Sorted Events Newsletter, Issue 505 Automated Identification and Sorting of Sex-specific GFP-expressing Drosophila Embryos
An excerpt from a poster presented at the 45th Annual Drosophila Research Conference.
	The COPAS SELECT and EXPRESS instruments are able to analyze and sort Drosophila embryos on the basis of size and fluorescence signals. One application is to sort embryos that have sex-specific GFP-expression, for example males and females can be separated from each other at the embryonic stage and thereby simplify the isolation of virgin females for subsequent genetic crosses. This also allows for the collection of embryonic samples for biochemical studies of early sex-specific differences, at a stage before morphological differences between males and females become evident. For our experiment, two different transgenic Drosophila strains that carried EGFP under the control of Sxl PE promoter were tested on the COPAS instrument. One strain has the transgene integrated on the X-chromosome and one has the integration on Chromosome 3. We show that using either of the strains, it is possible to sort embryos on the basis of green fluorescence and separate males from females, with better than 99% accuracy. CLICK HERE to View the Poster in .PDF or visit: http://www.unionbio.com for more information.
About the instrument: Based on similar principles as flow cytometry, the COPAS™ instrument from Union Biometrica provides an automated alternative to manual sorting for increased speed, sensitivity, quantification, and repeatability of experiments. The COPAS SELECT instrument analyzes and sorts small model organisms and other objects (from 120-300 microns) on the basis of size, density, and fluorescence signals. A gentle pneumatic sorting mechanism is used to permit the analysis and handling of sensitive organisms and tissues with a high level of recovery and a low level of lethality.
Union Biometrica, Inc. uses "Sorted Events" email newsletters to share information about new, innovative techniques for automated sorting techniques designed for a wide range of applications including: cells, small model organisms, seeds, beads, and other particles (40-1500 microns). For more information, please call (617) 591-1211, e-mail: sales@unionbio.com or visit us on the web at http://www.unionbio.com Union Biometrica, Inc. • 35 Medford Street • Suite 101 • Somerville, MA 02143 • USA
Unsubscribe Information: This email was sent to: %0%, by Union Biometrica. If you are not interested in receiving Sorted Events Newsletters from Union Biometrica, Inc., please send an email to remove@unionbio.com.

.