Publications

Flow Cytometry Publications - Bead Based Assays

Directed Differentiation of Embryonic Stem Cells Using a Bead-Based Combinatorial Screening Method

Marina Tarunina, Diana Hernandez, Christopher J. Johnson, Stanislav Rybtsov, Vidya Ramathas, Mylvaganam Jeyakumar, Thomas Watson, Lilian Hook, Alexander Medvinsky, Chris Mason, Yen Choo
September 24, 2014
http://dx.doi.org/10.1371/journal.pone.0104301

High throughput screening of one-bead-one-compound peptide libraries using intact cells

Choi-Fong Cho, Babak Behnam Azad, Leonard G. Luyt, and John Lewis
July 02, 2013
ACS Comb. Sci., Just Accepted Manuscript • DOI: 10.1021/co4000584 • Publication Date (Web): 02 Jul 2013

Flow-based pipeline for systematic modulation and analysis of 3D tumor microenvironments.

Li CY, Wood DK, Huang JH, Bhatia SN.
April 23, 2013
Lab Chip. 2013 Apr 23;13(10):1969-78. doi: 10.1039/c3lc41300d. Epub 2013 Apr 5.

Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

A two-channel detection method for autofluorescence correction and efficient on-bead screening of one-bead one-compound combinatorial libraries using the COPAS fluorescence activated bead sorting system

Martin Hintersteiner¹ and Manfred Auer²
March 12, 2013
2013 Methods Appl. Fluoresc. 1 017001 doi:10.1088/2050-6120/1/1/017001

Process Automation toward Ultra-High-Throughput Screening of Combinatorial One-Bead-One-Compound (OBOC) Peptide Libraries

Junhoe Cha, Jaehong Lim, Yiran Zheng, Sylvia Tan, Yi Li Ang, Jessica Oon, Mei Wei Ang, Jingjing Ling, Marcus Bode, Su Seong Lee
June 17, 2012
Journal of Laboratory Automation 2012 17: 186 originally published online 14 February 2012

Institute of Bioengineering and Nanotechnology, Singapore.

TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress

Fernando Gómez-Herreros, Lola de Miguel-Jiménez, Macarena Morillo-Huesca, Lidia Delgado-Ramos, María C. Muñoz-Centeno and Sebastián Chávez
April 27, 2012
Nucleic Acids Research, 2012, 1–12

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avda Reina Mercedes 6. E-41012 Seville, Spain

Microbial encapsulation in monodisperse hydrogel microspheres enables fast and sensitive phenotypic analyses using flow cytometers

Applications of Flow Cytometry in Microbiology Theme Day
Lidia Delgado¹, Gloria Jurado², Gema Galayo², Elena Ogalla², Lourdes Moreno³, Juan C Rodríguez-Aguilera4, Ángel Cebolla5, Carolina Sousa³, María Flores² and Sebastián Chávez¹
February 28, 2011

1) Department of Genetics, Universidad de Sevilla, Seville, Spain 2) Ingeniatrics Tecnologías SL, Spain 3) Department of Microbiology and Parasitology, Universidad de Sevilla, Seville, Spain 4) Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Seville, Spain 5) Biomedal SL, Spain

 

Multiplexed, high-throughput analysis of 3D microtissue suspensions.

Chen AA, Underhill GH, Bhatia SN
October 04, 2010
Integr Biol (Camb). 2010 Oct 4;2(10):517-27. Epub 2010 Sep 1.

Massachusetts Institute of Technology, Cambridge, 02139, USA.

3-nitro-tyrosine as an internal quencher of autofluorescence enhances the compatibility of fluorescence based screening of OBOC combinatorial libraries.

Jared Townsend, Andrew Do, Alan Lehman, Seth Dixon, Babak Sanii and Kit S. Lam*
June 01, 2010
Comb Chem High Throughput Screen. 2010 Jun;13(5):422-9.

 Division of Hematology & Oncology, Department of Internal Medicine, UC Davis Cancer Center, University of California Davis, California, USA

Accurate MALDI-TOF/TOF sequencing of one-bead-one-compound peptide libraries with application to the identification of multiligand protein affinity agents using in situ click chemistry screening.

Lee SS, Lim J, Tan S, Cha J, Yeo SY, Agnew HD, Heath JR.
January 15, 2010
Anal Chem 2010 Jan 15;82(2):672-9

Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669

Spectral Measurements of Large Particles by Flow Cytometry

Dakota A. Watson,¹ Daniel F. Gaskill,¹ Leif O. Brown,² Stephen K. Doorn,²and John P. Nolan¹*
May 01, 2009
Cytometry A. 2009 May; 75(5): 460–464. doi: 10.1002/cyto.a.20706.

1La Jolla Bioengineering Institute, La Jolla CA 92037
2Los Alamos National Laboratory, Los Alamos NM 97545

Novel method for high-throughput colony PCR screening in nanoliter-reactors

Marcel Walser¹, Rene Pellaux¹, Andreas Meyer¹, Matthias Bechtold¹, Herve Vanderschuren², Richard Reinhardt³, Joseph Magyar4, Sven Panke¹ and Martin Held¹,*
February 26, 2009
Nucl. Acids Res. (2009) 37 (8): e57/1-e57/8

1) ETH Zurich, Institute of Process Engineering, BioProcess Laboratory (BPL), 2) ETH Zurich, Institute of Plant Science, Plant Biotechnology, Zurich, Switzerland, 3) Max Planck Institute for Molecular Genetics, Analytics & Computing, Berlin, Germany and 4) Harlan Laboratories Ltd., Environmental Safety and Metabolism, Itingen, Switzerland

Isolation of monoclonal microcarriers colonized by fluorescent E. coli

Walser M, Leibundgut RM, Pellaux R, Panke S, Held M.
June 16, 2008
ETH Zurich, Institute of Process Engineering, BioProcess Laboratory, Universitätsstr. 6/CAB H88, CH-8092 Zurich, Switzerland.

Microencapsulation can be used for high-throughput (HT) processing of bacterial libraries. Ideally, each microcarrier would contain exactly one cell or colony. However, synthesis yields a mixture of capsules containing the desired one cell,  multiple cells and empty microcarriers. E. coli cells expressing green fluorescent protein (GFP) were encapsulated in 35 nl Hydrogel carriers. HT sorting procedures by a COAPS sorter were then used to enrich samples to 95% monoclonality.

Published Online: Jun 16 2008 2:41PM; DOI: 10.1002/cyto.a.20597; Cytometry A. 2008 Sep;73(9):788-98.

Aminodeoxychorismate Synthase Inhibitors from One-Bead One-Compound Combinatorial Libraries: “Staged” Inhibitor Design

Seth Dixon, Kristin T. Ziebart, Ze He, Mellisa Jeddeloh, Choong Leol Yoo, Xiaobing Wang,§ Alan Lehman,§ Kit S. Lam, § Michael D. Toney,*,† and Mark J. Kurth*,†
November 10, 2006
J. Med. Chem., 49 (25), 7413 -7426, 2006. 10.1021/jm0609869 S0022-2623(06)00986-1

Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, Department of Chemistry, Central Connecticut State University, 1615 Stanley Street, New Britain, Connecticut 06050, and §Department of Internal Medicine, Division of Hematology and Oncology, UC Davis Cancer Center, 4501 X Street, Sacramento, California 95817

SIRT1 Top 40 Hits: Use of One-Bead, One-Compound Acetyl-Peptide Libraries and Quantum Dots to Probe Deacetylase Specificity.

Adam L. Garske and John M. Denu
December 13, 2005
Biochemistry 45(1) pp 94 – 101 (2006)

Departments of Biomolecular Chemistry and Chemistry, University of Wisconsin, Madison, Wisconsin 53706

OBOC Small-Molecule Combinatorial Library Encoded by Halogenated Mass-Tags

Sung Hee Hwang, Alan Lehman, Xin Cong, Marilyn M. Olmstead, Kit S. Lam, Carlito B. Lebrilla, and Mark J. Kurth
September 17, 2004
Organic Letters, Volume 6(21), Pages 3829-3832

Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, California 95616-5295, and Division of Hematology and Oncology, Department of Internal Medicine, University of California-Davis Cancer Center, 4501 X Street, Sacramento, California 95817

Combinatorial library of peptidotriazoles: identification of [1,2,3]-triazole inhibitors against a recombinant Leishmania mexicana cysteine protease.

Tornoe CW, Sanderson SJ, Mottram JC, Coombs GH, Meldal M.
May 01, 2004
J Comb Chem. 2004 May-Jun;6(3):312-24.

Center for Solid-Phase Organic Combinatorial Chemistry, Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.

Solid Phase Combinatorial Library of 1,3-Azole Containing Peptides for the Discovery of Matrix Metallo Proteinase Inhibitors

Caspar Christensen1, Christine Bruun Schiødt 2, Niels Tækker Foged2, Morten Meldal1 *
December 08, 2003
QSAR & Combinatorial Science, Volume 22, Issue 7, Pages 754-766

1) Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark, 2) Nordic Bioscience A/S, Herlev Hovedgade 207, DK-2730 Herlev, Denmark.

New Technology Automates Sorting of Large, Bead-based CombiChem Libraries, P127.

18th American Peptide Symposium, July 19-23, 2003
K. Ver Donck1, L. Bols1, R. Bongaarts1, P. Van Osta1, A. J. Brouwer2, R. M. J. Liskamp2, D. Perrault3, K. Kalutkiewicz3, J. Geysen1, and R. Pulak3
July 19, 2003

Union Biometrica-(Geel, BE1, Holliston, MA USA3), Utrecht University2 (Utrecht, NL).

From synthetic receptors towards synthetic antibodies using combinatorial chemistry, IN4.

2nd European Symposium on Combinatorial Sciences (Eurocombi 2), June 29-July 3
Rob M.J. Liskamp, Arwin J. Brouwer, Christina Chamorro, Menno C.F. Monnee, Till Opatz, Roland J. Pieters, Dirk T.S. Rijkers, Kirk-Jan van Zoelen.
June 29, 2003

Department of Medicinal Chemistry, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, (Utrecht, The Netherlands).

Protein affinity profiling using solid-phase combinatorial libraries, O19.

2nd European Symposium on Combinatorial Sciences (Eurocombi 2), June 29-July 3
Anita J. Mathiesen, Sheryl Surve, Haifeng Yin, Phaedria M. St.Hilaire.
June 29, 2003

Carlsberg Biosectro, Valby (Denmark).

Bead-Based assays using COPAS™ Flow Sorting, P16.

2nd European Symposium on Combinatorial Sciences (Eurocombi 2), June 29-July 3
K. Ver Donck1, L. Bols1, R. Bongaarts1, P. Van Osta1, A. J. Brouwer2, R. M. J. Liskamp2, D. Perrault3, J. Geysen1, and R. Pulak3
June 29, 2003

Union Biometrica-(Geel, BE1, Holliston, MA USA3), Utrecht University2 (Utrecht, NL).

Bead Based Assays Using COPAS Flow Sorting, Practical Session

Innovative Combinatorial Approaches and Technologies, Practical Training Course (Florence, Italy) April 9-11, 2003
R. Bongaarts and A. Comas.
April 09, 2003

Union Biometrica, Geel, Belgium.

New technology automates sorting of large, bead--based combinatorial chemistry libraries, #87.

Lab Automation 2003, February 1-5, 2003, Palm Springs, CA
K. VerDonck1, L. Bols1, R. Bongaarts1, P. Van Osta1, J. Brouwer2, R. M. J. Liskamp2, J. Geysen1, D. Perrault3, K. Kalutkiewicz3, R. Pulak3
February 01, 2003

Union Biometrica 1, 3, (Geel, BE 1; Holliston, MA 3), Utrecht University (Utrecht, NL 2)

Assays for Bead-Based Technologies Using COPAS™ Flow Sorting, Session 3, Abstract 11.

ISLAR 2002, October 20-23, 2002
K. Ver Donck1, L. Bols1, R. Bongaarts1, P. Van Osta1, A. J. Brouwer2, R. M. J. Liskamp2, J. Geysen1, D. Perrault3, K. Kalutkiewicz3, R. Pulak3
October 20, 2002

Union Biometrica-ESO 1, (Geel, BE), Union Biometrica 3, (Holliston, MA) Utrecht University 2 (Utrecht, NL)

The one-bead two-compound assay for solid-phase screening of Combinatorial libraries.

Morton Meldal
September 16, 2002
Biopolymers, 66(2) 93-100.

Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.

At the higher end of High Content Screening: The MIAS HTS imaging device, #82.

MipTec-ICAR, May 27-30, 2002
Kris Ver Donck, Luc Bols, Peter Van Osta, Johan Geysen
May 27, 2002

Union Biometrica, Cipalstraat 3, B-2440 Geel, Belgium

Firms Creating More Focused Compound Libraries, Saving Time and Money in Drug Discovery Research

Nina Flanagan
November 15, 2001
Genetic Engineering News, Vol. 21, No. 20, November 15, 2001

Novel Methods for Analysis of Solid Phase Combinatorial Libraries on PEG-based Resins, #1134.

84th CSC Conference and Exhibition, May 2001
M. Meldal*, C. Gotfredsen, A. Papanikos, A. Meissner, M. Grotli, T. Groth, J. Beyer, J. Duus, C. Tornoe, and P. St. Hilaire
May 01, 2001

SPOCC-center, Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.

 

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