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Union Biometrica, Inc. to exhibit at Plant Biology 2016 in Austin, TX
Working with fragile plant protoplasts has long been a challenge. Union Biometrica has recently demonstrated high speed, gentle sorting of protoplasts. We have successfully used our instruments to sort intact plant protoplasts and encapsulated protoplasts. We will be available at the meeting to discuss some new applications of the BioSorter, including the use of plant cell clusters (calli), pollen and Arabidopsis thaliana seeds. These samples can be analyzed and dispensed intact owing to the low sample pressures, low shear forces and the gentle sorting mechanism.
The COPAS FP platform is designed for the measurement of objects ranging in size from 20–1500 microns (diameter). Each of the four COPAS FP models features a fixed, specially engineered fluidic path, flow cell and optics optimized for a specific subset of that size range to provide maximum speed, accuracy, resolution and throughput, depending on the application. Instruments can dispense objects either into bulk receptacles or multiwell plates.
The Large Particle Sampler (LP Sampler) is the only automated sample introduction system designed specifically for gentle handling of large, fragile objects up to 1500 microns in diameter. It is capable of gently aspirating those samples from multiwell plates or a petri dish and delivering them intact and viable to the BioSorter for COPAS FP systems.
Additional information regarding the meeting is available at: http://plantbiology.aspb.org/
About Union Biometrica, Inc.
Large Particle Flow Cytometry for Gentle Sorting -- Union Biometrica offers tools for high throughput research of bead based assays, stem cells and cell clusters (iPS cells and embryoid bodies as well as other types of large cells / cell clusters) plus model organisms (early stages of zebrafish, C.elegans, Drosophila embryos and larvae, sea urchin embryos, Xenopus eggs and embryos, Daphnia, various marine larvae and zooplankton). We provide Large Particle Flow Cytometers (COPAS and BioSorter) for objects that are too large / too fragile for traditional cytometers and offer an alternative to manual sorting. These systems analyze and sort objects based on size and fluorescent parameters. Automating this process offers increased speed, sensitivity, quantification, and repeatability of experiments.
Peer reviewed journal papers and application notes referencing the sorting of small plant models using our BioSorter and COPAS instruments can be viewed via the links below.
• Arabidopsis: http://www.unionbio.com/publications/publication.aspx?id=36
• Protoplasts and Calli: http://www.unionbio.com/applications/notes.aspx?id=54
• Pollen: http://www.unionbio.com/applications/notes.aspx?id=22
• Fungi: http://www.unionbio.com/publications/publication.aspx?id=34