inc. 84 October Hill Rd, Holliston, MA 01746; 2 - Department of Plant, Soil and Entomological Sciences, University of Idaho 875 Perimeter Drive MS 2339, Moscow, ID 83844
Potato cyst nematodes, such as Globodera pallida, are economically important plant parasites and threaten global food security. G. pallida is also a valuable model organism to study the biology of cyst nematodes. Handling large quantities of G. pallida for screening purposes is a labor intensive process. Here we report the development of an automated method for the purification of G. pallida cysts from soil samples using a COPAS 1000 large-particle flow cytometer. Using this method, we purified 72 cysts in approximately 5 minutes, and confirmed purity by microscopic observation. We were also able to isolate, analyze, and sort various populations of G. pallida eggs. Two distinct populations of eggs were identified based on red auto-fluorescence (AF) signal. A 15-fold difference in fluorescence intensity (ex. 488nm, em. 615nm) was noted between the two populations. Eggs exhibiting low red AF were verified as living and high red AF were verified as dead using Meldola’s Blue staining. Further, the COPAS 1000 flow cytometer was used to accurately sort user-specified numbers of juvenile worms to wells of 96-well plates. We have developed a method for the high-throughput analysis and sorting of different populations of potato nematode cysts, eggs, and juvenile worms on the basis of size, optical density, and fluorescent parameters. We were able to identify and purify cysts from soil/root particles, distinguish viable and dead eggs, and sort precise numbers of juvenile worms into desired collection receptacles.
The meeting will be held at Michigan State University from July 19-24, 2015. More information about the meeting can be found at: http://www.nematologists.org/son_annual_meeting.php
Not attending the meeting? To learn more about our family of COPAS products go to: http://www.unionbio.com/copas/
About Union Biometrica, Inc.
Large Particle Flow Cytometry for Gentle Sorting of Model Organisms - Union Biometrica offers tools for high throughput research of model organisms such as C.elegans, Drosophila embryos and larvae, early stages of zebrafish, sea urchin embryos, Xenopus eggs and embryos, Daphnia, various marine larvae and zooplankton. We provide Large Particle Flow Cytometers (COPAS and BioSorter) for objects that are too large / too fragile for traditional cytometers and offer an alternative to manual sorting. These systems analyze and sort objects based on size and fluorescent parameters. Automating this process offers increased speed, sensitivity, quantification, and repeatability of experiments.