Introduction to Large Object Flow Cytometry
Many objects are too large and too fragile for conventional flow cytometry. As an alternate, manual microscopic manipulation of multicellular organisms, large fragile cells and cell clusters, or bead-based assays is tedious and subjective thereby limiting the size and scope of experiments. To fill this void, in 1998 the first COPAS™ (Complex Object Parametric Analyzer and Sorter) was introduced as a tool for high throughput manipulation of C.elegans in drug screening.
Over the next 10 years Union Biometrica expanded the COPAS platform into a family of fully-automated systems for high throughput analysis, sorting / dispensing of large objects ranging from 20–1500 microns in diameter. Each of the four unique COPAS models is engineered around a specific flow cell size and optimized to accommodate different size ranges of small model organisms, large cells / cell clusters, seeds and pollen, as well as large particles such as beads used as micro-carriers or for peptide libraries. By automating time consuming manual sorting processes, the time required for experiments is dramatically reduced, human error is eliminated and new experiments that previously could not be considered are now possible. In addition, digital electronic detection provides increased sensitivity and improved quantitative discrimination.
With the introduction of the BioSorter™ platform, Union Biometrica offers a single system with interchangeable Fluidic and Optics Core Assemblies thereby addressing the need for more flexible, departmental and core laboratory shared instruments.
The BioSorter instrument incorporates the same patented technologies for analysis and gentle sorting of 10 to 1,500 micron objects based on size (TOF / time-of-flight), optical density (EXT / extinction) and by the presence and intensity of fluorescent markers. Both platforms deliver high purity and yield, while maintaining sterility and viability of even large fragile cells.