The BioSorter platform is designed for the in-flow measurement of objects ranging in size from 1 – 1500 microns.

BioSorter

BioSorter Accessory Modules

A variety of optional modules are available to add specific enhanced capabilities to your BioSorter system including choices of lasers, larger sample cups and an auto-sampler.

FOCAs (Fluidic and Optics Core Assemblies)

Depending on your application(s) you can choose from a range of FOCAs including 250, 500, 1000 and 2000 micron as well as metal-free. For more details please see the main BioSorter pages above.

Fig 1 – interchangeable Fluidic and Optics Core Assemblies – FOCAs.

Laser Choices

BioSorter can accommodate up to four excitation lasers. The 488nm is standard but many customers also add a 405nm, 445nm, 561nm, 640nm or 660nm laser. Please inquire if your research requires a different excitation wavelength. These lasers can either be added as part of the initial factory build or later as a field upgrade.

Fig 2 – Laser choices

Sample Cups

Samples are introduced via a standard 50 ml conical tube (40 ml working volume) with suspended stirrer. For larger samples optional 750 ml and 1500 ml stirred sample cups are available.

Fig 3 – Stirred sample cups

LP Sampler™ (Large Particle Sampler)

This is the only automated sample introduction system designed specifically for gentle handling of large, fragile objects such as.

  • delicate cells/clusters (stem cell clusters, neurospheres, tumor spheroids)
  • viable small multicellular model organisms (nematodes, Drosophila, zebrafish larvae)
  • beads used as micro-carriers for clonal libraries
  • seeds and fragile plant tissue

Fig 4 – LP Sampler

This autosampler is ideal for objects ranging from 10 to 1500 microns in diameter. It is capable of gently aspirating those samples from multiwell plates or a petri dish and delivering them intact to Union Biometrica’s COPAS FP™ BioSorter® or VAST BioImager™ systems. It has the flexibility to handle a variety of standard or deep well plates including 24, 96 and 384.

Rotating Sample Chamber and Non-metallic FOCA

The Rotating Sample Chamber is designed specifically for handling extremely delicate samples (adipocytes, plant protoplasts, etc.) and for samples that tend to either float or sink in a sample cup with normal stirring-type agitation. Chamber can be used with 250 & 500 micron FOCAs (order separately).

The 500 Micron Metallic-free FOCA is designed for handling adipocytes and other delicate samples where contact with metal surfaces must be avoided.  This FOCA can accommodate objects 20 – 400 microns in diameter and is optimized for objects 40 – 300 microns in diameter.  Use with the Rotating Sample Introduction Chamber with Integrated Agitator (order separately) for a completely metal-free sample path.  Typical use with a mouse adipocyte preparation enables analyzing 100 objects/second and dispensing intact adipocytes constituting 10% of the preparation.

Fig 5 – Rotating sample cup for fragile samples and samples that float or sink

 

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