Flow cytometry can be used with various kinds of plant samples, such as pollen, certain small seeds, delicate plant protoplasts, and calli. To learn more, visit Union Biometrica, Inc. at the Plant Biology 2021 Worldwide Summit.
Working with fragile plant protoplasts has long been a challenge. Union Biometrica has recently demonstrated high speed, gentle sorting of protoplasts. We have successfully used our instruments to sort intact plant protoplasts and encapsulated protoplasts. Information will be available regarding applications of the BioSorter, including the use of plant cell clusters (calli), pollen and Arabidopsis thaliana seeds. These samples can be analyzed and dispensed intact owing to the low sample pressures, low shear forces and the gentle sorting mechanism. The BioSorter® flow cytometer is designed to provide the flexibility for working with samples ranging in sizes from 10 to 1500 microns. For more detail and to see a video on BioSorter go to: BioSorter Platform Overview | Union Biometrica
Union Biometrica has also developed an autosampler, LP Sampler™, which automatically loads samples from multiwell plates to the BioSorter for analysis and resorting. It is ideal for objects ranging from 10 to 1500 microns in diameter. It is capable of gently aspirating those samples from multiwell plates or a petri dish and delivering them intact to Union Biometrica’s BioSorter, COPAS FP and VAST BioImager™ systems. It has the flexibility to handle a variety of standard or deep well plates including 24, 96 and 384.
Our COPAS VISION large particle flow cytometer, adds brightfield imaging to our large particle sorting capabilities. As with our original COPAS platform of flow cytometers it provides automated high throughput analysis and sorting of viable multicellular organisms, cell clusters, bead-based libraries and other samples that are too large or too fragile for traditional flow cytometers. COPAS VISION has expanded on these capabilities in several ways, most noteworthy is the ability to capture images of the sample objects. For more details go to: COPAS VISION Platform Overview | Union Biometrica
The COPAS FP platform is designed for the measurement of objects ranging in size from 20–1500 microns (diameter). Each of the four COPAS FP models features a fixed, specially engineered fluidic path, flow cell and optics optimized for a specific subset of that size range to provide maximum speed, accuracy, resolution and throughput, depending on the application. Instruments can dispense objects either into bulk receptacles or multiwell plates. For more detail and to see a video on COPAS FP go to: COPAS Flow Pilot Platform Overview | Union Biometrica
More information regarding the meeting can be found at: Plant Biology 2021 Worldwide Summit | July 19-23, 2021 (aspb.org)
Not attending the meeting? Email our technical team at firstname.lastname@example.org for a personalized discussion or to arrange a demonstration in your laboratory. Virtual demonstrations are available as well.
About Union Biometrica, Inc.
Large Particle Flow Cytometry for Gentle Sorting -- Union Biometrica offers tools for high throughput research of seeds (Arabidopsis), protoplasts and calli, encapsulated cells, bead based assays, stem cells and cell clusters (iPS cells and embryoid bodies as well as other types of large cells / cell clusters) plus model organisms (early stages of zebrafish, C.elegans, Drosophila embryos and larvae, sea urchin embryos, Xenopus eggs and embryos, Daphnia, various marine larvae and zooplankton). We provide Large Particle Flow Cytometers (COPAS and BioSorter) for objects that are too large / too fragile for traditional cytometers and offer an alternative to manual sorting (5-1500 micron diameter). These systems analyze and sort objects based on size and fluorescent parameters. Automating this process offers increased speed, sensitivity, quantification, and repeatability of experiments.
Peer reviewed journal papers and application notes referencing the sorting of small plant models using our BioSorter and COPAS instruments can be viewed via the links below.
• Arabidopsis: Small Plant Models: Arabidopsis - Flow Cytometry for Large (10-1500 micron) Particles | Union Biometrica and Flow Cytometry Publications - Seeds - Flow Cytometry for Large (10-1500 micron) Particles | Union Biometrica
• Protoplasts and Calli: Small Plant Models: Protoplasts & Calli - Flow Cytometry for Large (10-1500 micron) Particles | Union Biometrica
• Pollen: Flow Cytometry Publications - Pollen - Flow Cytometry for Large (10-1500 micron) Particles | Union Biometrica
• Fungi: Flow Cytometry Publications - Fungi - Flow Cytometry for Large (10-1500 micron) Particles | Union Biometrica and Fungi and Others - Flow Cytometry for Large (10-1500 micron) Particles | Union Biometrica