Applications & Publications
Technical Notes
Large Particle Flow Cytometry Allows High Quality Isolation of Viable Cardiomyocytes and Other Myocytes (QTN-028)
April 22, 2020
Automated Analysis and Sorting of Cardiomyocyte Stem Cell Clusters (QTN-001)
Use of the COPAS Select for the analysis and sorting of individual embryoid bodies (EBs) from cultured samples. Stem cell aggregates were accurately dispensed into multi-well plates and visually inspected for viability. (QTN-001)
An Overview of COPAS™ Large Particle Flow Cytometry for the Analysis and Sorting of Large Cells and Cell Clusters including Stem Cells, Embryoid Bodies and Cardiomyocytes (QTN-013)
Publications
In Vivo Bioincubation Promotes Maturation of Human iPSC-Derived Cardiomyocytes in Neonatal Rat and Pig Hearts
Wang et al. July 22, 2026 bioRxiv [Preprint]. 2026 Jul 22:2026.07.21.739858. [Version 1] doi: 10.64898/2026.07.21.739858
View AbstractIn Vivo Bioincubation Promotes Maturation of Human iPSC-Derived Cardiomyocytes in Neonatal Rat and Pig Hearts
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great promise for cardiac regenerative medicine and disease modeling. However, hiPSC-CMs generated through conventional in vitro differentiation exhibit immature, fetal-like phenotypes. While in vivo bioincubation in neonatal rodent hearts promotes hiPSC-CM maturation toward adult-like phenotypes, studies in large animal models remain limited, particularly with detailed morphological characterization. In this study, we investigated bioincubation of fluorescently labeled hiPSC-CMs in both neonatal rat and pig hearts. Human iPSCs were differentiated into cardiomyocytes expressing GFP or RFP reporters and subsequently injected intramyocardially into neonatal rats (GFP-labeled) and pigs (RFP-labeled). After 4–8 weeks of bioincubation, fluorescent hiPSC-CMs were isolated using large-particle fluorescence-activated cell sorting (COPAS), which preserves cellular morphology of adult-like cardiomyocytes. Immunostaining for cardiac troponin T revealed well-organized sarcomeric structures in multinucleated hiPSC-CMs. Bioincubated hiPSC-CMs displayed rod-shaped morphology with binucleation, characteristic features of mature adult cardiomyocytes. Quantitative analysis demonstrated that bioincubated hiPSC-CMs from rat hearts exhibited sarcomere length and cell circularity comparable to native rat adult cardiomyocytes, though with higher intra-cellular variability in sarcomere organization. Histological examination confirmed successful engraftment of RFP-positive hiPSC-CMs within pig myocardium, with engrafted cells also displaying mature adult-like features. These findings provide critical proof-of-concept data for bioincubation in large animal models and support further investigation for disease modeling, drug screening, and regenerative cell therapies.
ZebraReg-a novel platform for discovering regulators of cardiac regeneration using zebrafish
Apolínová et al. May 10, 2024 Front Cell Dev Biol. 2024; 12: 1384423. Published online 2024 May 10. doi: 10.3389/fcell.2024.1384423
View AbstractZebraReg-a novel platform for discovering regulators of cardiac regeneration using zebrafish
Cardiovascular disease is the leading cause of death worldwide with myocardial infarction being the most prevalent. Currently, no cure is available to either prevent or revert the massive death of cardiomyocytes that occurs after a myocardial infarction. Adult mammalian hearts display a limited regeneration capacity, but it is insufficient to allow complete myocardial recovery. In contrast, the injured zebrafish heart muscle regenerates efficiently through robust proliferation of pre-existing myocardial cells. Thus, zebrafish allows its exploitation for studying the genetic programs behind cardiac regeneration, which may be present, albeit dormant, in the adult human heart. To this end, we have established ZebraReg, a novel and versatile automated platform for studying heart regeneration kinetics after the specific ablation of cardiomyocytes in zebrafish larvae. In combination with automated heart imaging, the platform can be integrated with genetic or pharmacological approaches and used for medium-throughput screening of presumed modulators of heart regeneration. We demonstrate the versatility of the platform by identifying both anti- and pro-regenerative effects of genes and drugs. In conclusion, we present a tool which may be utilised to streamline the process of target validation of novel gene regulators of regeneration, and the discovery of new drug therapies to regenerate the heart after myocardial infarction.
Trajectory reconstruction identifies dysregulation of perinatal maturation programs in pluripotent stem cell-derived cardiomyocytes
Kannan et al. April 25, 2023 PMID: 37014753 PMCID: PMC10545814 DOI: 10.1016/j.celrep.2023.112330
Trajectory reconstruction identifies dysregulation of perinatal maturation programs in pluripotent stem cell-derived cardiomyocytes
Transcriptomic entropy quantifies cardiomyocyte maturation at single cell level
Kannan et al. April 03, 2020 PMID: 34534204 PMCID: PMC8448341 DOI: 10.1371/journal.pcbi.1009305
Transcriptomic entropy quantifies cardiomyocyte maturation at single cell level
PGC1/PPAR Drive Cardiomyocyte Maturation through Regulation of Yap1 and SF3B2
Murphy et al. February 07, 2020 bioRxiv preprint: doi: https://doi.org/10.1101/2020.02.06.937797
PGC1/PPAR Drive Cardiomyocyte Maturation through Regulation of Yap1 and SF3B2
Comparison of Zebrafish Larvae and hiPSC Cardiomyocytes for Predicting Drug-Induced Cardiotoxicity in Humans
Sylvia Dyballa et al. October 01, 2019 Toxicological Sciences, Volume 171, Issue 2, October 2019, Pages 283–295, https://doi.org/10.1093/toxsci/kfz165
Comparison of Zebrafish Larvae and hiPSC Cardiomyocytes for Predicting Drug-Induced Cardiotoxicity in Humans
Large Particle Fluorescence-Activated Cell Sorting Enables High-Quality Single-Cell RNA Sequencing and Functional Analysis of Adult Cardiomyocytes
Kannan et al. August 15, 2019 PMID: 31415233 PMCID: PMC6699769 DOI: 10.1161/CIRCRESAHA.119.315493