Applications & Publications
Technical Notes
Automated Analysis and Sorting of 1st instar Larvae of the Malaria Mosquito Anopheles sp. (QTN-012)
Publications
Engineering Resilient Gene Drives Towards Sustainable Malaria Control: Predicting, Testing and Overcoming Target Site Resistance
Morianou et al. October 21, 2024 bioRxiv preprint doi: https://doi.org/10.1101/2024.10.21.618489
Engineering Resilient Gene Drives Towards Sustainable Malaria Control: Predicting, Testing and Overcoming Target Site Resistance
Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages
D'Amato et al. February 21, 2024 Nat Commun 15, 952 (2024). https://doi.org/10.1038/s41467-024-44907-x
Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages
Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation
Fuchs et al. October 05, 2021 PLoS Genet 17(10): e1009740. https://doi.org/10.1371/journal. pgen.1009740
Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation
Gene-drive suppression of mosquito populations in large cages as a bridge between lab and field
Hammond A. et al. July 28, 2021 Nature Commun.;12(1):4589 • PMID: 34321476 • DOI: 10.1038/s41467-021-24790-6
Gene-drive suppression of mosquito populations in large cages as a bridge between lab and field
Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance
Hammond et al. January 29, 2021 PLoS Genet 17(1): e1009321. https://doi.org/10.1371/journal. pgen.1009321
Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance
Efficient Production of Male Wolbachia-infected Aedes Aegypti Mosquitoes Enables Large-Scale Suppression of Wild Populations
Crawford et al. April 06, 2020 Nature Biotechnology volume 38, pages 482–492(2020) • PMID: 32265562 • DOI: 10.1038/s41587-020-0471-x
Efficient Production of Male Wolbachia-infected Aedes Aegypti Mosquitoes Enables Large-Scale Suppression of Wild Populations
A New Role of the Mosquito Complement-like Cascade in Male Fertility in Anopheles gambiae
Julien Pompon1 ,¤ and Elena A. Levashina1 ,2 September 22, 2015 PLoS Biol. 2015 Sep; 13(9): e1002255. Published online 2015 Sep 22. doi: 10.1371/journal.pbio.1002255
View AbstractA New Role of the Mosquito Complement-like Cascade in Male Fertility in Anopheles gambiae
Tools for Anopheles gambiae Transgenesis.
Volohonsky G1, Terenzi O11 Soichot J1, Naujoks DA2, Nolan T2, Windbichler N2, Kapps D1, Smidler AL1, Vittu A1, Costa G3, Steinert S1, Levashina EA3, Blandin SA1, Marois E 4 April 13, 2015 G3 (Bethesda). 2015 Apr 13;5(6):1151-63. doi: 10.1534/g3.115.016808.
View AbstractTools for Anopheles gambiae Transgenesis.
1) INSERM U963, CNRS UPR9022, Université de Strasbourg, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg, France.
2) Imperial College London, Division of Cell and Molecular Biology, Imperial College Road, London SW7 2AZ, United Kingdom.
3) Department of Vector Biology, Max-Planck Institute for Infection Biology, Charitéplatz 1, 10117 Berlin, Germany.
4) INSERM U963, CNRS UPR9022, Université de Strasbourg, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg, France
A synthetic sex ratio distortion system for the control of the human malaria mosquito
Roberto Galizi¹,², Lindsey A. Doyle³, Miriam Menichelli¹, Federica Bernardini¹, Anne Deredec¹, Austin Burt¹, Barry L. Stoddard³, Nikolai Windbichler¹,* & Andrea Crisanti¹,²,* June 10, 2014 NATURE COMMUNICATIONS | 5:3977 | DOI: 10.1038/ncomms4977 |www.nature.com/naturecommunications
View AbstractA synthetic sex ratio distortion system for the control of the human malaria mosquito
1) Department of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK. 2) Centro di Genomica Funzionale, University of Perugia, Dipartimento di Medicina Sperimentale Via Gambuli, Edificio D, 3 Piano, 06132 Perugia, Italy. 3) Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. * These authors contributed equally to this work.
Targeted Mutagenesis in the Malaria Mosquito Using TALE Nucleases
Andrea L. Smidler, Olivier Terenzi, Julien Soichot, Elena A. Levashina, Eric Marois August 15, 2013 Published: August 15, 2013 • DOI: 10.1371/journal.pone.0074511
Targeted Mutagenesis in the Malaria Mosquito Using TALE Nucleases
High-throughput sorting of mosquito larvae for laboratory studies and for future vector control interventions
Eric Marois¹,Christina Scali²,Julien Soichot¹,Christine Kappler¹, Elena A Levashina¹,³,*,†,Flaminia Catteruccia4,5,**,† August 28, 2012 Malaria Journal 2012, 11:302 doi:10.1186/1475-2875-11-302 1) Institut de Biologie Moléculaire et Cellulaire, INSERM U963, CNRS UPR9022,15 rue René Descartes, 67084 Strasbourg, France 2) Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, London SW7 2AZ, UK 3) Department of Vector Biology, Max-Planck Institute for Infection Biology,Chariteplatz 1, 10117 Berlin, Germany 4) Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università degli Studi di Perugia, Terni 05100, Italy 5) Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA * Corresponding author. Department of Vector Biology, Max-Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany ** Corresponding author. Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA † Equal contributors.
View AbstractHigh-throughput sorting of mosquito larvae for laboratory studies and for future vector control interventions
Background: Mosquito transgenesis offers new promises for the genetic control of vector-borne infectious diseases such as malaria and dengue fever. Genetic control strategies require the release of large number of male mosquitoes into field populations, whether they are based on the use of sterile males (sterile insect technique, SIT) or on introducing genetic traits conferring refractoriness to disease transmission (population replacement). However, the current absence of high-throughput techniques for sorting different mosquito populations impairs the application of these control measures.
Methods: A method was developed to generate large mosquito populations of the desired sex and genotype. This method combines flow cytometry and the use of Anopheles gambiae transgenic lines that differentially express fluorescent markers in males and females.
Results: Fluorescence-assisted sorting allowed single-step isolation of homozygous transgenic mosquitoes from a mixed population. This method was also used to select wild-type males only with high efficiency and accuracy, a highly desirable tool for genetic control strategies where the release of transgenic individuals may be problematic. Importantly, sorted males showed normal mating ability compared to their unsorted brothers.
Conclusions: The developed method will greatly facilitate both laboratory studies of mosquito vectorial capacity requiring high-throughput approaches and future field interventions in the fight against infectious disease vectors.
An Anopheles transgenic sexing strain for vector control
Flaminia Catteruccia1, 2, Jason P Benton1, 2 & Andrea Crisanti1 October 09, 2005 Nature Biotechnology 1) Imperial College London, Department of Biological Sciences, Imperial College Road, London SW7 2AZ, UK. 2) These authors contributed equally to this work.
View AbstractAn Anopheles transgenic sexing strain for vector control
Genetic manipulation of mosquito species that serve as vectors for human malaria is a prerequisite to the implementation of gene transfer technologies for the control of vector-borne diseases. Here we report on the development of transgenic sexing lines for the mosquito Anopheles stephensi, the principal vector of human malaria in Asia. Male mosquitoes, expressing enhanced green fluorescent protein (EGFP) under the control of the β2-tubulin promoter, are identified by their fluorescent gonads in as early as their 3rd instar larval stage, and can be efficiently separated from females using both manual methods and automated sorting machines. Importantly, β2-EGFP males are not impaired in their mating ability and viable fluorescent spermatozoa are also detected in spermathecae of wild-type females mated with transgenic males. The transgenic mosquito lines described here combine most of the features desired and required for a safe application of transgenic methodologies to malaria-control programs.