COPAS Accessory Modules
A variety of optional modules are available to add specific enhanced capabilities to your COPAS FP system including choices of lasers, larger sample cups and an auto-sampler.
COPAS FP can accommodate up to three excitation lasers. The 488nm is standard with many customers also adding a 405nm, 561nm or 640nm. Please inquire if your research requires a different excitation wavelength. Customers who may want to add a laser at a later time should be aware that the optics for these lasers can not be retro-fitted in the field. A factory alignment procedure is required unless “Laser Ready Optics” have been ordered with the initial purchase. The two best choices are to either order (A) the complete laser + optics with the initial system, or to at least add the (B) “Laser Ready Optics” as part of the initial factory build.
Bulk samples are introduced via a standard 50 ml conical tube (40 ml working volume) with suspended stirrer. For larger samples optional 750 ml and 1500 ml stirred sample cups are available.
Rotating Sample Chamber and Non-metallic FOCA
The Rotating Sample Chamber is designed specifically for handling extremely delicate samples (adipocytes, plant protoplasts, etc.) and for samples that tend to either float or sink in a sample cup with normal stirring-type agitation. Chamber can be used with 250 & 500 micron FOCAs (order separately).
The 500 Micron Metallic-free FOCA is designed for handling adipocytes and other delicate samples where contact with metal surfaces must be avoided. This FOCA can accommodate objects 20 – 400 microns in diameter and is optimized for objects 40 – 300 microns in diameter. Use with the Rotating Sample Introduction Chamber with Integrated Agitator (order separately) for a completely metal-free sample path. Typical use with a mouse adipocyte preparation enables analyzing 100 objects/second and dispensing intact adipocytes constituting 10% of the preparation.
Rotating sample cup for fragile samples and samples that float or sink
LP Sampler™ (Large Particle Sampler)
This is the only automated sample introduction system designed specifically for gentle handling of large, fragile objects such as
- delicate cells/clusters (stem cell clusters, neurospheres, tumor spheroids)
- viable small multicellular model organisms (nematodes, Drosophila, zebrafish larvae)
- beads used as micro-carriers for clonal libraries
- seeds and fragile plant tissue
This autosampler is ideal for objects ranging from 10 to 1500 microns in diameter. It is capable of gently aspirating those samples from multiwell plates or a petri dish and delivering them intact to Union Biometrica’s COPAS FP™, BioSorter® or VAST BioImager™ systems. It has the flexibility to handle a variety of standard or deep well plates including 24, 96 and 384.