Sorted Events Newsletter, Issue 506 Automated Analysis & Sorting of C. elegans
Analysis & Sorting of Acridine Orange Stained C.elegans with Increased Germline Apoptosis
ced-9(n 1653) adult mutants observed by differential interference confocal microscopy (above) and same animal stained with AO (below)	Acridine orange, a commonly used dye in flow cytometry, differentially stains double-stranded DNA to a green fluorescence and single stranded DNA and RNA to an orange fluorescence. This metachromatic fluorescence is sensitive to DNA Conformation, making it a useful probe for detective apoptotic cells. Recent experiments show that acridine orange stained apoptotic corpses in ced-9(n1653) mutant C.elegans gonad can be detected and analyzed, and positive worms could be sorted on the COPAS BIOSORT flow cytometry instrument, with a high level of accuracy. That screening can take place in living worms has several advantages over other staining methods such as: stained worms can be recovered and propagated, making this stain ideal for genetic screening; and normal morphology is maintained in the worms as no permeablization or fixation is required. CLICK HERE to see a full description of .PDF or visit: http://www.unionbio.com for more information.
About the instrument: Based on similar principles as flow cytometry, the COPAS™ instrument from Union Biometrica provides an automated alternative to manual sorting for increased speed, sensitivity, quantification, and repeatability of experiments. The COPAS BIOSORT instrument analyzes and sorts small model organisms and other objects (from 40-150 microns) on the basis of size, density, and fluorescence signals. A gentle pneumatic sorting mechanism is used to permit the analysis and handling of sensitive organisms and tissues with a high level of recovery and a low level of lethality.
Union Biometrica, Inc. uses "Sorted Events" email newsletters to share information about new, innovative techniques for automated sorting techniques designed for a wide range of applications including: cells, small model organisms, seeds, beads, and other particles (40-1500 microns). For more information, please call (617) 591-1211, e-mail: sales@unionbio.com or visit us on the web at http://www.unionbio.com Union Biometrica, Inc. • 35 Medford Street • Suite 101 • Somerville, MA 02143 • USA
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