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PROTOCOLS

Sample Preparation

Instruments

Sample Preparation

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Sample Preparation Protocols
These protocols are recommendations only. Since each experiment design varies, other protocols may be preferable. If you have questions, please contact Union Biometrica.

C. elegans Sample Prep
C. elegans Washing Technique
C. elegans Sucrose Float Technique
C. elegans Sucrose Sedimentation Technique
C. elegans Obtaining synchronous cultures of hatchlings
C. elegans M9 Buffer Formulation
Drosophila Embryos
Drosophila Formulation of Fly Wash
Drosophila Larvae Preparation
Drosophila Fly Food Prep
Drosophila Viability Evaluation
Drosophila L Growth Media Prep

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Instruments

You will need Adobe Acrobat Reader to view or download these files.

Instrument Protocols
These protocols are recommendations only. Since each experiment design varies, other protocols may be preferable. If you have questions, please contact Union Biometrica.

GENERAL INSTRUMENT

Stylus Wire Cleaning Protocol
Custom 96-well Plates for Fly Growth Analysis
Sflex Custom Well Filling Templates

BIOSORT

Hypochloride Cleaning Protocol
Sterilization Protocol
Fluorescent Bead Protocol

Sort Optimization Protocol
C. elegans Batch Sorting of a Mixed Population
C. elegans Gain Settings by Stage
Lo-Level Fluorescent Bead Controls

SELECT

Hypochloride Cleaning Protocol
Sterilization Protocol

EXPRESS -same as SELECT

PLUS

Hypochloride Cleaning
Sterilization
Sort Optimization Protocol Using UV Laser
Sort Optimization Protocol for Beads

Hypochloride Cleaning
Sterilization
Sort Optimization Protocol

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