Applications & Publications
Technical Notes
Development of a High-Throughput Approach for Imaging and Sorting of Organoids and Spheroids using the COPAS VISION (QTN-029)
May 08, 2020
Tumor spheroids -- Analysis, sorting and imaging of cell aggregates from various cell types for HTS purposes using COPAS VISION 500 (QTN-027)
April 21, 2020
Publications
Image-assisted Large Particle Flow Cytometry and Plate-Based Sorting of Intact 3D Models to Support Reproducible New Approach Methodologies
ESTIV 2026
Harrison et al.
June 29, 2026
Image-assisted Large Particle Flow Cytometry and Plate-Based Sorting of Intact 3D Models to Support Reproducible New Approach Methodologies
Large Particle Flow Cytometry Platform for High-Throughput Analysis, Brightfield Imaging, and Precision Dispensing of Intact HT-29 and HT-1080 Spheroids
CYTO 2026
Harrison et al.
June 06, 2026
Large Particle Flow Cytometry Platform for High-Throughput Analysis, Brightfield Imaging, and Precision Dispensing of Intact HT-29 and HT-1080 Spheroids
Analysis, imaging and sorting of 3-D tumor spheroids on the COPAS Vision Large Particle Flow Cytometer
EACR 2026
Ferrero et al.
May 21, 2026
Analysis, imaging and sorting of 3-D tumor spheroids on the COPAS Vision Large Particle Flow Cytometer
Triple co-culture spheroid models of lung and ovarian carcinoma cell lines for the in vitro testing of antitumor liposomes
Shah et al. June 05, 2025 Received 19 Feb 2025, Accepted 26 May 2025, Published online: 05 Jun 2025; Journal of Liposome Research, DOI: 10.1080/08982104.2025.2514850
View AbstractTriple co-culture spheroid models of lung and ovarian carcinoma cell lines for the in vitro testing of antitumor liposomes
Tumor cells cultured as spheroids have been shown to be superior to tumor cells cultured in monolayers as in vitro models of solid tumors because they exhibit features of the tumor microenvironment (TME) such as cell-cell interactions, extracellular matrix and diffusional gradients. However, spheroids composed solely of tumor cells, i.e. monoculture spheroids, still lack the non-tumor cell components that contribute to additional in vivo TME complexity. This study, explored the development of triple co-culture spheroid models incorporating tumor cells, tissue specific fibroblasts, and endothelial cells to mimic more of the features of the in vivo TME. Using a modified liquid overlay technique, triple co-culture spheroids were successfully generated for both drug resistant lung tumor cells as well as drug resistant ovarian tumor cells. The triple co-culture models exhibited several characteristics of in vivo tumors, including extracellular matrix (ECM) production and distinct spatial locations of cell types. Notably, fibroblasts remained in the core as the spheroid grew, while endothelial cells were found in the core only in the presence of fibroblasts. A liposomal formulation previously shown in monolayer cultures to have selective toxicity toward multiple drug resistant tumor cell types was significantly less toxic and showed composition-dependent levels of toxicity in spheroid cultures with multiple cell types. These findings demonstrate that triple co-culture spheroids can serve as in vitro models that more closely mimic in vivo tumor characteristics to facilitate the optimization of antitumor therapies prior to in vivo testing.
Easy mass production of homogenous and uniform 3D spheroids for high-throughput screening applications
Leu et al. July 03, 2020 abc bioapply: https://biopply.com/applications-and-3d-laboratory-services/hts-hcs-applications/