Union Biometrica Publications

Functional assessment of automatically sorted pancreatic islets using large particle flow cytometry.

2011-09-01T04:00:00Z

Department of Medicine III; Technische Universität Dresden; Dresden, Germany.

Genome-Wide Analysis Reveals a Major Role in Cell Fate Maintenance and an Unexpected Role in Endoreduplication for the Drosophila FoxA Gene Fork Head

2011-06-16T04:00:00Z

Department of Cell Biology, The Johns Hopkins University, School of Medicine, Baltimore, Maryland, United States of America

Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans reveal fat stores using microscopy and flow cytometry approaches

2011-03-18T04:00:00Z

Institute of Human Nutrition and Food Science, Research Group Molecular Prevention, University of Kiel, Kiel, Germany.

Microbial encapsulation in monodisperse hydrogel microspheres enables fast and sensitive phenotypic analyses using flow cytometers

2011-02-28T05:00:00Z

1) Department of Genetics, Universidad de Sevilla, Seville, Spain. 2) Ingeniatrics Tecnologías SL, Spain. 3) Department of Microbiology and Parasitology, Universidad de Sevilla, Seville, Spain. 4) Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Seville, Spain. 5) Biomedal SL, Spain

Microbial encapsulation in monodisperse hydrogel microspheres enables fast and sensitive phenotypic analyses using flow cytometers

2011-02-28T05:00:00Z

1) Department of Genetics, Universidad de Sevilla, Seville, Spain 2) Ingeniatrics Tecnologías SL, Spain 3) Department of Microbiology and Parasitology, Universidad de Sevilla, Seville, Spain 4) Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Seville, Spain 5) Biomedal SL, Spain

A High-Throughput Screen for Tuberculosis Progression

2011-02-16T05:00:00Z

¹ZF-screens B.V., Leiden, The Netherlands, ²Institute of Chemistry, Leiden University, Leiden, The Netherlands, ³Institute of Biology, Leiden University, Leiden, The Netherlands,⁴Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands,^#Contributed equally, E-mail: h.p.spaink@biology.leidenuniv.nl

Heterogeneity of Aspergillus niger in micro-colonies in Liquid Shaken Cultures.

2011-02-01T05:00:00Z

Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Proteome changes of Caenorhabditis elegans upon a Staphylococcus aureus infection

2011-01-05T05:00:00Z

{gamma}-Adducin Stimulates the Thiazide-sensitive NaCl Cotransporter.

2010-12-16T05:00:00Z

*Department of Physiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

[SA-PO2763] y-Adducin Functions as a Novel Regulator of the Thiazide-Sensitive NaCl-Cotransporter

2010-11-20T05:00:00Z

[F-PO1585] Distal Convoluted Tubule (DCT) Specific Transcripts Identified Using DCT Large Scale Sampling

2010-11-19T05:00:00Z

Transgenic Mice: Beyond the Knockout.

2010-11-10T05:00:00Z

1) National Institutes of Health.

Automated High-Content Live Animal Drug Screening Using C. elegans Expressing the Aggregation Prone Serpin a1-antitrypsin Z.

2010-11-01T04:00:00Z

1) Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC and Magee-Womens Hospital Research Institute, Pittsburgh, Pennsylvania, United States of America, 2) Department of Pharmacology and Chemical Biology, University of Pittsburgh Drug Discovery Institute, University of Pittsburgh, Pennsylvania, United States of America

Multiplexed, high-throughput analysis of 3D microtissue suspensions.

2010-10-04T04:00:00Z

Massachusetts Institute of Technology, Cambridge, 02139, USA.

Zebrafish high throughput screening using robotic injection technology

2010-09-02T04:00:00Z

¹Leiden University, Leiden The Netherlands; ²ZF-screens B.V. Leiden, The Netherlands

An antibiotic selection marker for nematode transgenesis.

2010-09-01T04:00:00Z

1) Genome Regulation and Evolution, INSERM U869, Institut Européen de Chimie et Biologie, Pessac, France. 2) Center for Cancer Systems Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. 3) Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. 4) Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. 5) Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.

Serum-free scaled up expansion and differentiation of murine embryonic stem cells to osteoblasts in suspension bioreactors.

2010-08-01T04:00:00Z

Pharmaceutical Production Research Facility (PPRF), Schulich School of Engineering, University of Calgary, 2500 University Drive N.W., Calgary, Alberta, Canada.

Tissue engineered tumor models

2010-08-01T04:00:00Z

Cænorhabditis elegans as a screening tool to find active compounds and targets in rare neuromuscular diseases.

2010-08-01T04:00:00Z

CNRS UMR5534 – Centre de Génétique Moléculaire et Cellulaire, 16 rue Raphaël Dubois – bâtiment Mendel, Campus de La Doua – 69100 Villeurbanne - FRANCE

Zebrafish High-Throughput Screening of Innate Immune Responses.

2010-06-21T04:00:00Z

¹Leiden University, Leiden The Netherlands; ²ZF-screens B.V. Leiden, The Netherlands

Zebrafish are an excellent model for studying the mechanisms of the innate immune defense against pathogens. A high throughput approach is described for a study of the innate immunity in response to bacterial pathogens. The COPAS flow cytometer provided the data analysis in a quick, unbiased and high throughput manner.

Optimizing analysis of high-throughput screens with the UBI Biosort

2010-06-16T04:00:00Z

1) Centre d'Immunologie de Marseille Luminy, Case 906, 13288 Marseille Cedex 9, France, 2) Modul-Bio, Parc Scientifique Luminy Biotech II, Case 935,13288 Marseille Cedex 9

A suite of MATLAB-based computational tools for automated analysis of COPAS Biosort data

2010-06-01T04:00:00Z

Department of Physiology, University of Pennsylvania, Philadelphia, PA, USA

3-nitro-tyrosine as an internal quencher of autofluorescence enhances the compatibility of fluorescence based screening of OBOC combinatorial libraries.

2010-06-01T04:00:00Z

Division of Hematology & Oncology, Department of Internal Medicine, UC Davis Cancer Center, University of California Davis, California, USA

A differential proteomics study of Caenorhabditis elegans infected with Aeromonas hydrophila.

2010-06-01T04:00:00Z

Research Group of Functional Genomics and Proteomics, K.U. Leuven, Naamsestraat 59, 3000 Leuven, Belgium. annelies.bogaerts@bio.kuleuven.be

The fatty acid synthase fasn-1 acts upstream of WNK and Ste20/GCK-VI kinases to modulate anitmicrobial peptide expression in C. elegans epidermis

2010-05-31T04:00:00Z

1) Centre d’Immunologie de Marseille-Luminy; Université de la Méditerranée; Marseille, France; 2) INSERM; U631; Marseille, France; 3) CNRS; UMR6102; Marseille, France; 4)Howard Hughes Medical Institute and Dept of MCDB; University of Colorado at Boulder; Colorado, USA

High-throughput screening and small animal models, where are we?

2010-05-01T04:00:00Z

Université Claude Bernard Lyon, Villeurbanne, France. giacomottojean@gmail.com <giacomottojean@gmail.com>

Reduction of diffusion barriers in isolated rat islets improves survival, but not insulin secretion or transplantation outcome

2010-04-01T04:00:00Z

¹Department of Physical Therapy and Rehabilitation Science; University of Kansas Medical Center; Kansas City, KS USA

⁴Department of Anatomy and Cell Biology; University of Kansas Medical Center; Kansas City, KS USA

²Department of Pharmeceutical Chemistry; University of Kansas; Lawrence, KS USA

³Endocrinology Section; Mercy Hospitals and Clinics; Kansas City, MO USA

A Conserved PMK-1/p38 MAPK Is Required in Caenorhabditis elegans Tissue-specific Immune Response to Yersinia pestis Infection*

2010-02-04T05:00:00Z

1) From the Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710

Accurate MALDI-TOF/TOF sequencing of one-bead-one-compound peptide libraries with application to the identification of multiligand protein affinity agents using in situ click chemistry screening.

2010-01-15T05:00:00Z

Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669

FISH-CS—a rapid method for counting and sorting species of marine zooplankton

2010-01-01T05:00:00Z

1) Marine Science Institute, and 2) Department of Ecology, Evolution and Marine Biology, University of California Santa Barbara, Santa Barbara, California 93106, USA

3) Present address: Bren School of Environmental Resource Management, University of California Santa Barbara, Santa Barbara, California 93106, USA

Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication.

2009-11-20T05:00:00Z

Department of Radiation and Stress Cell Biology, Division of Cell Biology, University Medical Center Groningen, University of Groningen, 9713 AV Groningen, The Netherlands.

Abstract:

Hydroxyurea, a well-known DNA replication inhibitor, induces cell cycle arrest and intact checkpoint functions are required to survive DNA replication stress induced by this genotoxic agent. Perturbed DNA synthesis also results in elevated levels of DNA damage. It is unclear how organisms prevent accumulation of this type of DNA damage that coincides with hampered DNA synthesis. Here, we report the identification of stonewall (stwl) as a novel hydroxyurea-hypersensitive mutant. We demonstrate that Stwl is required to prevent accumulation of DNA damage induced by hydroxyurea; yet, Stwl is not involved in S/M checkpoint regulation. We show that Stwl is a heterochromatin-associated protein with transcription-repressing capacities. In stwl mutants, levels of trimethylated H3K27 and H3K9 (two hallmarks of silent chromatin) are decreased. Our data provide evidence for a Stwl-dependent epigenetic mechanism that is involved in the maintenance of the normal balance between euchromatin and heterochromatin and that is required to prevent accumulation of DNA damage in the presence of DNA replication stress.

Primary DCT Cell Culture Using Large Scale DCT Sampling from Mouse Kidneys

2009-11-06T05:00:00Z

1 Anatomy Inst., Univ.of Zurich, Switzerland; 2 Dpt of Physiology, Radboud Univ. Nijmegen MedicalCentre, Nijmegen, Netherlands; 3 Dpt of Pediatrics, Div. of Nephrology, School of Medicine, Univ. of Utah, Salt Lake City, UT.

LIN-28 and the poly(U) polymerase PUP-2 regulate let-7 microRNA processing in Caenorhabditis elegans.

2009-10-16T04:00:00Z

Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, The Henry Wellcome Building of Cancer and Developmental Biology, Cambridge, UK.

Identification of Antifungal Compounds Active against Candida albicans Using an Improved High-Throughput Caenorhabditis elegans Assay

2009-09-14T04:00:00Z

1 Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America, 2 Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, United States of America,

Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans

2009-08-31T04:00:00Z

High-Throughput Screen for Novel Antimicrobials using a Whole Animal Infection Model

2009-06-29T04:00:00Z

†Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, ‡Department of Molecular Biology, §Center for Computational and Integrative Biology, and Center for Systems Biology, Massachusetts General Hospital,Boston, Massachusetts 02114, _Department of Biology, Northeastern University, Boston, Massachusetts 02115, and_Imaging Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142

Whole-Animal High-Throughput Screens: The C. elegans Model

2009-06-01T04:00:00Z

Spectral Measurements of Large Particles by Flow Cytometry

2009-05-01T04:00:00Z

¹La Jolla Bioengineering Institute, La Jolla CA 92037

²Los Alamos National Laboratory, Los Alamos NM 97545

Antifungal Innate Immunity in C. elegans: PKCdelta Links G Protein Signaling and a Conserved p38 MAPK Cascade.

2009-04-23T04:00:00Z

Novel method for high-throughput colony PCR screening in nanoliter-reactors

2009-02-26T05:00:00Z

1) ETH Zurich, Institute of Process Engineering, BioProcess Laboratory (BPL), 2) ETH Zurich, Institute of Plant Science, Plant Biotechnology, Zurich, Switzerland, 3) Max Planck Institute for Molecular Genetics, Analytics & Computing, Berlin, Germany and 4) Harlan Laboratories Ltd., Environmental Safety and Metabolism, Itingen, Switzerland

Neuroimmune regulation of antimicrobial peptide expression by a noncanonical TGF-beta signaling pathway in Caenorhabditis elegans epidermis.

2009-02-08T05:00:00Z

Negative regulation of Caenorhabditis elegans epidermal damage responses by death-associated protein kinase.

2009-02-01T05:00:00Z

Differentiation of COPAS-sorted non-endocrine pancreatic cells into insulin-positive cells in the mouse.

2009-01-30T05:00:00Z

Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, 1 Joslin Place, Boston, MA, 02215, USA.

Impact of Islet Size on Graft Function

2009-01-25T05:00:00Z

1) University of Kansas Medical Center, Great Plain Diabetes Institute

A High-Throughput Assay To Measure Whole Body Metabolic Rate Using Zebrafish Larvae.

2009-01-25T05:00:00Z

Department of Pediatrics, Gastroenterology¹ and Developmental Biology Sections², Medical College of Wisconsin and Children’s Research Institute, Milwaukee, WI 53226

Whole animal acid secretion can be used as readout for energy metabolism, thus enabling high-throughput chemical and genetic screens for regulators of metabolic rate in a vertebrate. The COPAS sorter was critical for automation.

A C. elegans-based, whole animal, in vivo screen for the identification of antifungal compounds

2008-11-20T05:00:00Z

Division of Infectious Diseases, Massachusetts General Hospital, Gray-Jackson 504, 55 Fruit Street, Boston, Massachusetts 02114, USA.

A Whole-Animal Microplate Assay for Metabolic Rate Using Zebrafish

2008-11-18T05:00:00Z

Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

To study genetics and drugs that impact metabolic rates, whole-animal acid secretion was used as an indicator for energy metabolism. Because of rapid kinetics, COPAS was used for large-scale screening of zebrafish.

Large Scale Sampling of Renal Distal Convoluted Tubules (DCT) by Complex Object Parametric Analysis and Sorting (COPAS)

2008-11-04T05:00:00Z

Inst. of Anatomy, Univ. of Zurich, Zurich, Switzerland; Department of Clinical Neurobiology, Univ. of Heidelberg, Heidelberg, Germany; Unit of Anatomy, Dpt of Medicine, Univ. of Fribourg, Fribourg, Switzerland; Department of Pediatrics, Division of Nephrology, School of Medicine, Univ. of Utah, Salt Lake City, UT

Poster session: J Am Soc Nephrol 19: 2008 ; SA-PO2135

Abstract:

The renal DCT is important for the renal control of Na and K homeostasis. It also contributes to renal acid secretion and calcium and magnesium reabsorption. DCT function is difficult to study as the DCT is a rather short and difficult to identify nephron portion. Its hidden localization in the renal cortical labyrinth makes it also almost inaccessible to direct functional measurements. Recently, the group of R.L. Miller applied large-particle based flow cytometry (Complex Object Parametric Analysis and Sorting COPAS) (AJP Renal Physiol. 2006 Jul.) for large-scale sampling of collecting ducts (CD) using a transgenic mouse expressing GFP in CD. We used the same COPAS technique with a transgenic mouse expressing EGFP in the DCT in order to obtain large quantities of DCTs. Optimization of the COPAS sorting technique allows us to obtain up to 15000 fluorescent DCTs from one single mouse in less than three hours. Microscopical analysis revealed that almost all sorted tubules exhibit green fluorescence. Only occasionally some non-fluorescent tubule segments were attached. Western blot analysis confirmed the significant enrichment of the DCT-specific thiazide sensitive NaCl cotransporter in the sorted samples. Marker proteins for other nephron segments (i.e. NapiIIa - proximal tubule, NKCC2 thick ascending limb, AQP2 connecting tubule/collecting duct) were not detectable. Thus, COPAS allows rapid isolation of rather pure DCT preparations. These preparations will allow us now to study regulatory pathways involved in the DCT functions in vivo and to develop DCT cell culture for further functional testing in vitro.

Rapid Identification and Sorting of Marine Larvae

2008-07-06T04:00:00Z

1) Dept. of Ecology, Evolution and Marine Biology, U. of California, Santa Barbara, CA, USA, 2) Marine Science Institute, U. of California, Santa Barbara, CA, USA. Corresponding author email: hoaglund@lifesci.ucsb.edu

Anti-Fungal Innate Immunity in C. elegans Is Enhanced by Evolutionary Diversification of Antimicrobial Peptides

2008-07-01T04:00:00Z

1 Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, Marseille, France 2 INSERM, U631, Marseille, France 3 CNRS, UMR6102, Marseille, France 4 Department of Animal Evolutionary Ecology, Zoological Institute, University of Tuebingen, Tuebingen, Germany

Isolation of monoclonal microcarriers colonized by fluorescent E. coli

2008-06-16T04:00:00Z

Microencapsulation can be used for high-throughput (HT) processing of bacterial libraries. Ideally, each microcarrier would contain exactly one cell or colony. However, synthesis yields a mixture of capsules containing the desired one cell, multiple cells and empty microcarriers. E. coli cells expressing green fluorescent protein (GFP) were encapsulated in 35 nl Hydrogel carriers. HT sorting procedures by a COAPS sorter were then used to enrich samples to 95% monoclonality.

Published Online: Jun 16 2008 2:41PM; DOI: 10.1002/cyto.a.20597; Cytometry A. 2008 Sep;73(9):788-98.

Acute antibody-mediated complement activation mediates lysis of pancreatic islets cells and may cause tissue loss in clinical islet transplantation.

2008-04-27T04:00:00Z

Department of Oncology, Rudbeck Laboratory, Uppsala, Sweden.

Distinct Innate Immune Responses to Infection and Wounding in the C. elegans Epidermis.

2008-04-08T04:00:00Z

Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, 13288 Marseille cedex 9, France.

Discovery of a cholecystokinin-gastrin-like signaling system in nematodes.

2008-03-13T04:00:00Z

In vivo proteomics in Drosophila melanogaster by tandem affinity purification of protein complexes and analysis by Protein Center software

2008-02-09T05:00:00Z

¹University of Cambridge, Cambride, UK; ²Proxeon, Odense, Denmark

Effects of Genetic Mutations and Chemical Exposures on Caenorhabditis elegans Feeding: Evaluation of a Novel, High-Throughput Screening Assay

2007-12-05T05:00:00Z

1 Laboratory of Molecular Toxicology, National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, United States of America 2 Nicholas School of the Environment and Earth Sciences, Duke University, Durham, North Carolina, United States of America

Abstract
Government agencies have defined a need to reduce, refine or replace current mammalian-based bioassays with testing methods that use alternative species. Invertebrate species, such as Caenorhabditis elegans, provide an attractive option because of their short life cycles, inexpensive maintenance, and high degree of evolutionary conservation with higher eukaryotes. The C. elegans pharynx is a favorable model for studying neuromuscular function, and the effects of chemicals on neuromuscular activity, i.e., feeding. Current feeding methodologies, however, are labor intensive and only semi-quantitative.

Here a high-throughput assay is described that uses flow cytometry to measure C. elegans feeding by determining the size and intestinal fluorescence of hundreds of nematodes after exposure to fluorescent-labeled microspheres. . . .

A global analysis of genetic interactions in Caenorhabditis elegans

2007-09-26T04:00:00Z

Addresses: *Department of Medical Genetics and Microbiology, The Terrence Donnelly Centre for Cellular and Biomolecular Research, 160 College St, University of Toronto, Toronto, ON, M5S 3E1, Canada. ^†Collaborative Program in Developmental Biology, University of Toronto, Toronto, ON, M5S 3E1, Canada. ^‡Department of Biomolecular Engineering, 1156 High Street, Mail Stop SOE2, University of California, Santa Cruz, CA 95064, USA.

Correspondence: Peter J Roy. Email: peter.roy@utoronto.ca; Joshua M Stuart. Email: jstuart@soe.ucsc.edu

16th International C. elegans Conference 2007 June 27 - 30

2007-06-27T04:00:00Z

Click on the titles of the following posters to view an abstract:

Genome-scale analysis of in vivo spatiotemporal promoter activity in C. elegans.
Denis Dupuy, Nicolas Bertin, César Hidalgo, Kavitha Venkatesan, Domena Tu, David Lee, Nenad Svrzikapa, Anne-Ruxandra Carvunis, Rock Pulak, Ian Hope, John Reece-Hoyes, Rebecca Hunt-Newbury, Ryan Viveiros, William Mohler, Murat Tasan, Frederick Roth, Donald Moerman, Albert-László Barabási, David Baillie, Marc Vidal.

Genetic dissection of the transcriptional response to wounding and fungal infection.
Nathalie Pujol, C. Leopold Kurz, Sophie Cypowyj, Daniel Wong, Andrew Chisholm, Jonathan Ewbank.

A high throughput screen for novel anti-infective compounds.
Terence I. Moy, Annie L. Conery, Anne E. Carpenter, Anthony R. Ball, Kim Lewis, Frederick M. Ausubel.

Characterization of the role of peni( fr8) in innate immunity against fungal infection.
Kwang-Zin Lee, Nathalie Pujol, Andrew Chisholm, Jonathan Ewbank.

Automated drug screening and chemical genetics in C. elegans models of rare diseases.
Jean Giacomotto, Maité Carre-Pierrat, Laurent Segalat.

Isolation and characterization of C. elegans mutants deficient in PMK-1-dependent immunity.
Robert Shivers, Tristan Kooistra, Bethany Redding, Dennis H. Kim.

A Sodium: Neurotransmitter symporter Family protein required for the induction of antimicrobial peptides in C. elegans.
Katja Ziegler, Jonathan Ewbank, Nathalie Pujol.

High-throughput chemical screening using C. elegans growth and development.
Windy A. Boyd, Sandra J. McBride, Marjolein V. Smith, Grace E. Kissling, Julie R. Rice, Daniel W. Snyder, Christopher J. Portier, Jonathan H. Freedman.

Using a C. elegans feeding response assay in high-throughput chemical and genetic screening.
Windy A. Boyd, Sandra J. McBride, Jonathan H. Freedman.

Genetic dissection of Spinal Muscular Atrophy in Drosophila and C. elegans.
Jevede D. Harris, Tom Barsby, Amy K. Walker, Anne C. Hart. Gelsomino, Paolo Bazzicalupo.

Large scale genetic screens for cell fate mutants using worm sorter technology.
Vincent Bertrand, Nuria Flames, Maria Doitsidou, Richard Poole, Janine Recio, Oliver Hobert.

Semi-automated genetic screen for temperature sensitive mutations that abolish the early embryonic expression of the hox gene ceh-13.
Stephan Knierer, Adrian Streit.

The C. elegans dysferlin homolog fer-1 is expressed in muscle.
Todd Lamitina, Olga Lozynska, Tejvir Khurana.

Toward the development of a database for the storage and integration of COPAS™ BioSorter™ data.
Lisa R. Matthews, Philippe Vaglio, Jonathan Ewbank.

A French functional genomics platform.
Yohann Duverger, Sarah Scaglione, Daniel Wong, Jérôme Reboul, Jonathan Ewbank.

A semi-automated high-throughput approach to the generation of transposon insertion mutants.
Yohann Duverger, Jérôme Belougne, Sarah Scaglione, Dominique Brandli, Christophe Beclin, Jonathan Ewbank.

A green light to expression in time and space.

2007-06-25T04:00:00Z

Genome-scale analysis of in vivo spatiotemporal promoter activity in Caenorhabditis elegans.

2007-05-07T04:00:00Z

¹Center for Cancer Systems Biology (CCSB), and Department of Cancer Biology, Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA. ²Centre de Recherche en Biochimie Macromole´culaire, Centre National de la Recherche Scientifique FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France. ³Center for Complex Network Research, Department of Physics, University of Notre Dame, 225 Nieuwland Science Hall, Notre Dame, Indiana 46556, USA. 4Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada. ⁵Union Biometrica, 84 October Hill Road, Holliston, Massachusetts 01746, USA. ⁶Institute of Integrative and Comparative Biology, University of Leeds, Clarendon Way, Leeds LS2 9JT, West Yorkshire, UK. ⁷Department of Zoology, The University of British Columbia, 6270 University Boulevard, Vancouver, British Columbia V6T 1Z4, Canada. ⁸Department of Genetics and Developmental Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030, USA. ⁹Center for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA. ¹⁰These authors contributed equally to this work. Correspondence should be addressed to M.V. (marc_vidal@dfci.harvard.edu), D.B. (baillie@sfu.ca) or A.-L.B. (alb@nd.edu).

Genome-wide mapping and characterisation of protein expression and interaction in Drosophila melanogaster, using a hybrid piggyBac/P-element YFP gene trap system with tandem affinity tags

2007-03-07T05:00:00Z

1. Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH. UK. 2. The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2. 1QN. 3. Cambridge Systems Biology Centre, Tennis Court Road, Cambridge, CB2 1QR

Exploring Strategies for Protein Trapping in Drosophila

2007-03-01T05:00:00Z

^* Department of Genetics, ^** Department of Cell Biology and ^†Department of Molecular, Cellular and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut 06520, ^‡Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218 and ^§Department of Genome Biology, Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720

The Carnegie Protein Trap Library: A Versatile Tool for Drosophila Developmental Studies

2007-03-01T05:00:00Z

^*Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, ^†Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 and ^‡Department of Genome Biology, Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720

Aminodeoxychorismate Synthase Inhibitors from One-Bead One-Compound Combinatorial Libraries: “Staged” Inhibitor Design

2006-11-10T05:00:00Z

^†Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, ^‡Department of Chemistry, Central Connecticut State University, 1615 Stanley Street, New Britain, Connecticut 06050, and ^§Department of Internal Medicine, Division of Hematology and Oncology, UC Davis Cancer Center, 4501 X Street, Sacramento, California 95817

A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans

2006-11-03T04:00:00Z

¹Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, 13288 Marseille cedex 9, France, ²INSERM, U631, 13288 Marseille, France, ³CNRS, UMR6102, 13288 Marseille, France and ⁴CNRS, Institut de Biologie du Développement de Marseille-Luminy, Marseille, France.

Received September 5, 2006; Revised October 18, 2006; Accepted November 3, 2006.

Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo

2006-11-01T04:00:00Z

¹School of Biomedical Science and ²Medical Research Institute, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. ³Department of Physiology, Tokyo Women’s Medical University School of Medicine, Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. ⁴CREST, JST, Hon-cho, Kawaguchi, Saitama 332-0012, Japan.

A small-molecule screen in C. elegans yields a new calcium channel antagonist.

2006-05-04T04:00:00Z

Department of Medical Genetics and Microbiology, and The Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.

Genome-wide RNAi screening identifies protein damage as a regulator of osmoprotective gene expression.

2006-04-12T04:00:00Z

Departments of Anesthesiology and Pharmacology, Vanderbilt University, T4208 Medical Center North, 1161 21st Avenue South, Nashville, TN 37232.

The detection, stabilization, and repair of stress-induced damage are essential requirements for cellular life. All cells respond to osmotic stress-induced water loss with increased expression of genes that mediate accumulation of organic osmolytes, solutes that function as chemical chaperones and restore osmotic homeostasis. The signals and signaling mechanisms that regulate osmoprotective gene expression in animal cells are poorly understood. Here, we show that gpdh-1 and gpdh-2, genes that mediate the accumulation of the organic osmolyte glycerol, are essential for survival of the nematode Caenorhabditis elegans during osmotic stress. Expression of GFP driven by the gpdh-1 promoter (Pgpdh-1::GFP) is detected only during hypertonic stress but is not induced by other stressors. Using Pgpdh-1::GFP expression as a phenotype, we screened approximately 16,000 genes by RNAi feeding and identified 122 that cause constitutive activation of gpdh-1 expression and glycerol accumulation. Many of these genes function to regulate protein translation and cotranslational protein folding and to target and degrade denatured proteins, suggesting that the accumulation of misfolded proteins functions as a signal to activate osmoprotective gene expression and organic osmolyte accumulation in animal cells. Consistent with this hypothesis, 73% of these protein-homeostasis genes have been shown to slow age-dependent protein aggregation in C. elegans. Because diverse environmental stressors and numerous disease states result in protein misfolding, mechanisms must exist that discriminate between osmotically induced and other forms of stress-induced protein damage. Our findings provide a foundation for understanding how these damage-selectivity mechanisms function.

PMID: 16880390 [PubMed - as supplied by publisher]

American Journal of Physiology: Renal Physiology, Automated Method for the Isolation of Collecting Ducts

2006-02-07T05:00:00Z

1) Department of Pediatrics, Division of Nephrology, University of Utah, School of Medicine, Salt Lake City, UT, USA. 2) Department of Human Genetics, University of Utah, School of Medicine, Salt Lake City, UT, USA.

Flow Cytometry for "Large Objects" (40-1,000 microns): HTS of bead-based combi-chem libraries, model organisms (C. elegans, Drosophila, zebra fish), Arabidopsis seeds, pollen, etc., #33.

2006-01-05T05:00:00Z

SIRT1 Top 40 Hits: Use of One-Bead, One-Compound Acetyl-Peptide Libraries and Quantum Dots to Probe Deacetylase Specificity.

2005-12-13T05:00:00Z

Departments of Biomolecular Chemistry and Chemistry, University of Wisconsin, Madison, Wisconsin 53706

Drug Discovery & Development, "Characterizing Targets in Model Organisms"

2005-11-01T04:00:00Z

An Anopheles transgenic sexing strain for vector control

2005-10-09T04:00:00Z

1) Imperial College London, Department of Biological Sciences, Imperial College Road, London SW7 2AZ, UK.
2) These authors contributed equally to this work.

Validation of Large Particle Flow Cytometry for the Analysis and Sorting of Intact Pancreatic Islets.

2005-09-27T04:00:00Z

1) Department of Surgery, University of Wisconsin-Madison, Madison, WI. 2) Department of Surgery, University of Leipzig, Germany

Multiparametric analysis of neuromast hair cells in intact early larvae using a large particle sorter.

2005-09-09T04:00:00Z

1) Union Biometrica Inc., 84 October Hill Rd, Holliston, MA 01746 USA

A stress-sensitive reporter predicts longevity in isogenic populations of Caenorhabditis elegans

2005-07-24T04:00:00Z

1) Institute for Behavioral Genetics, University of Colorado at Boulder, Box 447, Boulder, Colorado 80309, USA. 2) Max Planck Institute for Demographic Research, Konrad-Zuse-Strasse 1, D-18057, Rostock, Germany. 3) Department of Integrative Biology, University of Colorado at Boulder, Box 447, Boulder, Colorado 80309, USA. 4) These authors contributed equally to this work. Published online: 24 July 2005.

A non-biased, in vivo genetic screen for genes that protect against necrosis. (Poster 758A)

2005-06-25T04:00:00Z

Dept Mol Biol & Biochem, Rutgers Univ, Piscataway, NJ.

Multi-parameter axial profiling of transgenic C. elegans expressing fluorescent proteins from various cell-specific, tissue specific and developmentally regulated promoters. (Poster 1172A)

2005-06-25T04:00:00Z

1) Union Biometrica, Holliston, MA; 2) Division of Biology, Kansas State University, Manhattan, Kansas.

A French functional genomics platform. (Poster 1294C)

2005-06-25T04:00:00Z

1) Centre d’Immunologie de Marseille-Luminy, INSERM/CNRS/Université de la Méditerranée, Marseille, France; 2) INSERM UMR 599, Institut Paoli Calmette, Marseille, France.

The C. elegans Localizome Project: a beginning. (Plenary Session 212)

2005-06-25T04:00:00Z

1) Center for Cancer Systems Biology (CCSB)/Dana-Farber Cancer Institute/Harvard Medical school, Boston, MA; 2) Union Biometrica, Holliston, MA; 3) University of Leeds, Leeds, UK; 4) Department Molecular Biology and Biochemistry, Simon Fraser University, Vancouver, BC; 5) Department of Zoology, The University of British Columbia, BC; 6) Dept. of Genetics and Developmental Biology University of Connecticut Health Center, Farmington, CT.

Identification of new innate immunity genes. (Parallel Session 294)

2005-06-25T04:00:00Z

Centre d’Immunologie de Marseille-Luminy, INSERM/CNRS/Univ. de la Méditerranée, Marseille, France.

Stochastic Effects Make a Big Difference in How Long You Will Live (If You Are a Worm). (Plenary session 311)

2005-06-25T04:00:00Z

Inst Behavioral Genetics, University of Colorado, Boulder, CO.

A novel method of quantifying C. elegans feeding. (Poster 408B)

2005-06-25T04:00:00Z

Nicholas School of the Environment and Earth Sciences, Duke University, Durham, NC.

Development of medium-throughput toxicity screens using C. elegans. (Poster 409C)

2005-06-25T04:00:00Z

Nicholas School of the Environment and Earth Sciences, Duke University, Durham, NC.

Studies of Interactions between PAR Proteins. (Poster 1105C)

2005-06-25T04:00:00Z

1) Dept Molecular Biol & Genetics, Cornell Univ, Ithaca, NY; 2) Union Biometrica, Holliston, MA; 3) Department of Morphoregulaltion, Institute for Frontier Medical Sciences, Kyoto Univ, Kyoto, Japan.

Multicellular Cytometric Analyses and Sorting on Stem Cell Clusters and Embryonic Stem Cell Clusters (Poster)

2005-06-23T04:00:00Z

1) Union Biometrica Inc., Holliston, MA 01746 USA; 2) Department of Comparative Medicine, University of Washington, Seattle, WA, USA

A new seed-based assay for meiotic recombination in Arabidopsis thaliana

2005-05-23T04:00:00Z

Meiotic recombination is a fundamental biological process that plays a central role in the evolution and breeding of plants. We have developed a new seed-based assay for meiotic recombination in Arabidopsis. The assay is based on the transformation of green and red fluorescent markers expressed under a seed-specific promoter. A total of 74 T-DNA markers were isolated, sequenced and mapped both physically and genetically. Lines containing red and green markers that map 1–20 cM apart were crossed to produce tester lines with the two markers linked in cis yielding seeds that fluoresced both in red and green. We show that these lines can be used for efficient scoring of recombinant types (red only or green only fluorescing seeds) in a seed population derived from a test cross (backcross) or self-pollination. Two tester lines that were characterized during several generations of backcross and self-pollination, one in the background of ecotype Landsberg and one in the ecotype Columbia, are described. We discuss the number of plants and seeds to be scored in order to obtain reliable and reproducible crossing over rate values. This assay offers a relatively high-throughput method, with the benefit of seed markers (similar to the maize classical genetic markers) combined with the advantages of Arabidopsis. It advances the prospect to better understand the factors that affect the rate of meiotic crossover in plants and to stimulate this process for more efficient breeding and mapping.

(1) Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, and (2) Department of Genetics, Institute for Molecular Cell Biology, Vrije Universiteit, de Boelelaan 1087,1081 HV, Amsterdam, The Netherlands

DOI:10.1111/j.1365-313X.2005.02466.x

Large Particle Flow Cytometry as a New Automated Method of Islet Yield Determination (Poster PS-031)

2005-05-21T04:00:00Z

1) Surgery, University of Wisconsin-Madison, Madison, WI, USA

Rapid and Sensitive Assessment of Human Islet Viability by Multiparameter Flow Cytometry on Intact and Dissociated Islets (Platform Session OP-139)

2005-05-21T04:00:00Z

1) Surgery, University of Wisconsin-Madison, Madison, WI, USA

Large Particle Flow Cytometry as a New Automated Method of Islet Yield Determination (Poster PS-031)

2005-05-04T04:00:00Z

¹Surgery, University of Wisconsin-Madison, Madison, WI, USA

Rapid and Sensitive Assessment of Human Islet Viability by Multiparameter Flow Cytometry on Intact and Dissociated Islets (Platform Session OP-139)

2005-05-04T04:00:00Z

¹Surgery, University of Wisconsin-Madison, Madison, WI, USA

Genetic analysis of osmotically regulated gene expression in C. elegans

2005-04-02T04:00:00Z

Department of Anesthesiology, Vanderbilt University, 1161 21st Avenue South, MCN T4208, Nashville, TN, 37232.

Microarray-based screens to identify genes specific to the fusion-competent myoblasts. (874A) (Poster)

2005-03-30T04:00:00Z

Stowers Institute for Medical Research, Kansas City, MO 64110.

Gene expression profiling during gliogenesis in Drosophila.

2005-03-30T04:00:00Z

Institute of Genetics, University of Mainz, Germany

High throughput collection of Drosophila embryos for homozygous lethal mutants based on deformed driven YFP expression

2005-03-30T04:00:00Z

1) Union Biometrica Inc., 84 October Hill Road, Holliston, MA 01746 USA; 2) Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.

XNP-1/ATR-X acts with RB, HP1 and the NuRD complex during larval development in C. elegans

2005-02-01T05:00:00Z

^aINSERM U491, Faculte´ de Me´decine la Timone, 27, Bd Jean Moulin, 13385 Marseille Cedex 5, France ^bCentre d'Immunologie de Marseille Luminy, INSERM/CNRS/Universite´ de la Me´diterrane´e, Campus Luminy Case 906, 13288 Marseille Cedex 9, France.

Diversity and specificity in the interaction between Caenorhabditis elegans and the pathogen Serratia marcescens

2004-11-22T05:00:00Z

1) Department of Evolutionary Biology, Institute for Animal Evolution and Ecology, Westphalian Wilhelms-University, Hüfferstr. 1, 48149 Münster, Germany and 2) Centre d'Immunologie de Marseille Luminy, INSERM/CNRS/Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France.

Published: 22 November 2004. doi:10.1186/1471-2148-4-49.

High-throughput sublethal toxicity tests using the nematode Caenorhabditis elegans.

2004-11-14T05:00:00Z

1) Duke University, Durham, NC, USA

Automated assays to study longevity in C. elegans

2004-10-20T04:00:00Z

Maren Hertweck(1), and Ralf Baumeister, Bio3/Bioinformatics and Molecular Genetics, Albert-Ludwigs University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.

An Unlikely Star of Science: Jonathan Freedman Looks to Microscopic Roundworms to Document the Effects of Toxic Chemicals.

2004-09-21T04:00:00Z

OBOC Small-Molecule Combinatorial Library Encoded by Halogenated Mass-Tags

2004-09-17T04:00:00Z

Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, California 95616-5295, and Division of Hematology and Oncology, Department of Internal Medicine, University of California-Davis Cancer Center, 4501 X Street, Sacramento, California 95817

Go With the Flow: Sorting Cells and Other Small Things

2004-09-01T04:00:00Z

Long-term effects of sterol depletion in C. elegans: Sterol content of synchronized wild-type and mutant populations.

2004-08-16T04:00:00Z

^*Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854; ^†Department of Veterans Affairs New Jersey Health Care System, 385 Tremont Avenue, East Orange, NJ 07018; ^§Department of Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07101

Chapter 4: Practical applications of RNAi in C. elegans, "RNA interference: From Basic Biology to Drug Development,"

2004-06-21T04:00:00Z

Discusses the use of the COPAS BIOSORT to score RNAi phenotypes complete with illustrations and pictures. SUMMER, 2004

Flow cytometry for isolation and optical analysis of embryoid bodies from cultured mouse embryonic stem cells.

2004-06-10T04:00:00Z

1) Axiogenesis, Joseph-Stelzmann-Str. 50, Cologne, Germany, 2) Union Biometrica -Cipalstraat 3, Geel, Belgium, 3)Union Biometrica Inc., Holliston, MA 01746 USA

Combinatorial library of peptidotriazoles: identification of [1,2,3]-triazole inhibitors against a recombinant Leishmania mexicana cysteine protease.

2004-05-01T04:00:00Z

Center for Solid-Phase Organic Combinatorial Chemistry, Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.

TLR-independent control of innate immunity in Caenorhabditis elegans by the TIR domain adaptor protein TIR-1, an ortholog of human SARM

2004-03-28T04:00:00Z

1) Centre d'Immunologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale/Centre National de la Recherche Scientifique/Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France. 2) Institut National de la Santé et de la Recherche Médicale U119, Institut Paoli Calmette, 13009 Marseille, France. 3) Institut de Biologie Moléculaire et Cellulaire, UPR 9022, Centre National de la Recherche Scientifique, 15 rue Descartes, 67084 Strasbourg Cedex, France. 4) Centre de Biochimie Structurale, Centre National de la Recherche Scientifique UMR 5048, Institut National de la Santé et de la Recherche Médicale UMR 554, Université de Montpellier 1, 29, rue de Navacelles, 34090 Montpellier Cedex, France. 5) National Institute of Genetics, Mishima 411, Japan.

Sex-specific GFP-expression in Drosophila embryos and sorting by COPAS flow cytometry technique (#615C).

2004-03-24T04:00:00Z

1) Union Biometrica Inc (Holliston, MA USA), 2) Department of Molecular Biology, Princeton University (Princeton, NJ USA).

Drosophila Fluorescent-Imaginal Disc Analysis and Sorting by COPAS Flow Cytometry Technique (412A).

2004-03-24T04:00:00Z

1) Max Planck Institute, CBG, Susan Eaton Lab (Dresden, Germany), 2) Biozentrum, Walter Gehring lab (Basel, Switzerland), 3) Union Biometrica (Geel, Belgium), 4) Union Biometrica Inc. (Holliston, MA, USA).

Drosophila Fluorescent-Imaginal Disc Analysis and Sorting by COPAS Flow Cytometry Technique (412A)

2004-03-24T04:00:00Z

Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster.

2004-01-01T05:00:00Z

Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA

1) Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA 2) Department of Genetics, and 3) Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520-8005, USA

Model Organisms in Drug Discovery (2003): Chapter 3: Caenorhabditis elegans Functional Genomics in Drug Discovery: Expanding Paradigms.

2003-12-31T05:00:00Z

Devgen NV, Technologiepark 9, B-9052 Ghent-Zwijnaarde, Belgium

Published Online: 05 Jan 2005
Print ISBN: 0470848936; Online ISBN: 0470014067

A worm's life [Med Sci Paris]

2003-12-28T05:00:00Z

Centre d'Immunologie de Marseille Luminy, Cnrs UMR 6102, Inserm U. 136, Universite de la Mediterranee, Case 906, 13288 Marseille Cedex 09, France.

Solid Phase Combinatorial Library of 1,3-Azole Containing Peptides for the Discovery of Matrix Metallo Proteinase Inhibitors

2003-12-08T05:00:00Z

1) Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark, 2) Nordic Bioscience A/S, Herlev Hovedgade 207, DK-2730 Herlev, Denmark.

Use of the COPAS Biosort in a hi-throughput screen for genes that regulate lifespan.

2003-08-11T04:00:00Z

Department of Biochemistry and Biophysics, University of California, San Francisco.

A report on the 62nd Annual Meeting for the Society for Developmental Biology (SDB), Boston, USA, 30 July to 3 August 2003, "Developments in developmental genomics."

2003-07-30T04:00:00Z

1) Department of Biochemistry and Biophysics, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA; 2) Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA

New Technology Automates Sorting of Large, Bead-based CombiChem Libraries, P127.

2003-07-19T04:00:00Z

Union Biometrica-(Geel, BE¹, Holliston, MA USA³), Utrecht University² (Utrecht, NL).

From synthetic receptors towards synthetic antibodies using combinatorial chemistry, IN4.

2003-06-29T04:00:00Z

Department of Medicinal Chemistry, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, (Utrecht, The Netherlands).

Protein affinity profiling using solid-phase combinatorial libraries, O19.

2003-06-29T04:00:00Z

Carlsberg Biosectro, Valby (Denmark).

Bead-Based assays using COPAS™ Flow Sorting, P16.

2003-06-29T04:00:00Z

Union Biometrica-(Geel, BE¹, Holliston, MA USA³), Utrecht University² (Utrecht, NL).

Imaging strategies and data types for genome-wide comparison and pattern-matching of GFP expression patterns: GLO-Worm, Program #41.

2003-06-29T04:00:00Z

Genetics and Dev. Bio., UConn Health Center, Farmington USA.

Isolation of Long-Lived Individuals Within an Isogenic Population, Program # 145.

2003-06-29T04:00:00Z

Institute for Behavioral Genetics, University of Colorado at Boulder, Boulder, CO USA.

Anti-fungal innate immune defence in C. elegans, Program #287.

2003-06-29T04:00:00Z

1) CIML, Marseille, France; 2) IBMC, Strasbourg, France; 3) CBS, Montpellier, France; 4) NIG, Mishima, Japan.

GFP screens for regulators of motor neuron differentiation, Program #520A.

2003-06-29T04:00:00Z

Neuroscience Program Cell and Developmental Biology, Vanderbilt University, Nashville, TN USA.

Applications of the COPAS (Complex Object Parametric Analysis and Sorting) Biosort to functional genomic studies, Program #1111A.

2003-06-29T04:00:00Z

The Wellcome Trust Sanger Institute, Cambridge, UK.

Mos1 mutagenesis and the Union Biometrica worm sorter: complementary tools for a genetic screen for worms that resist bacterial infection, Program #314B.

2003-06-29T04:00:00Z

CIML, Marseille, FRANCE.

A high throughput screening method to detect youthful nematodes, Program #356B.

2003-06-29T04:00:00Z

Molecular Biology and Biochem, Rutgers University, Piscataway, NJ USA.

Measurement and analysis of stress induced responses in transgenic Caenorhabditis elegans, Program #389B.

2003-06-29T04:00:00Z

Union Biometrica, Holliston, MA USA.

Developing an automating worm-based screen for bacterial virulence factors using the Union Biometrica sorter, Program #312C.

2003-06-29T04:00:00Z

CIML, Marseille, FRANCE.

Genetic analysis of IFT and ARPKD: Isolating osm-5 suppressors, Program #510C.

2003-06-29T04:00:00Z

1) Laboratory of Genetics, University of Wisconsin, Madison, WI USA; 2) Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT USA; 3) School of Pharmacy, University of Wisconsin, Madison, WI, USA.

An Automationed High-Throughput Assay for Survival of the Nematode Caenorhabditis elegans

2003-05-23T04:00:00Z

Buck Institute, Novato, CA (USA). PII: S0891-5849(03)00328-9. SEPTEMBER, 2003

Flow Cytometry: Cytometers are getting smaller, cheaper, faster, and better

2003-05-05T04:00:00Z

Caenorhabditis elegans: an emerging genetic model for the study of innate immunity

2003-05-01T04:00:00Z

Centre d'Immunologie de Marseille Luminy, INSERM/CNRS/Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France.

Bead Based Assays Using COPAS Flow Sorting, Practical Session

2003-04-09T04:00:00Z

Union Biometrica, Geel, Belgium.

Cytometric Sorting of Pinaceae Pollen and its Implications for Radiocarbon Dating and Stable Isotope Analyses.

2003-04-03T04:00:00Z

(1) Department of Geography, UC Berkeley, Berkeley CA, (2) Department of Earth and Planetary Science, UC Berkeley, Berkeley CA, (3) Union Biometrica, Holliston, MA.

Novel High-Throughput Screening Technologies Fluorescence, Bioluminescence & Flow Cytometry Aid Drug Discovery

2003-03-15T04:00:00Z

Instrumentation for analysis and sorting of fluorescence patterns in transgenic Drosophila embryos, #1001B.

2003-03-05T05:00:00Z

Life Sciences, Union Biometrica, Inc. (Holliston, MA.)

New technology automates sorting of large, bead--based combinatorial chemistry libraries, #87.

2003-02-01T05:00:00Z

Union Biometrica 1, 3, (Geel, BE 1; Holliston, MA 3), Utrecht University (Utrecht, NL 2)

Binding site for p120/-catenin is not required for Drosophila E-cadherin function in vivo.

2003-01-27T05:00:00Z

European Molecular Biology Laboratory, 69117 Heidelberg, Germany

Measurement and analysis of stress induced responses in transgenic Caenorhabditis elegans, #L340.

2002-12-14T05:00:00Z

Life Sciences, Union Biometrica, Inc., Holliston, MA.

Assays for Bead-Based Technologies Using COPAS™ Flow Sorting, Session 3, Abstract 11.

2002-10-20T04:00:00Z

Union Biometrica-ESO 1, (Geel, BE), Union Biometrica 3, (Holliston, MA) Utrecht University 2 (Utrecht, NL)

The one-bead two-compound assay for solid-phase screening of Combinatorial libraries.

2002-09-16T04:00:00Z

Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.

Drug Discovery - Automated Drug Screening Using Zebrafish: COPAS XL Allows for Increased Throughput

2002-09-01T04:00:00Z

The principles of scale space applied to structure and colour in light microscopy

2002-09-01T04:00:00Z

GFP screens for regulators of motor neuron differentiation, #242.

2002-08-10T04:00:00Z

Dept. of Cell and Developmental Biology, Vanderbilt University Med. Ctr., Nashville, TN

Check out my Profile! Isolation of chemotaxis defective mutants with altered str-1 expression levels using automated, high-sensitivity fluorescence Profiling, #12

2002-08-10T04:00:00Z

1) Union Biometrica, Inc., 35 Medford St, Holliston, MA 01746, 2) Union Biometrica, GMBH, Cipalstraat 3, B-2440, Geel, Belgium

The application of scale space and the spatial color model in microscopy.

2002-07-09T04:00:00Z

* Union Biometrica N.V., European Scientific Operations, Geel, Belgium. ** ISIS, Faculty of Science, University of Amsterdam, Amsterdam, the Netherlands. *** TU Eindhoven, Faculty Biomedical Technology, Biomedical Imageprocessing, Den Dolech, Eindhoven, the Netherlands

The Multi-Mode Mosaic framework for automated microscopy and analysis.

2002-07-09T04:00:00Z

* Union Biometrica N.V., European Scientific Operations. ** ISIS, Faculty of Science, University of Amsterdam, Amsterdam, the Netherlands

Suppressors of gon-1, #626415

2002-06-28T04:00:00Z

1) Department of Genetics, University of Wisconsin-Madison, Madison, WI; 2) Department of Biochemistry, University of Wisconsin-Madison, Madison, WI; 3) Howard Hughes Medical Institute, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI

Isolation of chemotaxis defective mutants with altered str-1 expression levels using automated, high-sensitivity fluorescence profiling, #496373.

2002-06-28T04:00:00Z

Union Biometrica, Inc., Holliston, MA.

Application of linear scale space and the spatial color model in microscopy

2002-06-24T04:00:00Z

* Union Biometrica N.V., European Scientific Operations, Cipalstraat 3, B-2440 Geel, Belgium, ** Intelligent Sensory Information Systems, Faculty of Science, UvA, Amsterdam, The Netherlands, *** BioMedische Technologie, Technische Universiteit Eindhoven, Eindhoven, The Netherlands.

Post-embryonic Developmental Expression Chronograms: a new functional genomics data type generated using a nematode fluorescence sorting system, #178.

2002-06-14T04:00:00Z

1) University of Connecticut Health Center, Farmington CT, 2) Union Biometrica, Holliston MA

Check out my Profile! Isolation of mutants with altered str-1 expression levels using automated, high-sensitivity fluorescence profiling, #112.

2002-06-14T04:00:00Z

Union Biometrica, Inc., Holliston, MA

Multi-parameter & Dual Color Fluorescence Analysis and Flow Sorting of C. elegans, #199.

2002-06-14T04:00:00Z

Union Biometrica, Inc., Holliston, MA

At the higher end of High Content Screening: The MIAS HTS imaging device, #82.

2002-05-27T04:00:00Z

Union Biometrica, Cipalstraat 3, B-2440 Geel, Belgium

Enhanced Analytical Performance of the C. elegans Flow Sorter COPAS BIOSORT: Automated Re-analysis of Populations in Multi-well Plates and Reading of Axially Distributed Positional Fluorescent Signals, #32.

2002-05-18T04:00:00Z

Union Biometrica Inc. & European Scientific Operations(*)

Enhanced One-step Nematode Recognition on micrographs of Living C. elegans Cultures in 384-well Plates using Linear Scale Space Mathematics.

2002-05-18T04:00:00Z

*Union Biometrica, European Scientific Operations, Geel, Belgium; **Intelligent Sensory Information Systems, UvA, Amsterdam, the Netherlands.

Multi-parametric & dual color fluorescent analysis and flow sorting of C. elegans.

2002-05-18T04:00:00Z

*Union Biometrica, European Scientific Operations, Geel, Belgium; **Union Biometrica, Holliston, MA, USA.

High Throughput Protein Trapping in Drosophila, #982A.

2002-04-10T04:00:00Z

Dept MCDB, Yale Univ, New Haven, CT; 2) Institute of Molecular and Cell Biology, King's College London, Guy's Hospital, London, UK; 3) Dept Genetics, Yale Univ, New Haven, CT; 4) Dept Cell Biology, Yale Univ, New Haven, CT.

Using Protein Traps to Study Genes Expressed during Oogenesis, #608B.

2002-04-10T04:00:00Z

1) Genetics, Yale University, New Haven, CT; 2) MRC Centre for Developmental Neurobiology, King's College London, New Hunts House, Guy's Hospital, London, UK.

A rapid and efficient approach to vital enhancer trap screening in Drosophila embryos, #143.

2002-04-10T04:00:00Z

Howard Hughes Medical Institute, Brigham and Women's Hospital and Harvard Medical School, Boston, MA; 2) Union Biometrica, Inc., Holliston, MA.

The World of Small Model Organisms Just Got Larger: Companies have identified disease pathways that can be targeted for drug discovery using worms, fruit flies, and zebrafish

2002-03-01T05:00:00Z

Firms Creating More Focused Compound Libraries, Saving Time and Money in Drug Discovery Research

2001-11-15T05:00:00Z

Fully Automated Instrumentation for Analysis in C elegans, #1089.

2001-06-22T04:00:00Z

Union Biometrica, Inc., Holliston, MA.

Application of the COPAS (Complex Object Parametric Analysis and Sorting) dispenser to functional genomic studies, #1096.

2001-06-22T04:00:00Z

The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK

Automated Sorting of C. elegans Muv Mutants According to Pseudovulva Number, #1097.

2001-06-22T04:00:00Z

Union Biometrica, Inc., Holliston, MA

Genetic Analysis of Digestive Physiology Using Fluorescent Phosolipid Reporters

2001-05-18T04:00:00Z

Living the high (throughput) life: HTS looks for rewards from the use of cellular assays

2001-05-01T04:00:00Z

Novel Methods for Analysis of Solid Phase Combinatorial Libraries on PEG-based Resins, #1134.

2001-05-01T04:00:00Z

SPOCC-center, Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.

Union Biometrica Eschews "Warm-and-Fuzzy" in Medical Research

2001-04-23T04:00:00Z

Developing a Drosophila high throughput screen technology, #949A.

2001-03-21T04:00:00Z

S. Dept. of Functional Genomics, Novartis Biomedical Research Institute (Summit NJ)

Fully Automated Instrumentation for Gene Function Analysis in D. melanogaster, #956B (p. a330).

2001-03-21T04:00:00Z

Union Biometrica, Inc. (Holliston, MA), *Cambria Biosciences, LLC (Bedford, MA)

Drug Discovery Tutorial: Automated Testing and Model Organisms

2001-02-15T05:00:00Z

No worm's pace here

2000-11-29T05:00:00Z

Boy Is My Bursa Red: Automated Detection and Sorting of Fluorescent Stained C. elegans Males From a Mixed Population, #89.

2000-06-09T04:00:00Z

Union Biometrica, Inc. (Holliston, MA).

Adapting a manual clonal screen to semi-automation, #858.

1999-06-02T04:00:00Z

1) MCB, Harvard University, Cambridge, MA, 02138., 2) Union Biometrica, 19 Ward St., Holliston, MA 01746, 3) Axys, Nemapharm Group, South San Francisco, CA

Automated sorting and dispensing of C. elegans to wells of microtiter plates, #443.

1999-06-02T04:00:00Z

1) Axys Pharmaceuticals, NemaPharm Group, South San Francisco, CA, 2) Union Biometrica, Inc., Holliston, MA.

Animal Models of Disease

2011-09-29T20:35:11Z

Commentary: Classical genetics goes high-tech

2011-09-29T20:35:11Z

Automated fluorescence-activated collecting duct isolation for genomics and proteomics # 913.1.

2011-09-29T20:35:11Z

Pediatrics, University of Utah, 30 North 1900 East, SOM 4R312, Salt Lake City, Utah, 84108