Applications & Publications
Publications
Mass Purification Protocol for Drosophila melanogaster Wing Imaginal Discs: An Alternative to Dissection to Obtain Large Numbers of Disc Cells
Hoareau et al. September 22, 2022 Biology (Basel) 2022 Oct; 11(10): 1384. Published online 2022 Sep 22. doi:Â 10.3390/biology11101384
View AbstractMass Purification Protocol for Drosophila melanogaster Wing Imaginal Discs: An Alternative to Dissection to Obtain Large Numbers of Disc Cells
Drosophila melanogaster imaginal discs are larval internal structures that become the external organs of the adult. They have been used to study numerous developmental processes for more than fifty years. Dissecting these imaginal discs for collection is challenging, as the size of third-instar larvae organs is typically less than 1 mm. Certain experimental applications of the organs require many cells, which requires researchers to spend several hours dissecting them. This paper proposes an alternative to dissection in the form of a mass enrichment protocol. The protocol enables the recovery of many wing imaginal discs by grinding large quantities of third-instar larvae and separating the organs using filtration and a density gradient. The wing imaginal discs collected with this protocol in less than three hours are as well preserved as those collected by dissection. The dissociation and filtration of the extract allow the isolation of a large amount of wing imaginal disc cells.
A large-scale, in vivo transcription factor screen defines bivalent chromatin as a key property of regulatory factors mediating Drosophila wing development.
Schertel C¹, Albarca M, Rockel-Bauer C¹, Kelley NW, Bischof J¹, Hens K, van Nimwegen E, Basler K¹, Deplancke B¹. April 25, 2015 Genome Res. 2015 Apr;25(4):514-23. doi: 10.1101/gr.181305.114. Epub 2015 Jan 7.
View AbstractA large-scale, in vivo transcription factor screen defines bivalent chromatin as a key property of regulatory factors mediating Drosophila wing development.
1Institute of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland
Drosophila Fluorescent-Imaginal Disc Analysis and Sorting by COPAS Flow Cytometry Technique (412A)
45th Annual Drosophila Research Conference, March 24-28, 2004
Daniela Panáková1, Lydia Michaut2, Rico Bongaarts3, Bo Wang4, Julia Thompson4, Rock Pulak4
March 24, 2004
Drosophila Fluorescent-Imaginal Disc Analysis and Sorting by COPAS Flow Cytometry Technique (412A)
1) Max Planck Institute, CBG, Susan Eaton Lab (Dresden, Germany), 2) Biozentrum, Walter Gehring lab (Basel, Switzerland), 3) Union Biometrica (Geel, Belgium), 4) Union Biometrica Inc. (Holliston, MA, USA).